Strain Fitness in Pseudomonas putida KT2440 around PP_1116

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
1,275,703	+				+0.4
1,275,703	+				-1.8
1,275,813	+				+0.6
1,275,814	-				-1.3
1,275,814	-				-1.2
1,275,814	-				+0.5
1,275,903	-				-1.0
1,275,927	-				+1.0
1,275,947	+				+0.7
1,275,966	+				+1.5
1,275,994	+				-0.3
1,275,994	+				-0.4
1,275,994	+				-1.3
1,275,994	+				-0.7
1,275,995	-				-0.1
1,276,079	+		PP_5464	0.14	+0.6
1,276,079	+		PP_5464	0.14	-0.8
1,276,079	+		PP_5464	0.14	-0.5
1,276,080	-		PP_5464	0.14	-1.3
1,276,080	-				+1.3
1,276,099	+		PP_5464	0.19	+0.7
1,276,099	+		PP_5464	0.19	+1.5
1,276,099	+		PP_5464	0.19	-0.8
1,276,100	-		PP_5464	0.19	-0.8
1,276,100	-		PP_5464	0.19	+1.1
1,276,100	-		PP_5464	0.19	+2.5
1,276,148	+		PP_5464	0.30	-1.8
1,276,148	+		PP_5464	0.30	+0.9
1,276,148	+		PP_5464	0.30	-1.8
1,276,155	+		PP_5464	0.32	+0.7
1,276,197	+		PP_5464	0.41	-1.0
1,276,197	+		PP_5464	0.41	+1.0
1,276,197	+		PP_5464	0.41	-0.7
1,276,198	-		PP_5464	0.41	-0.7
1,276,198	-		PP_5464	0.41	-1.4
1,276,198	-		PP_5464	0.41	+0.4
1,276,198	-		PP_5464	0.41	+0.1
1,276,198	-		PP_5464	0.41	-0.5
1,276,198	-		PP_5464	0.41	-0.0
1,276,212	+		PP_5464	0.45	-0.3
1,276,213	-		PP_5464	0.45	-2.2
1,276,270	-		PP_5464	0.58	-0.2
1,276,308	+		PP_5464	0.66	-0.9
1,276,308	+		PP_5464	0.66	+2.0
1,276,309	-		PP_5464	0.67	+0.1
1,276,345	-		PP_5464	0.75	+1.5
1,276,366	+		PP_5464	0.80	-2.0
1,276,377	+		PP_5464	0.82	-0.5
1,276,377	+		PP_5464	0.82	+0.4
1,276,378	-		PP_5464	0.82	-1.2
1,276,408	+		PP_5464	0.89	-0.7
1,276,409	-		PP_5464	0.89	-0.1
1,276,428	+				+1.0
1,276,429	-				-0.2
1,276,518	-				-0.6
1,276,518	-				+0.3
1,276,544	-				+1.0
1,276,585	+				-0.9
1,276,586	-				+0.2
1,276,586	-				+0.3
1,276,598	-				-0.9
1,276,638	+				-2.1
1,276,639	-				-1.9
1,276,648	-				-0.8
1,276,651	-				+0.0
1,276,651	-				-0.9
1,276,660	+				+1.1
1,276,660	+				-0.7
1,276,660	+				+0.8
1,276,660	+				-0.4
1,276,661	-				-0.8
1,276,661	-				-0.3
1,276,661	-				+0.0
1,276,695	+				-1.2
1,276,695	+				-1.4
1,276,730	+		PP_1116	0.10	-0.7
1,276,761	+		PP_1116	0.14	-1.8
1,276,762	-		PP_1116	0.14	+0.2
1,276,829	-		PP_1116	0.24	-1.4
1,276,847	+		PP_1116	0.26	-1.4
1,276,847	+		PP_1116	0.26	-1.5
1,276,847	+		PP_1116	0.26	-1.4
1,276,869	-		PP_1116	0.30	-1.6
1,276,869	-		PP_1116	0.30	-0.4
1,276,869	-		PP_1116	0.30	+0.6
1,276,933	-		PP_1116	0.39	-0.6
1,277,094	+		PP_1116	0.61	+0.3
1,277,095	-		PP_1116	0.61	-0.5
1,277,288	-		PP_1116	0.88	-1.1
1,277,288	-		PP_1116	0.88	-1.9
1,277,310	+				-1.4
1,277,310	+				+0.4
1,277,336	+				+1.5
1,277,340	+				-1.7
1,277,341	-				-0.5
1,277,350	+				-1.9
1,277,351	-				+0.1
1,277,389	-				-1.6
1,277,444	-				+0.2
1,277,646	+				+0.4
1,277,677	-				-1.9
1,277,677	-				-1.1
1,277,677	-				+0.9
1,277,677	-				-0.2
1,277,677	-				+1.5
1,277,719	+				-0.1
1,277,753	-				-1.0
1,277,769	+				+0.6
1,277,797	+				+1.3
1,277,798	-				+0.9
1,277,814	+				+0.5
1,277,897	-				+0.3
1,277,930	-				-0.1
1,277,965	+				+2.9
1,277,967	+				+1.0
1,277,967	+				+1.9
1,277,967	+				+0.7
1,277,967	+				-0.2
1,277,968	-				-0.3
1,277,968	-				+0.4
1,277,968	-				-2.0
1,278,039	+		PP_5465	0.36	+1.8
1,278,039	+		PP_5465	0.36	-1.4
1,278,039	+		PP_5465	0.36	-0.1
1,278,040	-		PP_5465	0.36	-0.9
1,278,057	-		PP_5465	0.44	+1.4
1,278,087	+		PP_5465	0.59	+0.7
1,278,097	-		PP_5465	0.63	-0.9
1,278,126	+		PP_5465	0.77	-2.1
1,278,127	-		PP_5465	0.78	-2.0
1,278,127	-		PP_5465	0.78	-1.8
1,278,132	+		PP_5465	0.80	-1.9
1,278,132	+		PP_5465	0.80	+0.9
1,278,133	-		PP_5465	0.80	-1.3
1,278,138	+		PP_5465	0.83	-0.1
1,278,138	+		PP_5465	0.83	-0.4
1,278,138	+		PP_5465	0.83	-0.4
1,278,139	-		PP_5465	0.83	+1.4
1,278,139	-		PP_5465	0.83	-1.4
1,278,139	-		PP_5465	0.83	-0.3
1,278,139	-		PP_5465	0.83	+0.8
1,278,139	-		PP_5465	0.83	-0.5
1,278,311	+				-0.5
1,278,311	+				-0.1
1,278,345	+				-2.4
1,278,352	+				+0.1
1,278,353	-				-0.0