Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1098 and PP_1099 are separated by 167 nucleotides PP_1099 and PP_1100 are separated by 327 nucleotides PP_1100 and PP_1101 are separated by 61 nucleotides
PP_1098: PP_1098 - ATP-dependent Fe-S cluster transferase, at 1,255,659 to 1,256,753
_1098
PP_1099: PP_1099 - Cold shock protein CapB, at 1,256,921 to 1,257,130
_1099
PP_1100: PP_1100 - Deoxycytidine triphosphate deaminase, at 1,257,458 to 1,258,024
_1100
PP_1101: PP_1101 - conserved protein of unknown function, at 1,258,086 to 1,258,346
_1101
Position (kb)
1256
1257
1258 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1256.795 kb on - strand at 1256.853 kb on - strand at 1256.904 kb on + strand at 1256.904 kb on + strand at 1256.912 kb on + strand at 1256.913 kb on - strand at 1256.981 kb on - strand, within PP_1099 at 1257.103 kb on + strand, within PP_1099 at 1257.104 kb on - strand, within PP_1099 at 1257.104 kb on - strand, within PP_1099 at 1257.104 kb on - strand, within PP_1099 at 1257.243 kb on - strand at 1257.388 kb on - strand at 1257.411 kb on + strand at 1257.411 kb on + strand at 1257.584 kb on + strand at 1257.591 kb on - strand, within PP_1100 at 1257.591 kb on - strand at 1257.747 kb on - strand, within PP_1100 at 1257.797 kb on + strand at 1257.966 kb on - strand at 1257.987 kb on - strand at 1258.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,256,795 - -2.3 1,256,853 - +0.6 1,256,904 + +0.4 1,256,904 + +1.6 1,256,912 + +4.0 1,256,913 - -1.7 1,256,981 - PP_1099 0.29 -1.1 1,257,103 + PP_1099 0.87 -0.7 1,257,104 - PP_1099 0.87 -1.0 1,257,104 - PP_1099 0.87 +0.0 1,257,104 - PP_1099 0.87 +2.7 1,257,243 - +1.1 1,257,388 - +1.3 1,257,411 + +0.1 1,257,411 + +0.0 1,257,584 + +1.3 1,257,591 - PP_1100 0.23 +0.7 1,257,591 - +1.0 1,257,747 - PP_1100 0.51 +0.5 1,257,797 + +0.1 1,257,966 - +0.8 1,257,987 - +1.6 1,258,072 + -0.1
Or see this region's nucleotide sequence