Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1319 and PP_1320 are separated by 116 nucleotides PP_1320 and PP_1321 overlap by 20 nucleotides PP_1321 and PP_1322 are separated by 61 nucleotides PP_1322 and PP_1323 overlap by 4 nucleotides
PP_1319: PP_1319 - Ubiquinol--cytochrome c reductase, cytochrome c1, at 1,506,100 to 1,506,879
_1319
PP_1320: PP_1320 - stringent starvation protein A, at 1,506,996 to 1,507,619
_1320
PP_1321: PP_1321 - ClpXP protease specificity-enhancing factor, at 1,507,600 to 1,508,061
_1321
PP_1322: PP_1322 - putative Lipoprotein, at 1,508,123 to 1,508,701
_1322
PP_1323: PP_1323 - factor modulating bacterial replication archosome assembly, at 1,508,698 to 1,509,285
_1323
Position (kb)
1507
1508
1509 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1506.758 kb on - strand, within PP_1319 at 1506.802 kb on + strand at 1506.802 kb on + strand at 1506.802 kb on + strand at 1506.802 kb on + strand at 1506.802 kb on + strand at 1506.802 kb on + strand at 1506.802 kb on + strand at 1506.803 kb on - strand at 1506.803 kb on - strand at 1506.803 kb on - strand at 1506.803 kb on - strand at 1506.803 kb on - strand at 1506.814 kb on + strand at 1506.815 kb on - strand at 1506.815 kb on - strand at 1506.815 kb on - strand at 1506.815 kb on - strand at 1506.815 kb on - strand at 1506.842 kb on - strand at 1506.860 kb on - strand at 1506.860 kb on - strand at 1506.860 kb on - strand at 1506.888 kb on + strand at 1506.888 kb on + strand at 1506.922 kb on + strand at 1506.922 kb on + strand at 1506.922 kb on + strand at 1506.922 kb on + strand at 1506.928 kb on + strand at 1506.928 kb on + strand at 1506.929 kb on - strand at 1506.929 kb on - strand at 1506.994 kb on - strand at 1507.015 kb on + strand at 1507.023 kb on + strand at 1507.024 kb on - strand at 1507.024 kb on - strand at 1507.044 kb on + strand at 1507.044 kb on + strand at 1507.149 kb on + strand, within PP_1320 at 1507.150 kb on - strand, within PP_1320 at 1507.150 kb on - strand, within PP_1320 at 1507.191 kb on + strand, within PP_1320 at 1507.191 kb on + strand, within PP_1320 at 1507.216 kb on - strand, within PP_1320 at 1507.216 kb on - strand, within PP_1320 at 1507.216 kb on - strand, within PP_1320 at 1507.256 kb on - strand, within PP_1320 at 1507.257 kb on + strand, within PP_1320 at 1507.346 kb on + strand, within PP_1320 at 1507.472 kb on - strand, within PP_1320 at 1507.480 kb on + strand, within PP_1320 at 1507.543 kb on - strand, within PP_1320 at 1507.543 kb on - strand, within PP_1320 at 1507.617 kb on + strand at 1507.618 kb on - strand at 1507.618 kb on - strand at 1507.649 kb on - strand, within PP_1321 at 1507.666 kb on + strand, within PP_1321 at 1507.667 kb on - strand, within PP_1321 at 1507.667 kb on - strand, within PP_1321 at 1507.701 kb on + strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.702 kb on - strand, within PP_1321 at 1507.776 kb on + strand, within PP_1321 at 1507.776 kb on + strand, within PP_1321 at 1507.776 kb on + strand, within PP_1321 at 1507.777 kb on - strand, within PP_1321 at 1507.777 kb on - strand, within PP_1321 at 1507.894 kb on + strand, within PP_1321 at 1507.894 kb on + strand, within PP_1321 at 1507.894 kb on + strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.895 kb on - strand, within PP_1321 at 1507.918 kb on - strand, within PP_1321 at 1508.059 kb on + strand at 1508.060 kb on - strand at 1508.133 kb on + strand at 1508.475 kb on + strand, within PP_1322 at 1508.476 kb on - strand, within PP_1322 at 1508.692 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,506,758 - PP_1319 0.84 +0.7 1,506,802 + +0.7 1,506,802 + -0.1 1,506,802 + +1.1 1,506,802 + -0.8 1,506,802 + +0.8 1,506,802 + +1.1 1,506,802 + +1.8 1,506,803 - +1.5 1,506,803 - +0.6 1,506,803 - +1.3 1,506,803 - -0.4 1,506,803 - +0.0 1,506,814 + -1.3 1,506,815 - +1.4 1,506,815 - +0.9 1,506,815 - +2.4 1,506,815 - +1.1 1,506,815 - -0.5 1,506,842 - -0.3 1,506,860 - +0.1 1,506,860 - -0.8 1,506,860 - +0.1 1,506,888 + +0.7 1,506,888 + +0.5 1,506,922 + -1.2 1,506,922 + -0.5 1,506,922 + -0.3 1,506,922 + -1.0 1,506,928 + +0.5 1,506,928 + +0.5 1,506,929 - +1.1 1,506,929 - +0.6 1,506,994 - +0.3 1,507,015 + -0.2 1,507,023 + +1.2 1,507,024 - +0.4 1,507,024 - +1.0 1,507,044 + -0.6 1,507,044 + +0.8 1,507,149 + PP_1320 0.25 -2.0 1,507,150 - PP_1320 0.25 -2.1 1,507,150 - PP_1320 0.25 -0.3 1,507,191 + PP_1320 0.31 -0.3 1,507,191 + PP_1320 0.31 -0.8 1,507,216 - PP_1320 0.35 -1.0 1,507,216 - PP_1320 0.35 +1.8 1,507,216 - PP_1320 0.35 +0.9 1,507,256 - PP_1320 0.42 +0.2 1,507,257 + PP_1320 0.42 -0.1 1,507,346 + PP_1320 0.56 +2.1 1,507,472 - PP_1320 0.76 -0.4 1,507,480 + PP_1320 0.78 +0.2 1,507,543 - PP_1320 0.88 -1.2 1,507,543 - PP_1320 0.88 -0.5 1,507,617 + -1.0 1,507,618 - -0.7 1,507,618 - -0.7 1,507,649 - PP_1321 0.11 +0.3 1,507,666 + PP_1321 0.14 -1.0 1,507,667 - PP_1321 0.15 +0.7 1,507,667 - PP_1321 0.15 +0.9 1,507,701 + PP_1321 0.22 +0.2 1,507,702 - PP_1321 0.22 -1.2 1,507,702 - PP_1321 0.22 -1.1 1,507,702 - PP_1321 0.22 +0.1 1,507,702 - PP_1321 0.22 +0.0 1,507,702 - PP_1321 0.22 -1.0 1,507,776 + PP_1321 0.38 +0.5 1,507,776 + PP_1321 0.38 +0.6 1,507,776 + PP_1321 0.38 -0.6 1,507,777 - PP_1321 0.38 +0.2 1,507,777 - PP_1321 0.38 +0.8 1,507,894 + PP_1321 0.64 -0.9 1,507,894 + PP_1321 0.64 +0.5 1,507,894 + PP_1321 0.64 -2.0 1,507,895 - PP_1321 0.64 +0.0 1,507,895 - PP_1321 0.64 -0.6 1,507,895 - PP_1321 0.64 -0.3 1,507,895 - PP_1321 0.64 +0.6 1,507,895 - PP_1321 0.64 -0.7 1,507,918 - PP_1321 0.69 -1.2 1,508,059 + +2.6 1,508,060 - -0.9 1,508,133 + -0.3 1,508,475 + PP_1322 0.61 -2.5 1,508,476 - PP_1322 0.61 +1.6 1,508,692 + +0.7
Or see this region's nucleotide sequence