Strain Fitness in Pseudomonas putida KT2440 around PP_1950

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,205,594	+		PP_1949	0.47	-0.8
2,205,595	-		PP_1949	0.47	-0.9
2,205,648	-		PP_1949	0.50	+0.9
2,205,648	-		PP_1949	0.50	-0.6
2,205,663	-		PP_1949	0.51	-1.6
2,205,715	+		PP_1949	0.54	-0.3
2,205,716	-		PP_1949	0.54	-0.9
2,205,716	-		PP_1949	0.54	-1.1
2,205,802	+		PP_1949	0.59	+1.0
2,205,802	+		PP_1949	0.59	+1.8
2,205,803	-		PP_1949	0.59	-0.9
2,205,803	-		PP_1949	0.59	-0.1
2,205,803	-		PP_1949	0.59	+0.9
2,205,990	-		PP_1949	0.71	+0.1
2,206,021	-		PP_1949	0.72	+0.2
2,206,143	-		PP_1949	0.80	+0.7
2,206,143	-		PP_1949	0.80	+3.0
2,206,205	-		PP_1949	0.84	-1.7
2,206,266	-		PP_1949	0.87	+0.5
2,206,266	-		PP_1949	0.87	-0.5
2,206,347	+				+0.4
2,206,347	+				+1.2
2,206,347	+				+0.2
2,206,369	-				-1.5
2,206,384	-				+1.4
2,206,481	+				+1.2
2,206,522	+				+0.3
2,206,666	-				+0.7
2,206,759	-		PP_1950	0.16	-0.5
2,206,804	-		PP_1950	0.20	+2.5
2,206,982	-		PP_1950	0.35	-1.0
2,207,058	-		PP_1950	0.41	-0.6
2,207,093	-		PP_1950	0.44	-0.8
2,207,123	+		PP_1950	0.47	+0.5
2,207,124	-		PP_1950	0.47	-3.0
2,207,167	-		PP_1950	0.51	-0.5
2,207,167	-		PP_1950	0.51	+1.0
2,207,192	-		PP_1950	0.53	+1.5
2,207,234	-		PP_1950	0.56	+0.3
2,207,234	-		PP_1950	0.56	+0.4
2,207,423	+		PP_1950	0.72	-1.5
2,207,423	+		PP_1950	0.72	+0.8
2,207,474	+		PP_1950	0.77	-0.0
2,207,509	-		PP_1950	0.80	+0.3
2,207,509	-		PP_1950	0.80	-1.4
2,207,509	-				+0.8
2,207,565	+		PP_1950	0.84	-1.3
2,207,565	+		PP_1950	0.84	-2.2
2,207,565	+		PP_1950	0.84	-0.7
2,207,566	-		PP_1950	0.84	-0.2
2,207,566	-		PP_1950	0.84	-1.4
2,207,675	+				+1.9
2,207,675	+				+1.2
2,207,733	-				-0.4
2,207,733	-				-0.5
2,207,835	+				+1.4
2,207,857	-				-0.2
2,207,862	+				-1.8
2,207,862	+				-0.4
2,207,862	+				-2.0
2,207,863	-				-1.1
2,208,013	-		PP_1951	0.27	+1.4
2,208,019	+		PP_1951	0.28	+2.2
2,208,025	+		PP_1951	0.29	-1.0
2,208,026	-		PP_1951	0.29	+0.3
2,208,026	-		PP_1951	0.29	+0.1
2,208,139	+		PP_1951	0.42	-1.0
2,208,140	-		PP_1951	0.42	-2.4
2,208,140	-		PP_1951	0.42	-0.8
2,208,153	-		PP_1951	0.44	-0.7
2,208,192	+		PP_1951	0.49	+0.0
2,208,192	+		PP_1951	0.49	-1.1
2,208,192	+		PP_1951	0.49	-0.2
2,208,193	-		PP_1951	0.49	-0.6
2,208,241	+		PP_1951	0.55	+0.2
2,208,259	+		PP_1951	0.57	-1.3
2,208,259	+		PP_1951	0.57	+1.3
2,208,345	+		PP_1951	0.67	-0.5
2,208,346	-		PP_1951	0.67	+0.3
2,208,346	-		PP_1951	0.67	-2.4
2,208,346	-		PP_1951	0.67	-0.3
2,208,530	+		PP_1951	0.89	+1.1
2,208,531	-		PP_1951	0.90	-0.2
2,208,615	-				-2.1
2,208,628	-				-1.4
2,208,633	-				+0.6