Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1511 and PP_1512 are separated by 2 nucleotides PP_1512 and PP_1513 are separated by 104 nucleotides PP_1513 and PP_1514 are separated by 192 nucleotides
PP_1511: PP_1511 - conserved protein of unknown function, at 1,715,885 to 1,716,574
_1511
PP_1512: PP_1512 - conserved protein of unknown function, at 1,716,577 to 1,717,197
_1512
PP_1513: PP_1513 - Ribosomal RNA small subunit methyltransferase J, at 1,717,302 to 1,718,078
_1513
PP_1514: PP_1514 - conserved protein of unknown function, at 1,718,271 to 1,719,239
_1514
Position (kb)
1717
1718
1719 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1716.320 kb on + strand, within PP_1511 at 1716.512 kb on + strand at 1717.023 kb on - strand, within PP_1512 at 1717.023 kb on - strand, within PP_1512 at 1717.023 kb on - strand, within PP_1512 at 1717.091 kb on - strand, within PP_1512 at 1717.145 kb on + strand at 1717.146 kb on - strand at 1717.166 kb on + strand at 1717.166 kb on + strand at 1717.167 kb on - strand at 1717.167 kb on - strand at 1717.167 kb on - strand at 1717.188 kb on + strand at 1717.189 kb on - strand at 1717.189 kb on - strand at 1717.189 kb on - strand at 1717.270 kb on - strand at 1717.351 kb on - strand at 1717.351 kb on - strand at 1717.594 kb on + strand, within PP_1513 at 1717.825 kb on - strand, within PP_1513 at 1717.825 kb on - strand, within PP_1513 at 1718.019 kb on - strand at 1718.031 kb on - strand at 1718.044 kb on - strand at 1718.052 kb on + strand at 1718.052 kb on + strand at 1718.053 kb on - strand at 1718.087 kb on + strand at 1718.088 kb on - strand at 1718.110 kb on + strand at 1718.110 kb on + strand at 1718.110 kb on + strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.111 kb on - strand at 1718.176 kb on + strand at 1718.188 kb on + strand at 1718.188 kb on + strand at 1718.189 kb on - strand at 1718.189 kb on - strand at 1718.202 kb on - strand at 1718.202 kb on - strand at 1718.214 kb on - strand at 1718.214 kb on - strand at 1718.220 kb on + strand at 1718.220 kb on + strand at 1718.255 kb on - strand at 1718.255 kb on - strand at 1718.270 kb on - strand at 1718.292 kb on + strand at 1718.292 kb on + strand at 1718.339 kb on - strand at 1718.339 kb on - strand at 1718.339 kb on - strand at 1718.479 kb on - strand, within PP_1514 at 1718.508 kb on + strand, within PP_1514 at 1718.508 kb on + strand, within PP_1514 at 1718.509 kb on - strand, within PP_1514 at 1718.509 kb on - strand, within PP_1514 at 1718.598 kb on + strand, within PP_1514 at 1718.598 kb on + strand, within PP_1514 at 1718.599 kb on - strand, within PP_1514 at 1718.599 kb on - strand, within PP_1514 at 1718.616 kb on + strand, within PP_1514 at 1718.617 kb on - strand, within PP_1514 at 1718.617 kb on - strand, within PP_1514 at 1718.697 kb on + strand, within PP_1514 at 1718.698 kb on - strand at 1718.698 