Strain Fitness in Pseudomonas putida KT2440 around PP_2016

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,286,109	+		PP_2014	0.44	+0.2
2,286,110	-		PP_2014	0.44	+0.9
2,286,110	-		PP_2014	0.44	-0.3
2,286,115	+		PP_2014	0.45	-2.0
2,286,115	+		PP_2014	0.45	+0.9
2,286,116	-		PP_2014	0.45	+0.7
2,286,116	-		PP_2014	0.45	+0.9
2,286,116	-		PP_2014	0.45	-2.1
2,286,223	+		PP_2014	0.57	+1.0
2,286,223	+		PP_2014	0.57	+1.3
2,286,323	-		PP_2014	0.68	+1.7
2,286,367	+		PP_2014	0.73	+0.0
2,286,367	+		PP_2014	0.73	+2.4
2,286,368	-		PP_2014	0.73	-2.1
2,286,470	+		PP_2014	0.85	-0.7
2,286,471	-		PP_2014	0.85	+0.0
2,286,588	+				+0.1
2,286,616	+				+0.2
2,286,616	+				-0.5
2,286,616	+				+1.1
2,286,616	+				-0.1
2,286,617	-				-0.3
2,286,617	-				+0.5
2,286,617	-				-0.8
2,286,617	-				+1.8
2,286,617	-				+2.8
2,286,617	-				+1.5
2,286,801	-		PP_2015	0.70	+1.0
2,286,870	+				+1.3
2,286,870	+				-0.3
2,286,871	-				+1.1
2,286,915	-				+1.0
2,286,940	+				-0.9
2,287,010	-		PP_2016	0.16	+0.9
2,287,072	+		PP_2016	0.24	-2.5
2,287,072	+		PP_2016	0.24	+0.7
2,287,141	+		PP_2016	0.32	+1.1
2,287,208	-		PP_2016	0.40	+0.1
2,287,633	+				+0.8
2,287,633	+				+0.2
2,287,672	+				+0.3
2,287,672	+				-0.0
2,287,673	-				+0.1
2,287,673	-				+1.1
2,287,673	-				-0.5
2,287,681	+				-0.3
2,287,682	-				-0.9
2,287,745	+				+0.8
2,287,746	-				+0.1
2,287,757	+				-0.1
2,287,758	-				+0.4
2,287,772	+				+0.8
2,287,955	+				+0.4
2,287,955	+				-1.5
2,287,956	-				+0.3
2,287,956	-				-1.0
2,288,063	+		PP_2017	0.13	+0.4
2,288,063	+		PP_2017	0.13	+1.3
2,288,123	+		PP_2017	0.15	-1.1
2,288,123	+		PP_2017	0.15	-1.3
2,288,123	+		PP_2017	0.15	+0.4
2,288,124	-		PP_2017	0.15	-2.6
2,288,124	-		PP_2017	0.15	-2.3
2,288,124	-		PP_2017	0.15	-1.6
2,288,124	-		PP_2017	0.15	-0.0
2,288,124	-		PP_2017	0.15	+0.7
2,288,126	+		PP_2017	0.15	-2.0
2,288,127	-		PP_2017	0.15	-0.5
2,288,127	-		PP_2017	0.15	+0.5
2,288,288	+		PP_2017	0.21	-0.6
2,288,375	+		PP_2017	0.25	-1.2
2,288,376	-		PP_2017	0.25	-0.2
2,288,376	-		PP_2017	0.25	-0.7
2,288,456	+		PP_2017	0.28	-0.8
2,288,468	+		PP_2017	0.28	-1.4