Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1464 and PP_1465 are separated by 42 nucleotides PP_1465 and PP_1466 are separated by 94 nucleotides PP_1466 and PP_1467 are separated by 3 nucleotides PP_1467 and PP_1468 are separated by 68 nucleotides
PP_1464: PP_1464 - tRNA (guanine-N(1)-)-methyltransferase, at 1,669,432 to 1,670,184
_1464
PP_1465: PP_1465 - 50S ribosomal protein L19, at 1,670,227 to 1,670,577
_1465
PP_1466: PP_1466 - Long-chain acyl-CoA thioester hydrolase family protein, at 1,670,672 to 1,671,079
_1466
PP_1467: PP_1467 - NhaP-type Na+(K+)/H+ antiporter, at 1,671,083 to 1,672,888
_1467
PP_1468: PP_1468 - Tyrosine recombinase XerD, at 1,672,957 to 1,673,853
_1468
Position (kb)
1671
1672
1673 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1670.750 kb on - strand, within PP_1466 at 1670.942 kb on - strand, within PP_1466 at 1670.942 kb on - strand, within PP_1466 at 1670.984 kb on + strand, within PP_1466 at 1670.985 kb on - strand, within PP_1466 at 1670.985 kb on - strand, within PP_1466 at 1671.081 kb on + strand at 1671.082 kb on - strand at 1671.148 kb on + strand at 1671.161 kb on + strand at 1671.162 kb on - strand at 1671.162 kb on - strand at 1671.162 kb on - strand at 1671.228 kb on + strand at 1671.228 kb on + strand at 1671.228 kb on + strand at 1671.265 kb on + strand, within PP_1467 at 1671.265 kb on + strand, within PP_1467 at 1671.265 kb on + strand, within PP_1467 at 1671.265 kb on + strand, within PP_1467 at 1671.266 kb on - strand, within PP_1467 at 1671.267 kb on + strand, within PP_1467 at 1671.268 kb on - strand, within PP_1467 at 1671.310 kb on - strand, within PP_1467 at 1671.310 kb on - strand, within PP_1467 at 1671.310 kb on - strand, within PP_1467 at 1671.332 kb on + strand, within PP_1467 at 1671.332 kb on + strand, within PP_1467 at 1671.333 kb on - strand, within PP_1467 at 1671.373 kb on + strand, within PP_1467 at 1671.373 kb on + strand, within PP_1467 at 1671.373 kb on + strand, within PP_1467 at 1671.458 kb on + strand, within PP_1467 at 1671.549 kb on + strand, within PP_1467 at 1672.001 kb on + strand, within PP_1467 at 1672.002 kb on - strand, within PP_1467 at 1672.191 kb on - strand, within PP_1467 at 1672.230 kb on + strand, within PP_1467 at 1672.230 kb on + strand, within PP_1467 at 1672.231 kb on - strand, within PP_1467 at 1672.231 kb on - strand, within PP_1467 at 1672.348 kb on + strand, within PP_1467 at 1672.377 kb on + strand, within PP_1467 at 1672.900 kb on - strand at 1672.900 kb on - strand at 1672.900 kb on - strand at 1672.914 kb on - strand at 1672.923 kb on + strand at 1672.924 kb on - strand at 1672.924 kb on - strand at 1672.924 kb on - strand at 1673.003 kb on + strand at 1673.158 kb on + strand, within PP_1468 at 1673.387 kb on - strand, within PP_1468 at 1673.533 kb on + strand, within PP_1468 at 1673.637 kb on + strand, within PP_1468 at 1673.791 kb on + strand at 1673.791 kb on + strand at 1673.791 kb on + strand at 1673.791 kb on + strand at 1673.791 kb on + strand at 1673.791 kb on + strand at 1673.792 kb on - strand at 1673.792 kb on - strand at 1673.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,670,750 - PP_1466 0.19 -1.1 1,670,942 - PP_1466 0.66 +0.7 1,670,942 - PP_1466 0.66 +0.5 1,670,984 + PP_1466 0.76 -0.8 1,670,985 - PP_1466 0.77 -0.1 1,670,985 - PP_1466 0.77 -0.5 1,671,081 + -0.4 1,671,082 - -1.4 1,671,148 + +0.4 1,671,161 + -0.4 1,671,162 - +0.3 1,671,162 - +1.1 1,671,162 - +0.7 1,671,228 + -0.3 1,671,228 + +0.4 1,671,228 + +0.6 1,671,265 + PP_1467 0.10 -0.3 1,671,265 + PP_1467 0.10 +1.4 1,671,265 + PP_1467 0.10 -1.1 1,671,265 + PP_1467 0.10 -1.4 1,671,266 - PP_1467 0.10 +0.2 1,671,267 + PP_1467 0.10 +0.7 1,671,268 - PP_1467 0.10 -0.1 1,671,310 - PP_1467 0.13 -0.9 1,671,310 - PP_1467 0.13 +0.7 1,671,310 - PP_1467 0.13 -1.7 1,671,332 + PP_1467 0.14 -1.0 1,671,332 + PP_1467 0.14 -0.5 1,671,333 - PP_1467 0.14 -1.5 1,671,373 + PP_1467 0.16 -0.9 1,671,373 + PP_1467 0.16 +1.3 1,671,373 + PP_1467 0.16 +1.7 1,671,458 + PP_1467 0.21 +1.9 1,671,549 + PP_1467 0.26 +0.2 1,672,001 + PP_1467 0.51 +1.7 1,672,002 - PP_1467 0.51 -0.4 1,672,191 - PP_1467 0.61 +0.1 1,672,230 + PP_1467 0.64 +1.5 1,672,230 + PP_1467 0.64 +0.8 1,672,231 - PP_1467 0.64 -1.8 1,672,231 - PP_1467 0.64 +0.9 1,672,348 + PP_1467 0.70 +0.4 1,672,377 + PP_1467 0.72 -0.2 1,672,900 - -0.2 1,672,900 - -0.4 1,672,900 - -2.0 1,672,914 - -0.1 1,672,923 + +0.6 1,672,924 - -0.5 1,672,924 - -2.3 1,672,924 - -0.0 1,673,003 + -2.4 1,673,158 + PP_1468 0.22 +0.3 1,673,387 - PP_1468 0.48 -1.9 1,673,533 + PP_1468 0.64 -0.3 1,673,637 + PP_1468 0.76 -0.8 1,673,791 + +0.5 1,673,791 + -0.7 1,673,791 + -1.2 1,673,791 + -0.3 1,673,791 + +0.8 1,673,791 + -0.1 1,673,792 - -2.4 1,673,792 - -0.6 1,673,794 - -1.7
Or see this region's nucleotide sequence