Strain Fitness in Pseudomonas putida KT2440 around PP_1754

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
1,955,624	+		PP_1753	0.27	+0.1
1,955,624	+		PP_1753	0.27	-0.2
1,955,624	+		PP_1753	0.27	+2.1
1,955,625	-		PP_1753	0.27	-1.4
1,955,625	-		PP_1753	0.27	-0.2
1,955,625	-		PP_1753	0.27	+0.6
1,955,633	+		PP_1753	0.28	-2.8
1,955,682	+		PP_1753	0.33	-1.5
1,955,682	+		PP_1753	0.33	-1.6
1,955,837	+		PP_1753	0.49	-1.4
1,955,837	+		PP_1753	0.49	-1.4
1,955,837	+		PP_1753	0.49	-0.1
1,955,837	+		PP_1753	0.49	-0.5
1,955,837	+				+0.6
1,955,838	-		PP_1753	0.49	-1.5
1,955,838	-		PP_1753	0.49	-0.3
1,955,889	-		PP_1753	0.54	-0.8
1,955,938	-		PP_1753	0.59	+0.8
1,955,938	-		PP_1753	0.59	-0.2
1,955,952	-		PP_1753	0.60	-3.1
1,955,952	-		PP_1753	0.60	-0.7
1,956,065	+		PP_1753	0.72	+1.0
1,956,066	-		PP_1753	0.72	-0.2
1,956,185	+		PP_1753	0.84	+0.4
1,956,185	+		PP_1753	0.84	-0.5
1,956,185	+		PP_1753	0.84	-1.2
1,956,185	+		PP_1753	0.84	+1.3
1,956,185	+		PP_1753	0.84	+0.4
1,956,186	-		PP_1753	0.84	-0.4
1,956,186	-		PP_1753	0.84	+0.7
1,956,186	-		PP_1753	0.84	-0.5
1,956,186	-		PP_1753	0.84	-1.8
1,956,186	-		PP_1753	0.84	-1.6
1,956,218	+		PP_1753	0.88	-1.1
1,956,218	+		PP_1753	0.88	-0.0
1,956,218	+		PP_1753	0.88	-0.1
1,956,219	-		PP_1753	0.88	-0.2
1,956,219	-		PP_1753	0.88	-1.6
1,956,320	+				-1.0
1,956,321	-				-1.6
1,956,321	-				-0.5
1,956,371	-				+0.8
1,956,461	-				-1.5
1,956,524	+				-0.8
1,956,536	-		PP_1754	0.11	+0.2
1,956,598	+		PP_1754	0.16	-3.0
1,956,598	+		PP_1754	0.16	+0.5
1,956,599	-		PP_1754	0.16	-1.4
1,956,600	+		PP_1754	0.16	+0.5
1,956,600	+				+1.4
1,956,601	-		PP_1754	0.16	+2.5
1,956,694	+		PP_1754	0.24	-0.7
1,956,695	-		PP_1754	0.24	-1.5
1,956,702	+		PP_1754	0.25	+0.7
1,956,702	+		PP_1754	0.25	+1.0
1,956,702	+		PP_1754	0.25	-0.8
1,956,719	+		PP_1754	0.26	+1.3
1,956,753	+		PP_1754	0.29	-1.0
1,956,753	+		PP_1754	0.29	+0.8
1,956,753	+		PP_1754	0.29	-0.3
1,956,828	+		PP_1754	0.36	+1.0
1,956,902	+		PP_1754	0.42	+1.7
1,956,902	+		PP_1754	0.42	-0.6
1,956,940	-		PP_1754	0.45	-0.5
1,956,986	-				+3.4
1,956,986	-		PP_1754	0.49	+1.7
1,957,050	+		PP_1754	0.55	-0.4
1,957,098	-		PP_1754	0.59	+0.3
1,957,152	+		PP_1754	0.64	+1.6
1,957,159	+		PP_1754	0.64	-1.2
1,957,166	-		PP_1754	0.65	+1.5
1,957,216	+		PP_1754	0.69	+1.4
1,957,216	+		PP_1754	0.69	-0.7
1,957,216	+		PP_1754	0.69	-1.9
1,957,233	+		PP_1754	0.71	-1.1
1,957,233	+		PP_1754	0.71	+0.6
1,957,234	-		PP_1754	0.71	-0.2
1,957,276	+		PP_1754	0.74	-0.7
1,957,277	-		PP_1754	0.75	-0.9
1,957,371	+		PP_1754	0.83	+0.6
1,957,372	-		PP_1754	0.83	-1.4
1,957,423	+		PP_1754	0.87	-0.7
1,957,432	+		PP_1754	0.88	+0.8
1,957,432	+		PP_1754	0.88	-0.6
1,957,433	-		PP_1754	0.88	+0.1
1,957,513	+				-0.3
1,957,513	+				+0.8
1,957,514	-				+0.7
1,957,514	-				-1.8
1,957,540	+				-2.4
1,957,670	+				+1.4
1,957,670	+				+0.0
1,957,784	+				+0.9
1,957,784	+				+0.2
1,957,785	-				+0.3
1,957,785	-				+0.2
1,957,818	-		PP_1755	0.11	-1.9
1,957,824	+		PP_1755	0.11	-0.6
1,957,824	+		PP_1755	0.11	-1.5
1,957,824	+		PP_1755	0.11	+1.2
1,957,825	-		PP_1755	0.11	+0.9
1,957,825	-		PP_1755	0.11	+0.8
1,957,968	+		PP_1755	0.21	+0.3
1,958,050	+		PP_1755	0.27	-2.3
1,958,050	+		PP_1755	0.27	-0.6
1,958,050	+		PP_1755	0.27	-0.7
1,958,441	+		PP_1755	0.55	+0.8
1,958,442	-		PP_1755	0.55	+2.2
1,958,485	-		PP_1755	0.58	+0.2
1,958,524	+		PP_1755	0.61	+0.9