Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1811 and PP_1812 are separated by 121 nucleotides PP_1812 and PP_1813 are separated by 97 nucleotides PP_1813 and PP_1814 are separated by 36 nucleotides PP_1814 and PP_1815 are separated by 183 nucleotides
PP_1811: PP_1811 - UDP-N-acetylglucosamine-2-epimerase, at 2,038,337 to 2,039,479
_1811
PP_1812: PP_1812 - conserved protein of unknown function, at 2,039,601 to 2,039,801
_1812
PP_1813: PP_1813 - ComEA-related protein, at 2,039,899 to 2,040,237
_1813
PP_1814: PP_1814 - conserved protein of unknown function, at 2,040,274 to 2,040,441
_1814
PP_1815: PP_1815 - Orotidine 5'-phosphate decarboxylase, at 2,040,625 to 2,041,326
_1815
Position (kb)
2039
2040 Strain fitness (log2 ratio)
-2
-1
0
1 at 2038.627 kb on - strand, within PP_1811 at 2038.644 kb on + strand, within PP_1811 at 2038.644 kb on + strand, within PP_1811 at 2038.660 kb on - strand, within PP_1811 at 2038.660 kb on - strand, within PP_1811 at 2038.749 kb on + strand, within PP_1811 at 2038.749 kb on + strand, within PP_1811 at 2039.097 kb on + strand, within PP_1811 at 2039.097 kb on + strand at 2039.098 kb on - strand, within PP_1811 at 2039.098 kb on - strand, within PP_1811 at 2039.098 kb on - strand, within PP_1811 at 2039.126 kb on + strand, within PP_1811 at 2039.126 kb on + strand, within PP_1811 at 2039.127 kb on - strand, within PP_1811 at 2039.151 kb on + strand, within PP_1811 at 2039.151 kb on + strand, within PP_1811 at 2039.154 kb on + strand, within PP_1811 at 2039.154 kb on + strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.155 kb on - strand, within PP_1811 at 2039.307 kb on + strand, within PP_1811 at 2039.336 kb on + strand, within PP_1811 at 2039.581 kb on - strand at 2039.632 kb on + strand, within PP_1812 at 2039.688 kb on + strand, within PP_1812 at 2039.689 kb on - strand, within PP_1812 at 2039.811 kb on + strand at 2039.820 kb on - strand at 2039.917 kb on - strand at 2040.072 kb on + strand, within PP_1813 at 2040.120 kb on + strand, within PP_1813 at 2040.121 kb on - strand, within PP_1813 at 2040.189 kb on - strand, within PP_1813 at 2040.235 kb on + strand at 2040.235 kb on + strand at 2040.236 kb on - strand at 2040.313 kb on - strand, within PP_1814 at 2040.313 kb on - strand, within PP_1814 at 2040.322 kb on + strand, within PP_1814 at 2040.322 kb on + strand, within PP_1814 at 2040.322 kb on + strand, within PP_1814 at 2040.323 kb on - strand, within PP_1814 at 2040.409 kb on + strand, within PP_1814 at 2040.409 kb on + strand, within PP_1814 at 2040.431 kb on + strand at 2040.454 kb on + strand at 2040.477 kb on + strand at 2040.517 kb on + strand at 2040.517 kb on + strand at 2040.517 kb on + strand at 2040.518 kb on - strand at 2040.518 kb on - strand at 2040.518 kb on - strand at 2040.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 2,038,627 - PP_1811 0.25 +1.2 2,038,644 + PP_1811 0.27 -0.5 2,038,644 + PP_1811 0.27 -0.4 2,038,660 - PP_1811 0.28 -0.5 2,038,660 - PP_1811 0.28 +1.5 2,038,749 + PP_1811 0.36 -0.6 2,038,749 + PP_1811 0.36 -0.3 2,039,097 + PP_1811 0.66 +0.8 2,039,097 + +0.5 2,039,098 - PP_1811 0.67 +0.3 2,039,098 - PP_1811 0.67 +1.4 2,039,098 - PP_1811 0.67 -0.1 2,039,126 + PP_1811 0.69 -1.0 2,039,126 + PP_1811 0.69 -1.4 2,039,127 - PP_1811 0.69 +1.0 2,039,151 + PP_1811 0.71 -0.2 2,039,151 + PP_1811 0.71 +1.5 2,039,154 + PP_1811 0.71 -2.0 2,039,154 + PP_1811 0.71 +0.4 2,039,155 - PP_1811 0.72 +0.4 2,039,155 - PP_1811 0.72 -0.1 2,039,155 - PP_1811 0.72 -0.9 2,039,155 - PP_1811 0.72 -1.8 2,039,307 + PP_1811 0.85 +0.9 2,039,336 + PP_1811 0.87 +0.6 2,039,581 - -0.6 2,039,632 + PP_1812 0.15 -0.6 2,039,688 + PP_1812 0.43 -0.9 2,039,689 - PP_1812 0.44 -1.8 2,039,811 + +0.2 2,039,820 - -1.6 2,039,917 - -1.2 2,040,072 + PP_1813 0.51 -0.6 2,040,120 + PP_1813 0.65 +1.4 2,040,121 - PP_1813 0.65 -1.7 2,040,189 - PP_1813 0.86 -1.7 2,040,235 + +1.1 2,040,235 + -1.5 2,040,236 - -0.6 2,040,313 - PP_1814 0.23 +1.4 2,040,313 - PP_1814 0.23 +0.2 2,040,322 + PP_1814 0.29 -0.5 2,040,322 + PP_1814 0.29 +0.4 2,040,322 + PP_1814 0.29 -2.1 2,040,323 - PP_1814 0.29 -1.1 2,040,409 + PP_1814 0.80 -0.3 2,040,409 + PP_1814 0.80 +1.3 2,040,431 + -0.3 2,040,454 + -0.0 2,040,477 + -0.1 2,040,517 + +0.3 2,040,517 + -2.1 2,040,517 + -1.4 2,040,518 - -0.9 2,040,518 - -1.0 2,040,518 - +0.1 2,040,520 - -0.6
Or see this region's nucleotide sequence