Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1021 and PP_1022 are separated by 211 nucleotides PP_1022 and PP_1023 overlap by 29 nucleotides PP_1023 and PP_1024 overlap by 10 nucleotides
PP_1021: PP_1021 - DNA-binding transcriptional regulator, at 1,164,541 to 1,165,404
_1021
PP_1022: PP_1022 - glucose 6-phosphate-1-dehydrogenase, at 1,165,616 to 1,167,085
_1022
PP_1023: PP_1023 - 6-phosphogluconolactonase, at 1,167,057 to 1,167,785
_1023
PP_1024: PP_1024 - KHG/KDPG aldolase, at 1,167,776 to 1,168,483
_1024
Position (kb)
1165
1166
1167
1168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1164.655 kb on + strand, within PP_1021 at 1164.656 kb on - strand, within PP_1021 at 1164.656 kb on - strand, within PP_1021 at 1164.701 kb on + strand, within PP_1021 at 1164.701 kb on + strand, within PP_1021 at 1164.702 kb on - strand, within PP_1021 at 1164.702 kb on - strand, within PP_1021 at 1164.702 kb on - strand, within PP_1021 at 1165.148 kb on - strand, within PP_1021 at 1165.163 kb on + strand, within PP_1021 at 1165.163 kb on + strand, within PP_1021 at 1165.479 kb on - strand at 1165.503 kb on + strand at 1165.529 kb on + strand at 1165.552 kb on + strand at 1165.834 kb on + strand, within PP_1022 at 1165.834 kb on + strand, within PP_1022 at 1165.877 kb on + strand, within PP_1022 at 1165.910 kb on + strand, within PP_1022 at 1165.958 kb on + strand, within PP_1022 at 1165.958 kb on + strand, within PP_1022 at 1166.141 kb on + strand, within PP_1022 at 1166.242 kb on + strand, within PP_1022 at 1166.471 kb on + strand, within PP_1022 at 1166.471 kb on + strand, within PP_1022 at 1166.582 kb on - strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.681 kb on + strand, within PP_1022 at 1166.716 kb on + strand, within PP_1022 at 1166.870 kb on + strand, within PP_1022 at 1166.870 kb on + strand, within PP_1022 at 1166.871 kb on - strand, within PP_1022 at 1166.981 kb on - strand at 1166.981 kb on - strand at 1166.981 kb on - strand at 1167.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,164,655 + PP_1021 0.13 -0.1 1,164,656 - PP_1021 0.13 +0.6 1,164,656 - PP_1021 0.13 -0.1 1,164,701 + PP_1021 0.19 +0.4 1,164,701 + PP_1021 0.19 +0.3 1,164,702 - PP_1021 0.19 -0.9 1,164,702 - PP_1021 0.19 +0.4 1,164,702 - PP_1021 0.19 -0.5 1,165,148 - PP_1021 0.70 -0.6 1,165,163 + PP_1021 0.72 -0.4 1,165,163 + PP_1021 0.72 +0.3 1,165,479 - -1.6 1,165,503 + -0.6 1,165,529 + -0.3 1,165,552 + -1.7 1,165,834 + PP_1022 0.15 +0.9 1,165,834 + PP_1022 0.15 -1.6 1,165,877 + PP_1022 0.18 -0.5 1,165,910 + PP_1022 0.20 +0.9 1,165,958 + PP_1022 0.23 +0.3 1,165,958 + PP_1022 0.23 +0.6 1,166,141 + PP_1022 0.36 -1.8 1,166,242 + PP_1022 0.43 +0.1 1,166,471 + PP_1022 0.58 -0.8 1,166,471 + PP_1022 0.58 -1.2 1,166,582 - PP_1022 0.66 +1.7 1,166,681 + PP_1022 0.72 -0.2 1,166,681 + PP_1022 0.72 +1.3 1,166,681 + PP_1022 0.72 -3.1 1,166,681 + PP_1022 0.72 -1.8 1,166,681 + PP_1022 0.72 +1.1 1,166,716 + PP_1022 0.75 -0.2 1,166,870 + PP_1022 0.85 -2.3 1,166,870 + PP_1022 0.85 -0.7 1,166,871 - PP_1022 0.85 -0.4 1,166,981 - +1.0 1,166,981 - +2.1 1,166,981 - +0.8 1,167,079 + +1.1
Or see this region's nucleotide sequence