Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0997 and PP_0998 are separated by 140 nucleotides PP_0998 and PP_0999 overlap by 4 nucleotides
PP_0997: PP_0997 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 1,135,466 to 1,136,974
_0997
PP_0998: PP_0998 - conserved protein of unknown function, at 1,137,115 to 1,137,543
_0998
PP_0999: PP_0999 - carbamate kinase, at 1,137,540 to 1,138,469
_0999
Position (kb)
1137
1138 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1136.352 kb on - strand, within PP_0997 at 1136.466 kb on + strand, within PP_0997 at 1136.467 kb on - strand, within PP_0997 at 1136.522 kb on - strand, within PP_0997 at 1136.686 kb on + strand, within PP_0997 at 1136.686 kb on + strand at 1136.686 kb on + strand, within PP_0997 at 1136.687 kb on - strand, within PP_0997 at 1136.687 kb on - strand, within PP_0997 at 1136.687 kb on - strand, within PP_0997 at 1136.690 kb on + strand, within PP_0997 at 1136.690 kb on + strand, within PP_0997 at 1136.691 kb on - strand, within PP_0997 at 1136.794 kb on + strand, within PP_0997 at 1136.828 kb on + strand at 1136.859 kb on + strand at 1136.860 kb on - strand at 1136.860 kb on - strand at 1137.009 kb on - strand at 1137.089 kb on - strand at 1137.186 kb on + strand, within PP_0998 at 1137.186 kb on + strand, within PP_0998 at 1137.186 kb on + strand, within PP_0998 at 1137.186 kb on + strand, within PP_0998 at 1137.186 kb on + strand, within PP_0998 at 1137.186 kb on + strand, within PP_0998 at 1137.186 kb on + strand, within PP_0998 at 1137.187 kb on - strand, within PP_0998 at 1137.187 kb on - strand, within PP_0998 at 1137.195 kb on - strand, within PP_0998 at 1137.245 kb on + strand, within PP_0998 at 1137.245 kb on + strand, within PP_0998 at 1137.246 kb on - strand, within PP_0998 at 1137.269 kb on - strand, within PP_0998 at 1137.363 kb on + strand, within PP_0998 at 1137.364 kb on - strand, within PP_0998 at 1137.364 kb on - strand, within PP_0998 at 1137.399 kb on - strand, within PP_0998 at 1137.416 kb on + strand, within PP_0998 at 1137.416 kb on + strand, within PP_0998 at 1137.416 kb on + strand, within PP_0998 at 1137.417 kb on - strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.457 kb on + strand, within PP_0998 at 1137.458 kb on - strand, within PP_0998 at 1137.458 kb on - strand, within PP_0998 at 1137.458 kb on - strand, within PP_0998 at 1137.473 kb on + strand, within PP_0998 at 1137.487 kb on + strand, within PP_0998 at 1137.488 kb on - strand, within PP_0998 at 1137.547 kb on + strand at 1137.547 kb on + strand at 1137.775 kb on + strand, within PP_0999 at 1137.775 kb on + strand, within PP_0999 at 1137.776 kb on - strand, within PP_0999 at 1137.898 kb on + strand, within PP_0999 at 1137.898 kb on + strand, within PP_0999 at 1137.898 kb on + strand, within PP_0999 at 1137.898 kb on + strand, within PP_0999 at 1137.899 kb on - strand, within PP_0999 at 1138.018 kb on + strand, within PP_0999 at 1138.087 kb on + strand, within PP_0999 at 1138.279 kb on + strand, within PP_0999 at 1138.388 kb on + strand at 1138.463 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,136,352 - PP_0997 0.59 -0.3 1,136,466 + PP_0997 0.66 +0.2 1,136,467 - PP_0997 0.66 -0.0 1,136,522 - PP_0997 0.70 +0.3 1,136,686 + PP_0997 0.81 -0.0 1,136,686 + +1.1 1,136,686 + PP_0997 0.81 -1.5 1,136,687 - PP_0997 0.81 +0.1 1,136,687 - PP_0997 0.81 -0.2 1,136,687 - PP_0997 0.81 +0.8 1,136,690 + PP_0997 0.81 +0.1 1,136,690 + PP_0997 0.81 -1.8 1,136,691 - PP_0997 0.81 +0.0 1,136,794 + PP_0997 0.88 -1.0 1,136,828 + -0.1 1,136,859 + +0.2 1,136,860 - +0.4 1,136,860 - -0.1 1,137,009 - -0.4 1,137,089 - +0.7 1,137,186 + PP_0998 0.17 +0.8 1,137,186 + PP_0998 0.17 -0.7 1,137,186 + PP_0998 0.17 -0.4 1,137,186 + PP_0998 0.17 -0.6 1,137,186 + PP_0998 0.17 +1.6 1,137,186 + PP_0998 0.17 +2.2 1,137,186 + PP_0998 0.17 -1.4 1,137,187 - PP_0998 0.17 +2.5 1,137,187 - PP_0998 0.17 +0.3 1,137,195 - PP_0998 0.19 -1.0 1,137,245 + PP_0998 0.30 -1.1 1,137,245 + PP_0998 0.30 -1.7 1,137,246 - PP_0998 0.31 -1.9 1,137,269 - PP_0998 0.36 -0.8 1,137,363 + PP_0998 0.58 -1.8 1,137,364 - PP_0998 0.58 -1.0 1,137,364 - PP_0998 0.58 +0.0 1,137,399 - PP_0998 0.66 +0.3 1,137,416 + PP_0998 0.70 -1.3 1,137,416 + PP_0998 0.70 +1.7 1,137,416 + PP_0998 0.70 -1.8 1,137,417 - PP_0998 0.70 -1.1 1,137,457 + PP_0998 0.80 -0.3 1,137,457 + PP_0998 0.80 +1.3 1,137,457 + PP_0998 0.80 -0.7 1,137,457 + PP_0998 0.80 -0.7 1,137,457 + PP_0998 0.80 -1.0 1,137,457 + PP_0998 0.80 +1.3 1,137,457 + PP_0998 0.80 -0.5 1,137,457 + PP_0998 0.80 -0.0 1,137,457 + PP_0998 0.80 -0.1 1,137,458 - PP_0998 0.80 -0.4 1,137,458 - PP_0998 0.80 -1.9 1,137,458 - PP_0998 0.80 +0.7 1,137,473 + PP_0998 0.83 -1.3 1,137,487 + PP_0998 0.87 -0.8 1,137,488 - PP_0998 0.87 -1.3 1,137,547 + +2.2 1,137,547 + -1.0 1,137,775 + PP_0999 0.25 +0.0 1,137,775 + PP_0999 0.25 -1.7 1,137,776 - PP_0999 0.25 -1.2 1,137,898 + PP_0999 0.38 -2.0 1,137,898 + PP_0999 0.38 -1.4 1,137,898 + PP_0999 0.38 +0.0 1,137,898 + PP_0999 0.38 -1.5 1,137,899 - PP_0999 0.39 -0.5 1,138,018 + PP_0999 0.51 -1.7 1,138,087 + PP_0999 0.59 -2.1 1,138,279 + PP_0999 0.79 -1.2 1,138,388 + +0.4 1,138,463 + -0.5
Or see this region's nucleotide sequence