Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1962 and PP_1963 are separated by 82 nucleotides PP_1963 and PP_1964 overlap by 35 nucleotides
PP_1962: PP_1962 - Site-specific recombinase, phage integrase family, at 2,222,618 to 2,224,546
_1962
PP_1963: PP_1963 - conserved protein of unknown function, at 2,224,629 to 2,226,632
_1963
PP_1964: PP_1964 - putative dGMP, dTMP, dhydroxyCMP kinase, at 2,226,598 to 2,227,401
_1964
Position (kb)
2224
2225
2226
2227 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2223.852 kb on + strand, within PP_1962 at 2223.879 kb on + strand, within PP_1962 at 2224.031 kb on - strand, within PP_1962 at 2224.046 kb on - strand, within PP_1962 at 2224.339 kb on - strand, within PP_1962 at 2224.454 kb on + strand at 2224.876 kb on + strand, within PP_1963 at 2224.911 kb on + strand, within PP_1963 at 2224.911 kb on + strand, within PP_1963 at 2224.912 kb on - strand, within PP_1963 at 2225.090 kb on + strand, within PP_1963 at 2225.135 kb on + strand, within PP_1963 at 2225.243 kb on + strand, within PP_1963 at 2225.258 kb on + strand, within PP_1963 at 2225.776 kb on + strand, within PP_1963 at 2225.828 kb on - strand, within PP_1963 at 2226.018 kb on + strand, within PP_1963 at 2226.019 kb on - strand, within PP_1963 at 2226.263 kb on + strand, within PP_1963 at 2226.293 kb on - strand, within PP_1963 at 2226.349 kb on - strand, within PP_1963 at 2226.373 kb on + strand, within PP_1963 at 2226.560 kb on - strand at 2227.443 kb on - strand at 2227.512 kb on + strand at 2227.526 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,223,852 + PP_1962 0.64 +0.4 2,223,879 + PP_1962 0.65 -0.4 2,224,031 - PP_1962 0.73 -1.0 2,224,046 - PP_1962 0.74 +1.4 2,224,339 - PP_1962 0.89 +2.0 2,224,454 + +0.4 2,224,876 + PP_1963 0.12 +1.1 2,224,911 + PP_1963 0.14 +0.1 2,224,911 + PP_1963 0.14 +1.7 2,224,912 - PP_1963 0.14 +0.3 2,225,090 + PP_1963 0.23 -2.2 2,225,135 + PP_1963 0.25 -0.9 2,225,243 + PP_1963 0.31 -0.5 2,225,258 + PP_1963 0.31 +0.5 2,225,776 + PP_1963 0.57 +0.5 2,225,828 - PP_1963 0.60 -0.5 2,226,018 + PP_1963 0.69 +0.3 2,226,019 - PP_1963 0.69 -2.2 2,226,263 + PP_1963 0.82 -1.4 2,226,293 - PP_1963 0.83 -1.1 2,226,349 - PP_1963 0.86 -1.7 2,226,373 + PP_1963 0.87 -1.1 2,226,560 - +1.5 2,227,443 - -3.1 2,227,512 + +3.3 2,227,526 + -0.9
Or see this region's nucleotide sequence