Strain Fitness in Pseudomonas putida KT2440 around PP_2032

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,308,827	-		PP_2031	0.75	-0.8
2,308,877	-		PP_2031	0.80	+0.4
2,308,877	-		PP_2031	0.80	+0.0
2,309,348	+		PP_2032	0.30	+0.0
2,309,348	+		PP_2032	0.30	-2.4
2,309,372	+		PP_2032	0.32	-1.3
2,309,372	+		PP_2032	0.32	-1.5
2,309,372	+		PP_2032	0.32	-0.8
2,309,373	-		PP_2032	0.32	-0.7
2,309,373	-		PP_2032	0.32	+1.2
2,309,488	+		PP_2032	0.44	-0.7
2,309,516	-				+0.9
2,309,558	+		PP_2032	0.52	+0.3
2,309,559	-		PP_2032	0.52	+0.0
2,309,621	+		PP_2032	0.58	+1.0
2,309,622	-		PP_2032	0.58	-1.8
2,309,622	-		PP_2032	0.58	-1.1
2,309,622	-		PP_2032	0.58	-0.7
2,309,725	-		PP_2032	0.69	-1.3
2,309,733	+		PP_2032	0.70	-1.9
2,309,733	+		PP_2032	0.70	-0.3
2,309,734	-		PP_2032	0.70	-0.9
2,309,965	+				+0.5
2,309,965	+				-1.8
2,309,965	+				-1.9
2,309,977	-				-0.1
2,310,085	-				+0.8
2,310,085	-				-1.1
2,310,089	+				-0.0
2,310,089	+				-2.1
2,310,090	-				-0.7
2,310,090	-				-0.3
2,310,090	-				-1.8
2,310,090	-				-0.5
2,310,132	+				+0.6
2,310,286	-				-0.2
2,310,385	+		PP_2033	0.10	+0.4
2,310,386	-		PP_2033	0.10	+1.5
2,310,386	-		PP_2033	0.10	-1.5
2,310,428	-		PP_2033	0.14	+0.3
2,310,428	-		PP_2033	0.14	+0.5
2,310,428	-		PP_2033	0.14	-0.7
2,310,478	+		PP_2033	0.17	-0.5
2,310,478	+		PP_2033	0.17	+0.4
2,310,478	+		PP_2033	0.17	+1.4
2,310,479	-		PP_2033	0.17	-1.5
2,310,524	-		PP_2033	0.21	+0.1
2,310,524	-		PP_2033	0.21	+1.2
2,310,524	-		PP_2033	0.21	-2.4
2,310,806	+		PP_2033	0.42	+0.2
2,310,806	+		PP_2033	0.42	-0.1
2,310,807	-		PP_2033	0.42	+0.7
2,310,918	+		PP_2033	0.51	+0.1
2,310,919	-		PP_2033	0.51	+0.5
2,310,975	+		PP_2033	0.55	+0.5