Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1365 and PP_1366 are separated by 73 nucleotides PP_1366 and PP_1367 are separated by 283 nucleotides
PP_1365: PP_1365 - exodeoxyribonuclease I, at 1,553,929 to 1,555,362
_1365
PP_1366: PP_1366 - Transcriptional regulator MvaT, P16 subunit, at 1,555,436 to 1,555,813
_1366
PP_1367: PP_1367 - formyltetrahydrofolate hydrolase, at 1,556,097 to 1,556,948
_1367
Position (kb)
1555
1556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1555.349 kb on - strand at 1555.349 kb on - strand at 1555.370 kb on + strand at 1555.371 kb on - strand at 1555.425 kb on + strand at 1555.557 kb on + strand at 1555.592 kb on - strand, within PP_1366 at 1555.592 kb on - strand, within PP_1366 at 1555.854 kb on + strand at 1555.855 kb on - strand at 1555.857 kb on + strand at 1555.857 kb on + strand at 1555.858 kb on - strand at 1555.868 kb on + strand at 1555.869 kb on - strand at 1555.885 kb on - strand at 1555.885 kb on - strand at 1555.899 kb on - strand at 1556.011 kb on + strand at 1556.011 kb on + strand at 1556.011 kb on + strand at 1556.011 kb on + strand at 1556.012 kb on - strand at 1556.012 kb on - strand at 1556.034 kb on + strand at 1556.064 kb on + strand at 1556.064 kb on + strand at 1556.064 kb on + strand at 1556.106 kb on + strand at 1556.106 kb on + strand at 1556.107 kb on - strand at 1556.137 kb on + strand at 1556.138 kb on - strand at 1556.138 kb on - strand at 1556.138 kb on - strand at 1556.175 kb on + strand at 1556.175 kb on + strand at 1556.175 kb on + strand at 1556.176 kb on - strand at 1556.176 kb on - strand at 1556.176 kb on - strand at 1556.365 kb on + strand, within PP_1367 at 1556.366 kb on - strand, within PP_1367 at 1556.366 kb on - strand, within PP_1367 at 1556.516 kb on - strand, within PP_1367 at 1556.516 kb on - strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.607 kb on + strand, within PP_1367 at 1556.608 kb on - strand, within PP_1367 at 1556.643 kb on + strand, within PP_1367 at 1556.643 kb on + strand, within PP_1367 at 1556.644 kb on - strand, within PP_1367 at 1556.706 kb on + strand, within PP_1367 at 1556.707 kb on - strand, within PP_1367 at 1556.757 kb on + strand, within PP_1367 at 1556.758 kb on - strand, within PP_1367 at 1556.758 kb on - strand, within PP_1367
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,555,349 - +1.1 1,555,349 - -0.2 1,555,370 + -0.4 1,555,371 - -0.2 1,555,425 + +1.3 1,555,557 + +3.8 1,555,592 - PP_1366 0.41 +1.9 1,555,592 - PP_1366 0.41 +0.4 1,555,854 + +0.2 1,555,855 - +1.0 1,555,857 + +1.8 1,555,857 + -0.0 1,555,858 - -1.5 1,555,868 + -0.6 1,555,869 - +0.6 1,555,885 - -2.2 1,555,885 - -0.3 1,555,899 - -1.3 1,556,011 + +1.3 1,556,011 + +0.4 1,556,011 + -0.1 1,556,011 + -0.8 1,556,012 - -1.9 1,556,012 - -1.1 1,556,034 + -1.1 1,556,064 + +0.1 1,556,064 + +1.8 1,556,064 + +1.8 1,556,106 + -0.5 1,556,106 + -0.6 1,556,107 - +1.2 1,556,137 + +1.8 1,556,138 - +0.1 1,556,138 - -1.0 1,556,138 - +0.8 1,556,175 + +1.2 1,556,175 + +0.2 1,556,175 + -0.4 1,556,176 - +0.9 1,556,176 - +0.7 1,556,176 - +0.2 1,556,365 + PP_1367 0.31 +1.0 1,556,366 - PP_1367 0.32 -0.9 1,556,366 - PP_1367 0.32 +0.0 1,556,516 - PP_1367 0.49 +0.6 1,556,516 - PP_1367 0.49 +1.8 1,556,607 + PP_1367 0.60 -0.3 1,556,607 + PP_1367 0.60 -0.1 1,556,607 + PP_1367 0.60 +0.2 1,556,607 + PP_1367 0.60 -0.5 1,556,608 - PP_1367 0.60 +0.9 1,556,643 + PP_1367 0.64 +3.8 1,556,643 + PP_1367 0.64 -0.1 1,556,644 - PP_1367 0.64 +0.9 1,556,706 + PP_1367 0.71 +1.4 1,556,707 - PP_1367 0.72 +0.7 1,556,757 + PP_1367 0.77 -0.9 1,556,758 - PP_1367 0.78 +0.2 1,556,758 - PP_1367 0.78 +0.1
Or see this region's nucleotide sequence