Strain Fitness in Pseudomonas putida KT2440 around PP_1948

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
2,202,370	+				-0.2
2,202,396	+				+1.5
2,202,397	-				+0.5
2,202,398	+				-0.3
2,202,405	-				+1.5
2,202,584	+		PP_1947	0.26	-1.2
2,202,585	-		PP_1947	0.26	+0.3
2,202,585	-		PP_1947	0.26	+0.1
2,202,608	+		PP_1947	0.29	-0.1
2,202,609	-		PP_1947	0.29	+0.2
2,202,770	+		PP_1947	0.45	-1.6
2,202,770	+		PP_1947	0.45	+0.7
2,202,771	-		PP_1947	0.45	-0.5
2,202,804	-		PP_1947	0.48	+1.8
2,202,804	-		PP_1947	0.48	-1.2
2,202,804	-		PP_1947	0.48	+0.3
2,202,980	+		PP_1947	0.66	+2.2
2,202,984	-		PP_1947	0.67	-0.2
2,203,041	-		PP_1947	0.72	+0.3
2,203,218	-				-0.4
2,203,218	-				-1.5
2,203,396	+				-1.1
2,203,396	+				+0.2
2,203,411	+				-0.7
2,203,415	+				+0.1
2,203,435	+				+0.5
2,203,435	+				+0.8
2,203,436	-				-1.1
2,203,436	-				-0.8
2,203,437	+				-1.4
2,203,509	+		PP_1948	0.13	-1.8
2,203,510	-		PP_1948	0.13	+0.6
2,203,579	-		PP_1948	0.17	+0.5
2,203,579	-		PP_1948	0.17	-1.6
2,203,579	-		PP_1948	0.17	-1.0
2,203,592	+		PP_1948	0.18	+2.0
2,203,592	+		PP_1948	0.18	+1.5
2,203,593	-		PP_1948	0.18	-1.9
2,203,709	-		PP_1948	0.26	-0.8
2,203,711	+		PP_1948	0.26	-0.2
2,203,765	+		PP_1948	0.30	+1.1
2,203,765	+		PP_1948	0.30	-1.6
2,203,766	-		PP_1948	0.30	+0.0
2,203,774	+		PP_1948	0.30	-2.0
2,203,774	+		PP_1948	0.30	-0.8
2,203,774	+		PP_1948	0.30	+0.8
2,203,774	+		PP_1948	0.30	-2.0
2,203,774	+		PP_1948	0.30	+1.1
2,203,774	+		PP_1948	0.30	-0.9
2,203,775	-		PP_1948	0.30	+0.1
2,203,775	-		PP_1948	0.30	+0.2
2,203,775	-		PP_1948	0.30	-1.0
2,203,776	+		PP_1948	0.31	-1.8
2,203,776	+		PP_1948	0.31	-0.4
2,203,776	+		PP_1948	0.31	+1.1
2,203,777	-		PP_1948	0.31	+0.9
2,203,777	-		PP_1948	0.31	+0.7
2,203,970	+		PP_1948	0.44	+0.1
2,204,294	+		PP_1948	0.66	+1.0
2,204,295	-		PP_1948	0.66	-0.7
2,204,378	-		PP_1948	0.71	-0.8
2,204,452	+		PP_1948	0.76	-0.5
2,204,452	+		PP_1948	0.76	-0.2
2,204,452	+		PP_1948	0.76	-0.3
2,204,452	+		PP_1948	0.76	+1.1
2,204,452	+		PP_1948	0.76	+0.2
2,204,452	+		PP_1948	0.76	+1.4
2,204,453	-		PP_1948	0.76	-0.3
2,204,453	-		PP_1948	0.76	-0.9
2,204,453	-		PP_1948	0.76	-2.3
2,204,453	-		PP_1948	0.76	+0.5
2,204,456	+		PP_1948	0.77	+0.8
2,204,456	+		PP_1948	0.77	+1.5
2,204,457	-		PP_1948	0.77	+0.5
2,204,457	-		PP_1948	0.77	+1.2
2,204,470	+		PP_1948	0.77	-0.3
2,204,477	-		PP_1948	0.78	-0.8
2,204,606	+		PP_1948	0.87	-2.0
2,204,607	-		PP_1948	0.87	+1.8
2,204,607	-		PP_1948	0.87	+1.7
2,204,669	-				+2.5
2,204,669	-				-1.7
2,204,669	-				+0.8
2,204,680	+				-0.9
2,204,680	+				-0.1
2,204,681	-				+1.2
2,204,767	+				+0.3
2,204,767	+				+0.2
2,204,768	-				+0.4
2,204,768	-				+1.3
2,204,768	-				+1.3
2,204,768	-				-1.3
2,204,768	-				+0.3
