Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1712 and PP_1713 are separated by 162 nucleotides PP_1713 and PP_1714 are separated by 253 nucleotides
PP_1712: PP_1712 - conserved protein of unknown function, at 1,912,062 to 1,913,471
_1712
PP_1713: PP_1713 - Transcriptional regulator PcaQ, at 1,913,634 to 1,914,566
_1713
PP_1714: PP_1714 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 1,914,820 to 1,915,572
_1714
Position (kb)
1913
1914
1915 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1912.634 kb on - strand, within PP_1712 at 1912.634 kb on - strand, within PP_1712 at 1912.769 kb on + strand, within PP_1712 at 1912.769 kb on + strand, within PP_1712 at 1912.770 kb on - strand, within PP_1712 at 1912.770 kb on - strand, within PP_1712 at 1912.770 kb on - strand, within PP_1712 at 1912.770 kb on - strand, within PP_1712 at 1912.907 kb on + strand, within PP_1712 at 1912.908 kb on - strand, within PP_1712 at 1912.909 kb on + strand, within PP_1712 at 1912.909 kb on + strand, within PP_1712 at 1912.909 kb on + strand, within PP_1712 at 1912.909 kb on + strand, within PP_1712 at 1912.909 kb on + strand, within PP_1712 at 1912.976 kb on + strand, within PP_1712 at 1913.031 kb on + strand, within PP_1712 at 1913.032 kb on - strand, within PP_1712 at 1913.032 kb on - strand, within PP_1712 at 1913.151 kb on + strand, within PP_1712 at 1913.151 kb on + strand, within PP_1712 at 1913.152 kb on - strand, within PP_1712 at 1913.152 kb on - strand, within PP_1712 at 1913.185 kb on + strand, within PP_1712 at 1913.185 kb on + strand, within PP_1712 at 1913.186 kb on - strand, within PP_1712 at 1913.186 kb on - strand, within PP_1712 at 1913.186 kb on - strand, within PP_1712 at 1913.284 kb on + strand, within PP_1712 at 1913.285 kb on - strand, within PP_1712 at 1913.310 kb on + strand, within PP_1712 at 1913.385 kb on + strand at 1913.385 kb on + strand at 1913.426 kb on + strand at 1913.426 kb on + strand at 1913.427 kb on - strand at 1913.427 kb on - strand at 1913.496 kb on - strand at 1913.496 kb on - strand at 1913.524 kb on - strand at 1913.569 kb on + strand at 1913.569 kb on + strand at 1913.569 kb on + strand at 1913.569 kb on + strand at 1913.569 kb on + strand at 1913.569 kb on + strand at 1913.570 kb on - strand at 1913.570 kb on - strand at 1913.570 kb on - strand at 1913.570 kb on - strand at 1913.570 kb on - strand at 1913.570 kb on - strand at 1913.570 kb on - strand at 1913.600 kb on - strand at 1913.611 kb on - strand at 1913.659 kb on - strand at 1914.135 kb on + strand, within PP_1713 at 1914.135 kb on + strand, within PP_1713 at 1914.346 kb on - strand at 1914.554 kb on + strand at 1914.554 kb on + strand at 1914.554 kb on + strand at 1914.555 kb on - strand at 1914.558 kb on + strand at 1914.559 kb on - strand at 1914.620 kb on - strand at 1914.663 kb on - strand at 1914.663 kb on - strand at 1914.701 kb on + strand at 1914.702 kb on - strand at 1914.702 kb on - strand at 1914.734 kb on + strand at 