Strain Fitness in Pseudomonas putida KT2440 around PP_1533

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
1,738,194	+				-1.9
1,738,194	+				+1.4
1,738,194	+				-0.3
1,738,194	+				+2.0
1,738,441	-		PP_1532	0.32	+1.4
1,738,554	+		PP_1532	0.42	-1.3
1,738,555	-		PP_1532	0.42	-2.2
1,738,556	+		PP_1532	0.42	+1.5
1,738,556	+		PP_1532	0.42	+1.3
1,738,556	+		PP_1532	0.42	+1.0
1,738,556	+		PP_1532	0.42	+0.9
1,738,557	-		PP_1532	0.43	-0.7
1,738,557	-		PP_1532	0.43	-0.4
1,738,606	+		PP_1532	0.47	-0.9
1,738,607	-		PP_1532	0.47	-1.2
1,738,607	-		PP_1532	0.47	+0.8
1,738,626	+		PP_1532	0.49	+0.4
1,738,627	-		PP_1532	0.49	+0.1
1,738,627	-		PP_1532	0.49	-0.6
1,738,670	+		PP_1532	0.53	-1.0
1,738,670	+		PP_1532	0.53	-1.0
1,738,688	-		PP_1532	0.54	+3.8
1,738,735	-		PP_1532	0.59	-0.0
1,738,745	+		PP_1532	0.60	+0.8
1,738,780	+		PP_1532	0.63	+0.1
1,738,788	+		PP_1532	0.63	-0.6
1,738,789	-		PP_1532	0.64	-1.3
1,738,792	+		PP_1532	0.64	+0.2
1,738,792	+		PP_1532	0.64	-0.4
1,738,793	-		PP_1532	0.64	-1.1
1,738,868	+		PP_1532	0.71	-0.8
1,738,924	+		PP_1532	0.76	+2.4
1,738,924	+		PP_1532	0.76	-0.6
1,738,924	+		PP_1532	0.76	+0.9
1,739,099	-				-1.8
1,739,099	-				-1.3
1,739,104	+				-0.4
1,739,104	+				-0.7
1,739,105	-				+0.2
1,739,107	+				-1.5
1,739,107	+				-0.3
1,739,107	+				+1.8
1,739,107	+				-0.8
1,739,108	-				+0.9
1,739,108	-				-0.1
1,739,108	-				+0.4
1,739,108	-				-1.1
1,739,108	-				-0.4
1,739,120	+				+1.3
1,739,141	-				+0.4
1,739,155	-				+0.4
1,739,214	+		PP_1533	0.17	+0.9
1,739,285	+		PP_1533	0.46	+0.2
1,739,285	+		PP_1533	0.46	-0.9
1,739,285	+		PP_1533	0.46	+1.6
1,739,285	+		PP_1533	0.46	-0.0
1,739,285	+		PP_1533	0.46	-0.6
1,739,285	+		PP_1533	0.46	-0.7
1,739,285	+		PP_1533	0.46	-1.9
1,739,285	+		PP_1533	0.46	-1.6
1,739,285	+		PP_1533	0.46	-0.0
1,739,286	-		PP_1533	0.47	+0.2
1,739,286	-		PP_1533	0.47	-1.7
1,739,286	-		PP_1533	0.47	-0.2
1,739,286	-		PP_1533	0.47	-0.7
1,739,286	-		PP_1533	0.47	+1.0
1,739,286	-		PP_1533	0.47	-0.4
1,739,288	+		PP_1533	0.47	+1.2
1,739,288	+		PP_1533	0.47	-0.5
1,739,288	+		PP_1533	0.47	-1.5
1,739,288	+		PP_1533	0.47	-0.5
1,739,288	+		PP_1533	0.47	-0.5
1,739,288	+		PP_1533	0.47	+1.1
1,739,289	-		PP_1533	0.48	-0.9
1,739,289	-		PP_1533	0.48	-0.3
1,739,289	-		PP_1533	0.48	-1.2
1,739,289	-		PP_1533	0.48	-1.4
1,739,305	+		PP_1533	0.54	-1.4
1,739,458	-				-2.8
1,739,464	+				-0.3
1,739,464	+				+0.1
1,739,465	-				+1.7
1,739,558	+		PP_1534	0.34	-1.3
1,739,558	+		PP_1534	0.34	+0.4
1,739,656	+		PP_1534	0.65	+2.1
1,739,656	+		PP_1534	0.65	-1.7
1,739,656	+		PP_1534	0.65	-2.6
1,739,672	+		PP_1534	0.70	-0.7
1,739,672	+		PP_1534	0.70	+0.3
1,739,672	+		PP_1534	0.70	+1.5
1,739,772	+				-0.3
1,739,772	+				-0.7
1,739,772	+				-1.1
1,739,772	+				+0.1
1,739,772	+				-1.7
1,739,773	-				+1.1
1,739,811	+				-1.1
1,739,931	+		PP_1535	0.23	+0.3
1,739,931	+		PP_1535	0.23	-0.4
1,739,931	+		PP_1535	0.23	-2.2
1,739,932	-		PP_1535	0.23	-1.4
1,739,932	-		PP_1535	0.23	-2.1
1,739,932	-		PP_1535	0.23	-1.6
1,740,046	+		PP_1535	0.48	+0.5