2,204,845	+				+0.4
2,204,846	-				-0.1
2,204,851	+				-0.0
2,204,851	+				-2.7
2,204,851	+				-2.0
2,204,851	+				+0.3
2,204,851	+				-0.7
2,204,852	-				-1.4
2,204,852	-				-0.0
2,204,852	-				+0.9
2,204,852	-				+1.4
2,204,855	+				-1.7
2,204,855	+				+0.4
2,204,855	+				+0.6
2,204,855	+				+0.3
2,204,855	+				+0.2
2,204,855	+				-0.6
2,204,855	+				-2.3
2,204,855	+				-1.2
2,204,855	+				+1.1
2,204,856	-				+0.3
2,204,856	-				+0.3
2,204,856	-				+1.3
2,204,856	-				+0.3
2,204,856	-				-1.1
2,204,856	-				-0.3
2,204,856	-				+0.4
2,204,927	+				+1.2
2,204,927	+				+1.4
2,204,928	-				+1.1
2,204,928	-				-0.5
2,205,022	+		PP_1949	0.12	-1.3
2,205,042	+		PP_1949	0.13	-1.5
2,205,043	-		PP_1949	0.13	+0.6
2,205,060	+		PP_1949	0.14	+1.7
2,205,060	+		PP_1949	0.14	+0.9
2,205,060	+		PP_1949	0.14	-0.6
2,205,060	+		PP_1949	0.14	+0.1
2,205,060	+		PP_1949	0.14	+0.9
2,205,060	+		PP_1949	0.14	+0.1
2,205,060	+				+2.5
2,205,060	+		PP_1949	0.14	+0.5
2,205,060	+		PP_1949	0.14	+1.0
2,205,061	-		PP_1949	0.14	-0.6
2,205,061	-		PP_1949	0.14	-0.2
2,205,061	-		PP_1949	0.14	-1.3
2,205,061	-				+0.6
2,205,061	-		PP_1949	0.14	+1.3
2,205,064	+		PP_1949	0.15	+0.7
2,205,064	+		PP_1949	0.15	+0.1
2,205,064	+		PP_1949	0.15	-1.7
2,205,064	+		PP_1949	0.15	+2.0
2,205,064	+		PP_1949	0.15	-1.7
2,205,064	+		PP_1949	0.15	-1.4
2,205,064	+		PP_1949	0.15	-0.5
2,205,065	-		PP_1949	0.15	-0.8
2,205,065	-		PP_1949	0.15	-0.3
2,205,065	-		PP_1949	0.15	+1.8
2,205,065	-		PP_1949	0.15	-0.4
2,205,065	-		PP_1949	0.15	-0.8
2,205,065	-		PP_1949	0.15	+1.1
2,205,065	-		PP_1949	0.15	+1.0
2,205,065	-		PP_1949	0.15	-0.9
2,205,065	-		PP_1949	0.15	-0.4
2,205,065	-		PP_1949	0.15	-1.5
2,205,065	-		PP_1949	0.15	-1.1
2,205,065	-		PP_1949	0.15	+3.0
2,205,065	-		PP_1949	0.15	-1.0
2,205,118	+		PP_1949	0.18	-0.9
2,205,118	+		PP_1949	0.18	-1.0
2,205,118	+		PP_1949	0.18	-1.1
2,205,119	-		PP_1949	0.18	-0.6
2,205,133	-		PP_1949	0.19	+0.9
2,205,133	-		PP_1949	0.19	+0.1
2,205,133	-		PP_1949	0.19	-0.9
2,205,133	-		PP_1949	0.19	+0.9
2,205,142	+		PP_1949	0.19	+0.3
2,205,143	-		PP_1949	0.19	-0.2
2,205,162	-		PP_1949	0.20	-0.8
2,205,162	-		PP_1949	0.20	+0.2
2,205,203	-		PP_1949	0.23	+0.6
2,205,399	+		PP_1949	0.35	+1.1
2,205,399	+		PP_1949	0.35	+0.9
2,205,399	+		PP_1949	0.35	+0.6
2,205,400	-		PP_1949	0.35	-0.5
2,205,400	-		PP_1949	0.35	-0.8
2,205,446	+		PP_1949	0.38	-1.2
2,205,533	-		PP_1949	0.43	-1.1
2,205,594	+		PP_1949	0.47	-0.8
2,205,595	-		PP_1949	0.47	-0.9
2,205,648	-		PP_1949	0.50	+0.9
2,205,648	-		PP_1949	0.50	-0.6
2,205,663	-		PP_1949	0.51	-1.6
2,205,715	+		PP_1949	0.54	-0.3
2,205,716	-		PP_1949	0.54	-0.9
2,205,716	-		PP_1949	0.54	-1.1
2,205,802	+		PP_1949	0.59	+1.0
2,205,802	+		PP_1949	0.59	+1.8