Strain Fitness in Pseudomonas putida KT2440 around PP_1002

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.002 mg/ml
1,140,015	-		PP_1001	0.24	-1.5
1,140,027	+		PP_1001	0.25	-0.3
1,140,251	-		PP_1001	0.43	-1.9
1,140,381	+		PP_1001	0.53	-1.4
1,140,426	+		PP_1001	0.57	-0.9
1,140,427	-		PP_1001	0.57	+0.9
1,140,537	+		PP_1001	0.66	-0.8
1,140,537	+		PP_1001	0.66	+1.5
1,140,644	-		PP_1001	0.74	+1.1
1,140,644	-		PP_1001	0.74	-1.4
1,140,945	+				-0.8
1,140,946	-				-2.0
1,140,946	-				+0.4
1,141,023	+				+0.2
1,141,032	+				-0.6
1,141,033	-				-2.3
1,141,033	-				-0.0
1,141,174	-		PP_1002	0.12	-1.1
1,141,192	-		PP_1002	0.13	-1.1
1,141,192	-		PP_1002	0.13	-1.4
1,141,210	-		PP_1002	0.15	-1.7
1,141,210	-		PP_1002	0.15	+0.1
1,141,269	+		PP_1002	0.19	+0.1
1,141,270	-		PP_1002	0.19	-0.3
1,141,303	-		PP_1002	0.21	-1.6
1,141,320	+		PP_1002	0.22	+0.5
1,141,351	-		PP_1002	0.25	-0.7
1,141,365	+		PP_1002	0.25	+1.6
1,141,365	+		PP_1002	0.25	+0.6
1,141,366	-		PP_1002	0.26	+1.1
1,141,366	-		PP_1002	0.26	-2.2
1,141,366	-		PP_1002	0.26	-1.1
1,141,877	-		PP_1002	0.61	-0.1
1,142,042	+		PP_1002	0.73	-1.5
1,142,086	-		PP_1002	0.76	+0.0
1,142,086	-		PP_1002	0.76	-1.4
1,142,176	-		PP_1002	0.82	-1.1
1,142,176	-		PP_1002	0.82	+0.7
1,142,176	-		PP_1002	0.82	-1.1
1,142,176	-		PP_1002	0.82	-1.0
1,142,200	-		PP_1002	0.84	-1.0
1,142,200	-		PP_1002	0.84	-1.7
1,142,200	-		PP_1002	0.84	-1.2
1,142,205	+		PP_1002	0.84	-0.5
1,142,205	+		PP_1002	0.84	-1.4
1,142,205	+		PP_1002	0.84	-1.3
1,142,205	+		PP_1002	0.84	-1.0
1,142,206	-		PP_1002	0.84	-1.3
1,142,354	-				-1.3
1,142,391	-				-0.9
1,142,430	+				-2.1
1,142,430	+				-0.6
1,142,431	-				+1.0
1,142,466	-				+0.6
1,142,466	-				+1.8
1,142,495	+				-0.3
1,142,495	+				+0.1
1,142,583	+				-0.9
1,142,584	-				+0.2
1,142,606	-				+0.9
1,142,644	-				+0.6
1,142,644	-				+0.9
1,142,746	+				-0.8
1,142,771	-				-1.8
1,142,789	+				+0.1
1,142,790	-				+1.6
1,142,794	+				+2.2
1,142,795	-				+0.3
1,142,810	+				-0.6
1,142,810	+				-0.7
1,142,810	+				-1.1
1,142,810	+				-2.0
1,143,085	+		PP_1003	0.15	+0.5
1,143,086	-		PP_1003	0.15	+2.3
1,143,087	+		PP_1003	0.16	+0.2
1,143,087	+		PP_1003	0.16	-2.0
1,143,087	+		PP_1003	0.16	-1.6
1,143,087	+		PP_1003	0.16	+0.3
1,143,087	+		PP_1003	0.16	-1.4
1,143,088	-		PP_1003	0.16	+1.3
1,143,088	-		PP_1003	0.16	+0.5
1,143,088	-		PP_1003	0.16	-1.4
1,143,088	-		PP_1003	0.16	-1.4
1,143,088	-		PP_1003	0.16	-0.3
1,143,093	+		PP_1003	0.16	-0.0
1,143,094	-		PP_1003	0.16	-0.2
1,143,094	-		PP_1003	0.16	-1.1
1,143,094	-		PP_1003	0.16	-0.0
1,143,117	+		PP_1003	0.18	-0.3
1,143,117	+		PP_1003	0.18	+0.6
1,143,117	+		PP_1003	0.18	+1.2
1,143,118	-		PP_1003	0.18	-1.8
1,143,211	-		PP_1003	0.24	-0.8
1,143,211	-		PP_1003	0.24	+0.5
1,143,211	-		PP_1003	0.24	+0.7
1,143,281	-		PP_1003	0.29	+1.0