Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1748 and PP_1749 are separated by 169 nucleotides PP_1749 and PP_1750 are separated by 3 nucleotides
PP_1748: PP_1748 - conserved protein of unknown function, at 1,946,555 to 1,947,739
_1748
PP_1749: PP_1749 - GNAT family acetyltransferase, at 1,947,909 to 1,949,654
_1749
PP_1750: PP_1750 - asparagine synthetase, at 1,949,658 to 1,951,445
_1750
Position (kb)
1947
1948
1949
1950 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1946.919 kb on + strand, within PP_1748 at 1946.919 kb on + strand, within PP_1748 at 1946.926 kb on - strand, within PP_1748 at 1947.153 kb on - strand, within PP_1748 at 1947.283 kb on - strand, within PP_1748 at 1947.633 kb on + strand at 1947.634 kb on - strand at 1947.825 kb on + strand at 1947.848 kb on + strand at 1948.019 kb on - strand at 1948.214 kb on - strand, within PP_1749 at 1948.337 kb on + strand, within PP_1749 at 1948.829 kb on - strand, within PP_1749 at 1948.936 kb on + strand, within PP_1749 at 1948.985 kb on - strand, within PP_1749 at 1948.985 kb on - strand, within PP_1749 at 1949.183 kb on - strand, within PP_1749 at 1949.210 kb on + strand, within PP_1749 at 1949.252 kb on - strand, within PP_1749 at 1949.316 kb on + strand at 1949.425 kb on + strand, within PP_1749 at 1949.426 kb on - strand, within PP_1749 at 1949.426 kb on - strand, within PP_1749 at 1949.426 kb on - strand, within PP_1749 at 1949.506 kb on - strand at 1949.506 kb on - strand at 1949.632 kb on + strand at 1949.872 kb on + strand, within PP_1750 at 1949.872 kb on + strand, within PP_1750 at 1949.873 kb on - strand, within PP_1750 at 1949.873 kb on - strand, within PP_1750 at 1949.999 kb on - strand, within PP_1750 at 1950.208 kb on + strand, within PP_1750 at 1950.254 kb on - strand, within PP_1750 at 1950.254 kb on - strand, within PP_1750 at 1950.254 kb on - strand, within PP_1750 at 1950.254 kb on - strand, within PP_1750 at 1950.330 kb on + strand, within PP_1750 at 1950.331 kb on - strand, within PP_1750 at 1950.370 kb on + strand, within PP_1750 at 1950.370 kb on + strand, within PP_1750 at 1950.370 kb on + strand, within PP_1750 at 1950.371 kb on - strand, within PP_1750 at 1950.371 kb on - strand, within PP_1750 at 1950.371 kb on - strand, within PP_1750 at 1950.484 kb on + strand, within PP_1750 at 1950.484 kb on + strand, within PP_1750 at 1950.484 kb on + strand, within PP_1750
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,946,919 + PP_1748 0.31 -0.1 1,946,919 + PP_1748 0.31 +1.5 1,946,926 - PP_1748 0.31 +0.0 1,947,153 - PP_1748 0.50 +0.4 1,947,283 - PP_1748 0.61 -0.1 1,947,633 + -0.1 1,947,634 - -1.8 1,947,825 + -1.7 1,947,848 + -0.9 1,948,019 - +2.3 1,948,214 - PP_1749 0.17 -0.9 1,948,337 + PP_1749 0.25 -1.9 1,948,829 - PP_1749 0.53 +0.2 1,948,936 + PP_1749 0.59 -1.4 1,948,985 - PP_1749 0.62 -1.7 1,948,985 - PP_1749 0.62 -1.1 1,949,183 - PP_1749 0.73 +0.4 1,949,210 + PP_1749 0.75 -1.2 1,949,252 - PP_1749 0.77 -1.2 1,949,316 + +5.4 1,949,425 + PP_1749 0.87 +0.7 1,949,426 - PP_1749 0.87 -0.2 1,949,426 - PP_1749 0.87 -0.3 1,949,426 - PP_1749 0.87 +0.6 1,949,506 - -2.6 1,949,506 - +1.3 1,949,632 + -0.1 1,949,872 + PP_1750 0.12 -1.0 1,949,872 + PP_1750 0.12 -1.0 1,949,873 - PP_1750 0.12 -0.0 1,949,873 - PP_1750 0.12 -0.2 1,949,999 - PP_1750 0.19 -1.4 1,950,208 + PP_1750 0.31 -0.8 1,950,254 - PP_1750 0.33 +0.6 1,950,254 - PP_1750 0.33 +0.2 1,950,254 - PP_1750 0.33 +0.1 1,950,254 - PP_1750 0.33 +0.9 1,950,330 + PP_1750 0.38 +1.9 1,950,331 - PP_1750 0.38 -0.2 1,950,370 + PP_1750 0.40 -0.5 1,950,370 + PP_1750 0.40 +0.7 1,950,370 + PP_1750 0.40 -1.2 1,950,371 - PP_1750 0.40 +0.2 1,950,371 - PP_1750 0.40 -1.7 1,950,371 - PP_1750 0.40 +1.6 1,950,484 + PP_1750 0.46 +0.9 1,950,484 + PP_1750 0.46 +0.2 1,950,484 + PP_1750 0.46 +0.8
Or see this region's nucleotide sequence