Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5482 and PP_1920 are separated by 163 nucleotides PP_1920 and PP_5483 are separated by 67 nucleotides
PP_5482: PP_5482 - protein of unknown function, at 2,163,140 to 2,164,153
_5482
PP_1920: PP_1920 - conserved protein of unknown function, at 2,164,317 to 2,165,537
_1920
PP_5483: PP_5483 - conserved protein of unknown function, at 2,165,605 to 2,166,156
_5483
Position (kb)
2164
2165
2166 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2163.349 kb on + strand, within PP_5482 at 2163.383 kb on - strand, within PP_5482 at 2163.422 kb on + strand, within PP_5482 at 2163.422 kb on + strand, within PP_5482 at 2163.452 kb on + strand, within PP_5482 at 2163.610 kb on + strand, within PP_5482 at 2163.644 kb on - strand, within PP_5482 at 2163.646 kb on - strand, within PP_5482 at 2163.786 kb on + strand, within PP_5482 at 2163.787 kb on - strand, within PP_5482 at 2163.897 kb on + strand, within PP_5482 at 2163.980 kb on + strand, within PP_5482 at 2163.984 kb on - strand, within PP_5482 at 2163.984 kb on - strand, within PP_5482 at 2164.147 kb on + strand at 2164.148 kb on - strand at 2164.223 kb on + strand at 2164.364 kb on + strand at 2164.424 kb on + strand at 2164.482 kb on + strand, within PP_1920 at 2164.599 kb on - strand, within PP_1920 at 2164.632 kb on + strand, within PP_1920 at 2164.723 kb on - strand, within PP_1920 at 2164.738 kb on + strand, within PP_1920 at 2164.738 kb on + strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.739 kb on - strand, within PP_1920 at 2164.852 kb on + strand, within PP_1920 at 2164.931 kb on + strand, within PP_1920 at 2165.143 kb on - strand, within PP_1920 at 2165.232 kb on + strand, within PP_1920 at 2165.232 kb on - strand, within PP_1920 at 2165.266 kb on + strand, within PP_1920 at 2165.344 kb on - strand, within PP_1920 at 2165.348 kb on + strand, within PP_1920 at 2165.351 kb on - strand, within PP_1920 at 2165.351 kb on - strand, within PP_1920 at 2165.595 kb on - strand at 2165.785 kb on - strand, within PP_5483 at 2165.919 kb on + strand, within PP_5483 at 2166.133 kb on + strand at 2166.133 kb on + strand at 2166.158 kb on + strand at 2166.158 kb on + strand at 2166.159 kb on - strand at 2166.159 kb on - strand at 2166.182 kb on + strand at 2166.190 kb on + strand at 2166.191 kb on - strand at 2166.250 kb on + strand at 2166.250 kb on + strand at 2166.278 kb on + strand at 2166.278 kb on + strand at 2166.287 kb on + strand at 2166.288 kb on - strand at 2166.295 kb on + strand at 2166.296 kb on - strand at 2166.314 kb on - strand at 2166.319 kb on - strand at 2166.319 kb on - strand at 2166.380 kb on + strand at 2166.406 kb on + strand at 2166.406 kb on + strand at 2166.457 kb on - strand at 2166.490 kb on - strand at 2166.527 kb on + strand at 2166.528 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,163,349 + PP_5482 0.21 -0.2 2,163,383 - PP_5482 0.24 +1.5 2,163,422 + PP_5482 0.28 +1.9 2,163,422 + PP_5482 0.28 +1.2 2,163,452 + PP_5482 0.31 -0.2 2,163,610 + PP_5482 0.46 -0.7 2,163,644 - PP_5482 0.50 +0.9 2,163,646 - PP_5482 0.50 -0.7 2,163,786 + PP_5482 0.64 +1.3 2,163,787 - PP_5482 0.64 -0.7 2,163,897 + PP_5482 0.75 -0.5 2,163,980 + PP_5482 0.83 +0.9 2,163,984 - PP_5482 0.83 -1.1 2,163,984 - PP_5482 0.83 +2.0 2,164,147 + -1.8 2,164,148 - -0.7 2,164,223 + -0.5 2,164,364 + +0.9 2,164,424 + -0.9 2,164,482 + PP_1920 0.14 +0.5 2,164,599 - PP_1920 0.23 -0.7 2,164,632 + PP_1920 0.26 -2.0 2,164,723 - PP_1920 0.33 -0.2 2,164,738 + PP_1920 0.34 +0.2 2,164,738 + PP_1920 0.34 -2.0 2,164,739 - PP_1920 0.35 +0.5 2,164,739 - PP_1920 0.35 -1.9 2,164,739 - PP_1920 0.35 +0.4 2,164,852 + PP_1920 0.44 +0.8 2,164,931 + PP_1920 0.50 -0.8 2,165,143 - PP_1920 0.68 -0.1 2,165,232 + PP_1920 0.75 -0.5 2,165,232 - PP_1920 0.75 -1.8 2,165,266 + PP_1920 0.78 -2.0 2,165,344 - PP_1920 0.84 -0.7 2,165,348 + PP_1920 0.84 +0.3 2,165,351 - PP_1920 0.85 +1.2 2,165,351 - PP_1920 0.85 -1.8 2,165,595 - +0.1 2,165,785 - PP_5483 0.33 -0.9 2,165,919 + PP_5483 0.57 -1.6 2,166,133 + +1.4 2,166,133 + -1.0 2,166,158 + -1.3 2,166,158 + -1.3 2,166,159 - +0.8 2,166,159 - -0.5 2,166,182 + -0.7 2,166,190 + -0.3 2,166,191 - -2.2 2,166,250 + -0.5 2,166,250 + -0.0 2,166,278 + +0.1 2,166,278 + -1.7 2,166,287 + -0.0 2,166,288 - -0.1 2,166,295 + -1.6 2,166,296 - -2.0 2,166,314 - -0.0 2,166,319 - -1.4 2,166,319 - -0.5 2,166,380 + +0.5 2,166,406 + -0.8 2,166,406 + +0.2 2,166,457 - -1.0 2,166,490 - +1.1 2,166,527 + +1.1 2,166,528 - -0.9
Or see this region's nucleotide sequence