Strain Fitness in Pseudomonas putida KT2440 around PP_1956

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,212,298	+		PP_1955	0.35	+0.8
2,212,299	-		PP_1955	0.35	+0.4
2,212,338	-		PP_1955	0.38	-2.2
2,212,376	-		PP_1955	0.41	-0.2
2,212,412	+		PP_1955	0.44	-0.8
2,212,413	-		PP_1955	0.44	-1.4
2,212,413	-		PP_1955	0.44	+1.1
2,212,457	+		PP_1955	0.48	-0.1
2,212,457	+		PP_1955	0.48	+0.7
2,212,457	+		PP_1955	0.48	-0.5
2,212,458	-		PP_1955	0.48	-0.4
2,212,458	-		PP_1955	0.48	+0.2
2,212,458	-		PP_1955	0.48	+1.2
2,212,475	+		PP_1955	0.49	-0.4
2,212,482	-		PP_1955	0.50	-0.7
2,212,836	+		PP_1955	0.78	+0.4
2,212,836	+				+1.0
2,212,837	-		PP_1955	0.78	+0.3
2,212,837	-		PP_1955	0.78	-0.6
2,212,855	+		PP_1955	0.80	-1.9
2,212,930	+				+1.1
2,212,945	+		PP_1955	0.87	+0.9
2,212,945	+		PP_1955	0.87	+0.9
2,212,946	-		PP_1955	0.87	-1.5
2,213,076	+				+0.2
2,213,127	+				+0.2
2,213,128	-				-1.1
2,213,130	+				+0.8
2,213,183	+				+1.1
2,213,184	-				-0.8
2,213,234	+				+0.8
2,213,383	+		PP_1956	0.21	-1.1
2,213,383	+		PP_1956	0.21	-0.7
2,213,384	-		PP_1956	0.22	+0.8
2,213,384	-		PP_1956	0.22	-0.6
2,213,434	-		PP_1956	0.27	-0.2
2,213,446	+		PP_1956	0.28	-2.2
2,213,484	+		PP_1956	0.32	+0.7
2,213,484	+		PP_1956	0.32	-0.2
2,213,484	+		PP_1956	0.32	-1.0
2,213,632	+		PP_1956	0.47	+0.5
2,213,633	-		PP_1956	0.47	-0.2
2,213,746	-		PP_1956	0.59	+0.2
2,213,821	-		PP_1956	0.67	+0.6
2,213,821	-		PP_1956	0.67	-1.0
2,213,909	-		PP_1956	0.76	-1.6
2,213,973	-		PP_1956	0.82	-0.3
2,214,021	+		PP_1956	0.87	+0.8
2,214,031	+		PP_1956	0.88	-0.8
2,214,137	+				-2.1
2,214,141	+				+1.6
2,214,192	-				+0.1
2,214,199	-				-0.0
2,214,217	+				+0.4
2,214,217	+				+0.5
2,214,252	+				-1.2
2,214,286	+				-1.7
2,214,286	+				-0.7
2,214,286	+				-0.9
2,214,290	-				+1.2
2,214,353	-		PP_1957	0.13	-1.0
2,214,354	+		PP_1957	0.13	-0.2
2,214,354	+		PP_1957	0.13	-0.7
2,214,354	+		PP_1957	0.13	-1.8
2,214,354	+		PP_1957	0.13	-2.0
2,214,354	+		PP_1957	0.13	-1.0
2,214,354	+		PP_1957	0.13	-1.4
2,214,354	+		PP_1957	0.13	-0.8
2,214,354	+		PP_1957	0.13	+0.0
2,214,355	-		PP_1957	0.13	+0.0
2,214,355	-		PP_1957	0.13	-0.4
2,214,355	-		PP_1957	0.13	+0.5
2,214,355	-		PP_1957	0.13	+0.3
2,214,355	-		PP_1957	0.13	-0.7
2,214,355	-		PP_1957	0.13	+0.1
2,214,361	-		PP_1957	0.13	-0.8
2,214,361	-		PP_1957	0.13	-1.0
2,214,400	+		PP_1957	0.18	+0.5
2,214,544	-		PP_1957	0.33	+0.2
2,214,650	+		PP_1957	0.44	-1.6
2,214,651	-		PP_1957	0.44	+0.7
2,214,651	-		PP_1957	0.44	+0.7
2,214,716	-		PP_1957	0.51	+0.1
2,214,751	+		PP_1957	0.54	-1.1
2,214,752	-		PP_1957	0.54	-0.4
2,214,759	+		PP_1957	0.55	+1.1
2,214,759	+		PP_1957	0.55	-0.4
2,214,759	+		PP_1957	0.55	+1.1
2,214,765	+		PP_1957	0.56	-0.5
2,214,766	-		PP_1957	0.56	+0.2
2,214,766	-		PP_1957	0.56	+0.8
2,214,766	-		PP_1957	0.56	+0.1
2,214,799	+		PP_1957	0.59	-0.5
2,214,800	+		PP_1957	0.59	-1.3
2,214,801	-		PP_1957	0.60	+0.2
2,214,801	-		PP_1957	0.60	-1.0
2,214,801	-		PP_1957	0.60	-0.3
2,214,801	-		PP_1957	0.60	+0.2
2,214,801	-		PP_1957	0.60	+0.4
2,214,801	-		PP_1957	0.60	-1.2
2,214,801	-		PP_1957	0.60	-0.9
2,214,837	+		PP_1957	0.63	-0.3
2,214,901	+				+1.6
2,214,902	-		PP_1957	0.70	-0.1
2,214,957	+		PP_1957	0.76	-1.2
2,214,986	-		PP_1957	0.79	+0.8
2,215,011	+		PP_1957	0.82	+0.0
2,215,012	-		PP_1957	0.82	-1.8
2,215,012	-		PP_1957	0.82	+0.0
2,215,054	+		PP_1957	0.86	-1.0
2,215,054	+		PP_1957	0.86	+0.7
2,215,055	-		PP_1957	0.86	-2.2
2,215,126	+				+0.4
2,215,127	-				-0.9