kb on - strand, within PP_1514 at 1718.698 kb on - strand, within PP_1514 at 1718.698 kb on - strand, within PP_1514 at 1718.703 kb on + strand, within PP_1514 at 1718.703 kb on + strand, within PP_1514 at 1718.756 kb on + strand, within PP_1514 at 1718.809 kb on + strand, within PP_1514 at 1718.820 kb on - strand, within PP_1514 at 1718.820 kb on - strand, within PP_1514 at 1718.828 kb on - strand, within PP_1514 at 1718.870 kb on + strand, within PP_1514 at 1718.871 kb on - strand, within PP_1514 at 1718.876 kb on - strand, within PP_1514 at 1718.885 kb on + strand, within PP_1514 at 1718.885 kb on + strand, within PP_1514 at 1718.923 kb on + strand, within PP_1514 at 1718.924 kb on - strand, within PP_1514 at 1718.972 kb on + strand, within PP_1514
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,716,320 + PP_1511 0.63 +0.1 1,716,512 + -1.8 1,717,023 - PP_1512 0.72 +0.3 1,717,023 - PP_1512 0.72 -0.3 1,717,023 - PP_1512 0.72 -0.7 1,717,091 - PP_1512 0.83 -0.3 1,717,145 + -1.4 1,717,146 - -1.4 1,717,166 + -0.8 1,717,166 + -0.6 1,717,167 - +0.2 1,717,167 - -2.2 1,717,167 - -0.9 1,717,188 + +0.9 1,717,189 - -1.7 1,717,189 - +0.3 1,717,189 - +0.7 1,717,270 - -0.3 1,717,351 - -1.9 1,717,351 - -2.0 1,717,594 + PP_1513 0.38 -0.1 1,717,825 - PP_1513 0.67 -2.8 1,717,825 - PP_1513 0.67 -1.0 1,718,019 - +0.0 1,718,031 - +0.0 1,718,044 - -2.1 1,718,052 + -0.4 1,718,052 + -2.2 1,718,053 - +0.3 1,718,087 + -0.3 1,718,088 - +0.3 1,718,110 + -0.3 1,718,110 + +0.1 1,718,110 + +2.8 1,718,111 - -1.7 1,718,111 - -0.3 1,718,111 - +0.7 1,718,111 - +1.1 1,718,176 + -0.5 1,718,188 + +0.6 1,718,188 + -1.0 1,718,189 - -0.3 1,718,189 - -1.2 1,718,202 - +0.4 1,718,202 - -0.1 1,718,214 - -1.4 1,718,214 - -0.2 1,718,220 + +0.6 1,718,220 + -0.4 1,718,255 - +0.4 1,718,255 - +0.4 1,718,270 - +0.0 1,718,292 + +0.2 1,718,292 + -0.8 1,718,339 - +0.6 1,718,339 - +0.1 1,718,339 - +0.5 1,718,479 - PP_1514 0.21 +0.0 1,718,508 + PP_1514 0.24 +0.1 1,718,508 + PP_1514 0.24 +0.9 1,718,509 - PP_1514 0.25 -1.3 1,718,509 - PP_1514 0.25 -1.1 1,718,598 + PP_1514 0.34 +0.9 1,718,598 + PP_1514 0.34 +0.2 1,718,599 - PP_1514 0.34 +0.3 1,718,599 - PP_1514 0.34 +1.3 1,718,616 + PP_1514 0.36 +0.4 1,718,617 - PP_1514 0.36 +0.3 1,718,617 - PP_1514 0.36 +0.0 1,718,697 + PP_1514 0.44 -0.2 1,718,698 - +2.0 1,718,698 - PP_1514 0.44 +0.9 1,718,698 - PP_1514 0.44 +1.1 1,718,698 - PP_1514 0.44 -1.2 1,718,703 + PP_1514 0.45 -0.4 1,718,703 + PP_1514 0.45 -0.4 1,718,756 + PP_1514 0.50 -1.2 1,718,809 + PP_1514 0.56 -1.7 1,718,820 - PP_1514 0.57 +2.9 1,718,820 - PP_1514 0.57 -0.9 1,718,828 - PP_1514 0.57 -0.6 1,718,870 + PP_1514 0.62 -0.0 1,718,871 - PP_1514 0.62 -0.2 1,718,876 - PP_1514 0.62 -0.5 1,718,885 + PP_1514 0.63 +0.4 1,718,885 + PP_1514 0.63 -1.0 1,718,923 + PP_1514 0.67 +0.8 1,718,924 - PP_1514 0.67 -2.7 1,718,972 + PP_1514 0.72 -1.3
Or see this region's nucleotide sequence