Strain Fitness in Pseudomonas putida KT2440 around PP_1982

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,244,980	+		PP_1981	0.54	+0.9
2,244,980	+		PP_1981	0.54	-0.2
2,244,980	+		PP_1981	0.54	+0.1
2,245,134	+		PP_1981	0.70	-0.6
2,245,188	-		PP_1981	0.75	-0.5
2,245,451	-				+0.2
2,245,528	+				+1.0
2,245,529	-				-1.7
2,245,538	+				+2.1
2,245,603	-				+1.5
2,245,664	-				-0.8
2,245,695	+				-2.8
2,245,732	-				+1.5
2,245,739	+				-0.4
2,245,739	+				+0.1
2,245,740	-				-0.5
2,245,761	+				+0.2
2,245,762	-				-1.0
2,245,773	+				+0.8
2,245,773	+				+1.1
2,245,774	-				-0.1
2,245,873	+		PP_1982	0.25	+1.4
2,245,882	+		PP_1982	0.27	+0.6
2,245,882	+		PP_1982	0.27	-1.5
2,245,882	+		PP_1982	0.27	+1.8
2,245,883	-		PP_1982	0.27	+0.2
2,245,883	-		PP_1982	0.27	+0.3
2,245,883	-		PP_1982	0.27	-0.1
2,245,884	+		PP_1982	0.27	+1.0
2,245,885	-		PP_1982	0.27	+0.4
2,245,885	-		PP_1982	0.27	+2.1
2,246,029	+		PP_1982	0.59	-0.3
2,246,281	-				+1.3
2,246,306	+				+0.8
2,246,306	+				+0.8
2,246,354	-				-1.8
2,246,381	+				-0.4
2,246,577	-				-2.2
2,246,592	-				-0.2
2,246,677	+				-0.1
2,246,677	+		PP_1983	0.11	-0.8
2,246,677	+		PP_1983	0.11	-0.2
2,246,678	-		PP_1983	0.11	-0.4
2,246,791	+		PP_1983	0.15	-1.7
2,246,791	+		PP_1983	0.15	-1.9
2,246,792	-		PP_1983	0.15	+0.1
2,246,792	-		PP_1983	0.15	+0.4
2,246,977	+		PP_1983	0.20	-0.6
2,246,977	+		PP_1983	0.20	-1.9
2,246,978	-		PP_1983	0.21	-0.8
2,246,978	-		PP_1983	0.21	-2.0
2,246,978	-		PP_1983	0.21	+0.6
2,246,978	-		PP_1983	0.21	-1.9
2,247,159	-		PP_1983	0.26	+2.3