1914.766 kb on + strand at 1914.767 kb on - strand at 1914.767 kb on - strand at 1914.767 kb on - strand at 1914.767 kb on - strand at 1914.816 kb on + strand at 1914.816 kb on + strand at 1914.817 kb on - strand at 1914.817 kb on - strand at 1914.857 kb on + strand at 1914.931 kb on + strand, within PP_1714 at 1914.931 kb on + strand, within PP_1714 at 1914.932 kb on - strand, within PP_1714 at 1915.026 kb on + strand, within PP_1714 at 1915.064 kb on + strand, within PP_1714 at 1915.204 kb on + strand, within PP_1714 at 1915.205 kb on - strand, within PP_1714 at 1915.268 kb on - strand, within PP_1714 at 1915.399 kb on + strand, within PP_1714 at 1915.399 kb on + strand, within PP_1714 at 1915.429 kb on + strand, within PP_1714
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,912,634 - PP_1712 0.41 +0.1 1,912,634 - PP_1712 0.41 -0.8 1,912,769 + PP_1712 0.50 +1.5 1,912,769 + PP_1712 0.50 -0.9 1,912,770 - PP_1712 0.50 -1.2 1,912,770 - PP_1712 0.50 -0.1 1,912,770 - PP_1712 0.50 -0.7 1,912,770 - PP_1712 0.50 +1.0 1,912,907 + PP_1712 0.60 +0.1 1,912,908 - PP_1712 0.60 +0.8 1,912,909 + PP_1712 0.60 +0.1 1,912,909 + PP_1712 0.60 +0.5 1,912,909 + PP_1712 0.60 -1.5 1,912,909 + PP_1712 0.60 -0.5 1,912,909 + PP_1712 0.60 -0.4 1,912,976 + PP_1712 0.65 -2.2 1,913,031 + PP_1712 0.69 +0.6 1,913,032 - PP_1712 0.69 -1.3 1,913,032 - PP_1712 0.69 +2.1 1,913,151 + PP_1712 0.77 +1.5 1,913,151 + PP_1712 0.77 -0.1 1,913,152 - PP_1712 0.77 +0.6 1,913,152 - PP_1712 0.77 -1.9 1,913,185 + PP_1712 0.80 +0.1 1,913,185 + PP_1712 0.80 -0.3 1,913,186 - PP_1712 0.80 -0.3 1,913,186 - PP_1712 0.80 -0.1 1,913,186 - PP_1712 0.80 +0.9 1,913,284 + PP_1712 0.87 -2.1 1,913,285 - PP_1712 0.87 +0.8 1,913,310 + PP_1712 0.89 -0.1 1,913,385 + +0.1 1,913,385 + -0.8 1,913,426 + +0.6 1,913,426 + +0.1 1,913,427 - +1.1 1,913,427 - +1.9 1,913,496 - +0.4 1,913,496 - +0.7 1,913,524 - -0.4 1,913,569 + -0.5 1,913,569 + -0.0 1,913,569 + -0.8 1,913,569 + -2.5 1,913,569 + -2.3 1,913,569 + -1.1 1,913,570 - +0.2 1,913,570 - +2.5 1,913,570 - -1.7 1,913,570 - -0.2 1,913,570 - +0.9 1,913,570 - -0.6 1,913,570 - +2.5 1,913,600 - -1.5 1,913,611 - +2.0 1,913,659 - +0.6 1,914,135 + PP_1713 0.54 -2.9 1,914,135 + PP_1713 0.54 +0.3 1,914,346 - +2.4 1,914,554 + -0.9 1,914,554 + +0.8 1,914,554 + +0.3 1,914,555 - +0.4 1,914,558 + +0.9 1,914,559 - +0.3 1,914,620 - -0.0 1,914,663 - -1.2 1,914,663 - -0.0 1,914,701 + -0.9 1,914,702 - -0.9 1,914,702 - -2.6 1,914,734 + -0.3 1,914,766 + -1.1 1,914,767 - -0.3 1,914,767 - -1.6 1,914,767 - -1.2 1,914,767 - -0.3 1,914,816 + -1.6 1,914,816 + -0.9 1,914,817 - -1.5 1,914,817 - +1.0 1,914,857 + -0.2 1,914,931 + PP_1714 0.15 +0.0 1,914,931 + PP_1714 0.15 +0.9 1,914,932 - PP_1714 0.15 -0.0 1,915,026 + PP_1714 0.27 +2.1 1,915,064 + PP_1714 0.32 -1.9 1,915,204 + PP_1714 0.51 +1.3 1,915,205 - PP_1714 0.51 -1.1 1,915,268 - PP_1714 0.59 +0.6 1,915,399 + PP_1714 0.77 +0.1 1,915,399 + PP_1714 0.77 +0.4 1,915,429 + PP_1714 0.81 -1.0
Or see this region's nucleotide sequence