Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1039 and PP_1040 overlap by 4 nucleotides PP_1040 and PP_1041 overlap by 1 nucleotides PP_1041 and PP_1042 are separated by 145 nucleotides PP_1042 and PP_1043 are separated by 129 nucleotides
PP_1039: PP_1039 - sec-independent protein translocase protein, at 1,189,052 to 1,189,813
_1039
PP_1040: PP_1040 - Sec-independent protein translocase TatB, at 1,189,810 to 1,190,100
_1040
PP_1041: PP_1041 - twin arginine protein export complex subunit, at 1,190,100 to 1,190,333
_1041
PP_1042: PP_1042 - Type II secretion system protein K, at 1,190,479 to 1,191,444
_1042
PP_1043: PP_1043 - putative phosphatase, at 1,191,574 to 1,193,649
_1043
Position (kb)
1190
1191
1192 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1189.667 kb on + strand, within PP_1039 at 1189.677 kb on + strand, within PP_1039 at 1189.677 kb on + strand, within PP_1039 at 1189.677 kb on + strand, within PP_1039 at 1189.678 kb on - strand, within PP_1039 at 1189.678 kb on - strand, within PP_1039 at 1189.678 kb on - strand, within PP_1039 at 1189.678 kb on - strand, within PP_1039 at 1190.271 kb on - strand, within PP_1041 at 1190.299 kb on - strand, within PP_1041 at 1190.349 kb on - strand at 1190.349 kb on - strand at 1190.349 kb on - strand at 1190.384 kb on + strand at 1190.421 kb on - strand at 1190.731 kb on + strand, within PP_1042 at 1190.842 kb on + strand, within PP_1042 at 1190.843 kb on - strand, within PP_1042 at 1190.847 kb on - strand, within PP_1042 at 1191.142 kb on + strand, within PP_1042 at 1191.142 kb on + strand, within PP_1042 at 1191.143 kb on - strand, within PP_1042 at 1191.143 kb on - strand, within PP_1042 at 1191.143 kb on - strand, within PP_1042 at 1191.396 kb on - strand at 1191.443 kb on - strand at 1191.460 kb on + strand at 1191.461 kb on - strand at 1191.679 kb on - strand at 1191.679 kb on - strand at 1191.680 kb on + strand at 1191.681 kb on - strand at 1191.742 kb on - strand at 1191.882 kb on - strand, within PP_1043 at 1192.169 kb on + strand, within PP_1043 at 1192.169 kb on + strand, within PP_1043 at 1192.171 kb on + strand, within PP_1043 at 1192.171 kb on + strand, within PP_1043 at 1192.171 kb on + strand, within PP_1043 at 1192.171 kb on + strand, within PP_1043 at 1192.171 kb on + strand, within PP_1043 at 1192.172 kb on - strand, within PP_1043 at 1192.172 kb on - strand, within PP_1043 at 1192.172 kb on - strand, within PP_1043 at 1192.283 kb on + strand, within PP_1043 at 1192.283 kb on + strand, within PP_1043 at 1192.284 kb on - strand, within PP_1043 at 1192.284 kb on - strand, within PP_1043 at 1192.342 kb on + strand, within PP_1043 at 1192.343 kb on - strand, within PP_1043 at 1192.343 kb on - strand, within PP_1043 at 1192.377 kb on - strand, within PP_1043 at 1192.377 kb on - strand, within PP_1043
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,189,667 + PP_1039 0.81 -0.8 1,189,677 + PP_1039 0.82 +1.2 1,189,677 + PP_1039 0.82 +2.8 1,189,677 + PP_1039 0.82 -2.5 1,189,678 - PP_1039 0.82 +1.3 1,189,678 - PP_1039 0.82 +0.5 1,189,678 - PP_1039 0.82 +1.1 1,189,678 - PP_1039 0.82 -0.8 1,190,271 - PP_1041 0.73 -1.5 1,190,299 - PP_1041 0.85 +0.2 1,190,349 - +0.4 1,190,349 - -0.2 1,190,349 - -1.7 1,190,384 + +2.2 1,190,421 - +1.5 1,190,731 + PP_1042 0.26 -0.8 1,190,842 + PP_1042 0.38 +2.3 1,190,843 - PP_1042 0.38 -1.9 1,190,847 - PP_1042 0.38 -0.8 1,191,142 + PP_1042 0.69 +1.6 1,191,142 + PP_1042 0.69 +1.5 1,191,143 - PP_1042 0.69 +1.3 1,191,143 - PP_1042 0.69 -0.2 1,191,143 - PP_1042 0.69 +0.2 1,191,396 - -2.1 1,191,443 - -1.9 1,191,460 + +0.2 1,191,461 - +1.5 1,191,679 - -0.9 1,191,679 - -0.3 1,191,680 + +1.3 1,191,681 - -1.2 1,191,742 - -1.1 1,191,882 - PP_1043 0.15 +0.2 1,192,169 + PP_1043 0.29 -0.0 1,192,169 + PP_1043 0.29 +1.3 1,192,171 + PP_1043 0.29 +0.7 1,192,171 + PP_1043 0.29 -1.5 1,192,171 + PP_1043 0.29 +0.3 1,192,171 + PP_1043 0.29 -0.3 1,192,171 + PP_1043 0.29 -1.5 1,192,172 - PP_1043 0.29 -1.3 1,192,172 - PP_1043 0.29 +0.2 1,192,172 - PP_1043 0.29 +0.8 1,192,283 + PP_1043 0.34 -0.6 1,192,283 + PP_1043 0.34 -0.0 1,192,284 - PP_1043 0.34 +0.8 1,192,284 - PP_1043 0.34 +2.5 1,192,342 + PP_1043 0.37 -0.4 1,192,343 - PP_1043 0.37 +0.8 1,192,343 - PP_1043 0.37 +0.4 1,192,377 - PP_1043 0.39 -0.3 1,192,377 - PP_1043 0.39 -0.7
Or see this region's nucleotide sequence