Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1728 and PP_1729 are separated by 38 nucleotides PP_1729 and PP_1730 are separated by 104 nucleotides PP_1730 and PP_1731 are separated by 170 nucleotides PP_1731 and PP_1732 are separated by 149 nucleotides
PP_1728: PP_1728 - conserved membrane protein of unknown function, at 1,927,938 to 1,930,091
_1728
PP_1729: PP_1729 - conserved protein of unknown function, at 1,930,130 to 1,930,333
_1729
PP_1730: PP_1730 - Probable M18 family aminopeptidase 2, at 1,930,438 to 1,931,727
_1730
PP_1731: PP_1731 - 23S rRNA (746) and tRNA (32) pseudouridine synthase, at 1,931,898 to 1,932,533
_1731
PP_1732: PP_1732 - Cell division topological specificity factor, at 1,932,683 to 1,932,937
_1732
Position (kb)
1930
1931
1932 Strain fitness (log2 ratio)
-2
-1
0
1 at 1929.496 kb on + strand, within PP_1728 at 1929.497 kb on - strand, within PP_1728 at 1929.641 kb on - strand, within PP_1728 at 1930.030 kb on - strand at 1930.123 kb on + strand at 1930.123 kb on + strand at 1930.131 kb on + strand at 1930.131 kb on + strand at 1930.131 kb on + strand at 1930.132 kb on - strand at 1930.132 kb on - strand at 1930.177 kb on + strand, within PP_1729 at 1930.206 kb on - strand, within PP_1729 at 1930.434 kb on + strand at 1930.435 kb on - strand at 1930.435 kb on - strand at 1930.460 kb on + strand at 1930.460 kb on + strand at 1930.460 kb on + strand at 1930.474 kb on + strand at 1930.474 kb on + strand at 1930.475 kb on - strand at 1930.475 kb on - strand at 1930.645 kb on + strand, within PP_1730 at 1930.847 kb on + strand, within PP_1730 at 1930.848 kb on - strand, within PP_1730 at 1931.084 kb on + strand, within PP_1730 at 1931.084 kb on + strand, within PP_1730 at 1931.085 kb on - strand, within PP_1730 at 1931.402 kb on + strand, within PP_1730 at 1931.441 kb on - strand, within PP_1730 at 1931.570 kb on + strand, within PP_1730 at 1931.570 kb on + strand, within PP_1730 at 1931.571 kb on - strand, within PP_1730 at 1931.571 kb on - strand, within PP_1730 at 1931.571 kb on - strand, within PP_1730 at 1931.573 kb on + strand, within PP_1730 at 1931.574 kb on - strand, within PP_1730 at 1931.727 kb on + strand at 1931.728 kb on - strand at 1931.728 kb on - strand at 1931.818 kb on + strand at 1931.818 kb on + strand at 1931.818 kb on + strand at 1931.819 kb on - strand at 1931.864 kb on + strand at 1932.016 kb on + strand, within PP_1731 at 1932.134 kb on + strand, within PP_1731 at 1932.135 kb on - strand, within PP_1731 at 1932.135 kb on - strand, within PP_1731 at 1932.205 kb on + strand, within PP_1731 at 1932.206 kb on - strand, within PP_1731 at 1932.268 kb on + strand, within PP_1731 at 1932.268 kb on + strand, within PP_1731 at 1932.269 kb on - strand, within PP_1731 at 1932.269 kb on - strand, within PP_1731 at 1932.551 kb on + strand at 1932.552 kb on - strand at 1932.552 kb on - strand at 1932.552 kb on - strand at 1932.552 kb on - strand at 1932.603 kb on + strand at 1932.612 kb on + strand at 1932.613 kb on - strand at 1932.625 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,929,496 + PP_1728 0.72 -1.5 1,929,497 - PP_1728 0.72 +1.4 1,929,641 - PP_1728 0.79 +0.7 1,930,030 - -0.6 1,930,123 + -1.7 1,930,123 + -0.9 1,930,131 + -1.4 1,930,131 + -1.0 1,930,131 + +0.2 1,930,132 - -0.8 1,930,132 - +1.0 1,930,177 + PP_1729 0.23 -1.0 1,930,206 - PP_1729 0.37 -1.4 1,930,434 + +1.1 1,930,435 - -2.4 1,930,435 - +0.4 1,930,460 + -1.1 1,930,460 + +1.6 1,930,460 + -0.5 1,930,474 + -1.5 1,930,474 + -1.0 1,930,475 - +0.0 1,930,475 - -0.0 1,930,645 + PP_1730 0.16 -0.2 1,930,847 + PP_1730 0.32 -0.9 1,930,848 - PP_1730 0.32 -2.2 1,931,084 + PP_1730 0.50 -1.7 1,931,084 + PP_1730 0.50 -0.4 1,931,085 - PP_1730 0.50 +1.2 1,931,402 + PP_1730 0.75 -1.7 1,931,441 - PP_1730 0.78 -0.2 1,931,570 + PP_1730 0.88 +1.5 1,931,570 + PP_1730 0.88 -0.7 1,931,571 - PP_1730 0.88 -1.4 1,931,571 - PP_1730 0.88 -2.1 1,931,571 - PP_1730 0.88 -2.4 1,931,573 + PP_1730 0.88 +1.4 1,931,574 - PP_1730 0.88 -1.8 1,931,727 + -0.2 1,931,728 - -0.1 1,931,728 - -1.6 1,931,818 + -1.0 1,931,818 + -0.9 1,931,818 + -0.7 1,931,819 - -0.0 1,931,864 + -0.0 1,932,016 + PP_1731 0.19 -0.2 1,932,134 + PP_1731 0.37 +1.1 1,932,135 - PP_1731 0.37 +0.4 1,932,135 - PP_1731 0.37 +0.1 1,932,205 + PP_1731 0.48 +0.1 1,932,206 - PP_1731 0.48 +1.7 1,932,268 + PP_1731 0.58 +0.9 1,932,268 + PP_1731 0.58 +1.1 1,932,269 - PP_1731 0.58 +1.2 1,932,269 - PP_1731 0.58 +0.6 1,932,551 + +0.7 1,932,552 - -0.1 1,932,552 - +0.2 1,932,552 - -0.2 1,932,552 - +0.6 1,932,603 + -2.4 1,932,612 + +0.5 1,932,613 - -1.7 1,932,625 + -1.5
Or see this region's nucleotide sequence