Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1549 and PP_1550 are separated by 88 nucleotides PP_1550 and PP_1551 are separated by 141 nucleotides
PP_1549: PP_1549 - Transcriptional regulator, Cro/CI family, at 1,749,384 to 1,750,235
_1549
PP_1550: PP_1550 - Transcriptional regulator, Cro/CI family, at 1,750,324 to 1,750,500
_1550
PP_1551: PP_1551 - putative Phage replication protein O, at 1,750,642 to 1,751,496
_1551
Position (kb)
1750
1751 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1749.324 kb on + strand at 1750.247 kb on - strand at 1750.247 kb on - strand at 1750.394 kb on - strand, within PP_1550 at 1750.394 kb on - strand, within PP_1550 at 1750.394 kb on - strand, within PP_1550 at 1750.394 kb on - strand, within PP_1550 at 1750.394 kb on - strand, within PP_1550 at 1750.394 kb on - strand, within PP_1550 at 1750.521 kb on - strand at 1750.521 kb on - strand at 1750.521 kb on - strand at 1750.539 kb on - strand at 1750.539 kb on - strand at 1750.619 kb on - strand at 1750.626 kb on + strand at 1750.627 kb on - strand at 1750.627 kb on - strand at 1750.627 kb on - strand at 1750.756 kb on + strand, within PP_1551 at 1750.756 kb on + strand, within PP_1551 at 1750.757 kb on - strand, within PP_1551 at 1750.948 kb on + strand, within PP_1551 at 1750.949 kb on - strand, within PP_1551 at 1751.044 kb on + strand, within PP_1551 at 1751.049 kb on + strand, within PP_1551 at 1751.049 kb on + strand, within PP_1551 at 1751.049 kb on + strand, within PP_1551 at 1751.065 kb on + strand, within PP_1551 at 1751.065 kb on + strand, within PP_1551 at 1751.065 kb on + strand, within PP_1551 at 1751.066 kb on - strand, within PP_1551 at 1751.069 kb on + strand, within PP_1551 at 1751.069 kb on + strand, within PP_1551 at 1751.069 kb on + strand, within PP_1551 at 1751.069 kb on + strand, within PP_1551 at 1751.070 kb on - strand, within PP_1551 at 1751.091 kb on + strand, within PP_1551 at 1751.091 kb on + strand, within PP_1551 at 1751.091 kb on + strand, within PP_1551 at 1751.091 kb on + strand, within PP_1551 at 1751.092 kb on - strand, within PP_1551 at 1751.092 kb on - strand, within PP_1551 at 1751.092 kb on - strand, within PP_1551 at 1751.092 kb on - strand, within PP_1551 at 1751.092 kb on - strand, within PP_1551 at 1751.092 kb on - strand, within PP_1551 at 1751.212 kb on + strand, within PP_1551 at 1751.486 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,749,324 + +0.6 1,750,247 - -1.4 1,750,247 - +0.1 1,750,394 - PP_1550 0.40 -0.4 1,750,394 - PP_1550 0.40 +0.5 1,750,394 - PP_1550 0.40 +1.5 1,750,394 - PP_1550 0.40 +0.8 1,750,394 - PP_1550 0.40 -0.3 1,750,394 - PP_1550 0.40 +1.5 1,750,521 - +0.4 1,750,521 - -2.4 1,750,521 - +0.4 1,750,539 - -0.9 1,750,539 - +1.8 1,750,619 - -1.0 1,750,626 + -3.4 1,750,627 - -2.0 1,750,627 - +2.7 1,750,627 - -0.0 1,750,756 + PP_1551 0.13 -1.7 1,750,756 + PP_1551 0.13 +0.9 1,750,757 - PP_1551 0.13 +0.5 1,750,948 + PP_1551 0.36 +0.6 1,750,949 - PP_1551 0.36 +0.7 1,751,044 + PP_1551 0.47 +0.9 1,751,049 + PP_1551 0.48 -0.7 1,751,049 + PP_1551 0.48 -1.3 1,751,049 + PP_1551 0.48 +0.3 1,751,065 + PP_1551 0.49 +0.6 1,751,065 + PP_1551 0.49 -2.6 1,751,065 + PP_1551 0.49 +0.6 1,751,066 - PP_1551 0.50 +0.2 1,751,069 + PP_1551 0.50 +0.9 1,751,069 + PP_1551 0.50 +0.3 1,751,069 + PP_1551 0.50 +0.0 1,751,069 + PP_1551 0.50 +0.2 1,751,070 - PP_1551 0.50 +1.4 1,751,091 + PP_1551 0.53 +0.4 1,751,091 + PP_1551 0.53 -1.4 1,751,091 + PP_1551 0.53 -1.3 1,751,091 + PP_1551 0.53 -0.7 1,751,092 - PP_1551 0.53 +1.2 1,751,092 - PP_1551 0.53 +1.6 1,751,092 - PP_1551 0.53 +0.6 1,751,092 - PP_1551 0.53 -0.4 1,751,092 - PP_1551 0.53 +1.0 1,751,092 - PP_1551 0.53 +1.8 1,751,212 + PP_1551 0.67 +0.2 1,751,486 - +0.3
Or see this region's nucleotide sequence