Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0973 and PP_0975 are separated by 369 nucleotides PP_0975 and PP_0976 are separated by 47 nucleotides PP_0976 and PP_0977 are separated by 178 nucleotides
PP_0973: PP_0973 - Nucleoid-associated protein PP_0973, at 1,112,147 to 1,113,154
_0973
PP_0975: PP_0975 - putative DNA-binding protein HU, form N, at 1,113,524 to 1,113,805
_0975
PP_0976: PP_0976 - Ribosomal RNA large subunit methyltransferase F, at 1,113,853 to 1,114,806
_0976
PP_0977: PP_0977 - valine--tRNA ligase, at 1,114,985 to 1,117,831
_0977
Position (kb)
1113
1114
1115 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1112.937 kb on + strand, within PP_0973 at 1112.938 kb on - strand, within PP_0973 at 1112.938 kb on - strand, within PP_0973 at 1112.938 kb on - strand, within PP_0973 at 1113.018 kb on + strand, within PP_0973 at 1113.018 kb on + strand, within PP_0973 at 1113.018 kb on + strand, within PP_0973 at 1113.019 kb on - strand, within PP_0973 at 1113.219 kb on + strand at 1113.220 kb on - strand at 1113.220 kb on - strand at 1113.232 kb on - strand at 1113.232 kb on - strand at 1113.299 kb on + strand at 1113.299 kb on + strand at 1113.300 kb on - strand at 1113.319 kb on + strand at 1113.319 kb on + strand at 1113.319 kb on + strand at 1113.420 kb on + strand at 1113.421 kb on - strand at 1113.421 kb on - strand at 1113.424 kb on + strand at 1113.424 kb on + strand at 1113.424 kb on + strand at 1113.425 kb on - strand at 1113.425 kb on - strand at 1113.425 kb on - strand at 1113.428 kb on + strand at 1113.428 kb on + strand at 1113.428 kb on + strand at 1113.428 kb on + strand at 1113.429 kb on - strand at 1113.429 kb on - strand at 1113.479 kb on - strand at 1113.491 kb on - strand at 1113.804 kb on - strand at 1113.804 kb on - strand at 1113.867 kb on + strand at 1113.868 kb on - strand at 1113.868 kb on - strand at 1113.965 kb on - strand, within PP_0976 at 1113.965 kb on - strand, within PP_0976 at 1114.048 kb on + strand, within PP_0976 at 1114.049 kb on - strand, within PP_0976 at 1114.430 kb on + strand, within PP_0976 at 1114.430 kb on + strand, within PP_0976 at 1114.431 kb on - strand, within PP_0976 at 1114.446 kb on - strand, within PP_0976 at 1114.540 kb on + strand, within PP_0976 at 1114.540 kb on + strand, within PP_0976 at 1114.550 kb on + strand, within PP_0976 at 1114.550 kb on + strand, within PP_0976 at 1114.551 kb on - strand, within PP_0976 at 1114.551 kb on - strand, within PP_0976 at 1114.584 kb on - strand, within PP_0976 at 1114.584 kb on - strand, within PP_0976 at 1114.584 kb on - strand, within PP_0976 at 1114.822 kb on + strand at 1114.822 kb on + strand at 1114.822 kb on + strand at 1114.823 kb on - strand at 1114.824 kb on + strand at 1114.824 kb on + strand at 1114.824 kb on + strand at 1114.956 kb on + strand at 1114.956 kb on + strand at 1114.979 kb on + strand at 1114.979 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 1,112,937 + PP_0973 0.78 +0.5 1,112,938 - PP_0973 0.78 +2.4 1,112,938 - PP_0973 0.78 -1.2 1,112,938 - PP_0973 0.78 -0.8 1,113,018 + PP_0973 0.86 +0.4 1,113,018 + PP_0973 0.86 -0.3 1,113,018 + PP_0973 0.86 -0.0 1,113,019 - PP_0973 0.87 +0.0 1,113,219 + +0.4 1,113,220 - +1.3 1,113,220 - +0.9 1,113,232 - +0.8 1,113,232 - +0.6 1,113,299 + -2.1 1,113,299 + +0.5 1,113,300 - +0.7 1,113,319 + -0.3 1,113,319 + +0.3 1,113,319 + +0.2 1,113,420 + +0.3 1,113,421 - -0.2 1,113,421 - +0.3 1,113,424 + +1.2 1,113,424 + -1.3 1,113,424 + +0.1 1,113,425 - -2.0 1,113,425 - -0.0 1,113,425 - +0.1 1,113,428 + +1.3 1,113,428 + +0.1 1,113,428 + +0.5 1,113,428 + -1.4 1,113,429 - +0.5 1,113,429 - +1.9 1,113,479 - -1.3 1,113,491 - +0.6 1,113,804 - +3.0 1,113,804 - +0.2 1,113,867 + +0.4 1,113,868 - +1.2 1,113,868 - -0.1 1,113,965 - PP_0976 0.12 -1.9 1,113,965 - PP_0976 0.12 -2.1 1,114,048 + PP_0976 0.20 -0.5 1,114,049 - PP_0976 0.21 +0.6 1,114,430 + PP_0976 0.60 -0.9 1,114,430 + PP_0976 0.60 -1.4 1,114,431 - PP_0976 0.61 -0.3 1,114,446 - PP_0976 0.62 -2.0 1,114,540 + PP_0976 0.72 -1.4 1,114,540 + PP_0976 0.72 +1.5 1,114,550 + PP_0976 0.73 +2.0 1,114,550 + PP_0976 0.73 -0.8 1,114,551 - PP_0976 0.73 -2.0 1,114,551 - PP_0976 0.73 +1.5 1,114,584 - PP_0976 0.77 -1.3 1,114,584 - PP_0976 0.77 -1.1 1,114,584 - PP_0976 0.77 -0.3 1,114,822 + -1.8 1,114,822 + +2.0 1,114,822 + +0.4 1,114,823 - +0.5 1,114,824 + +0.8 1,114,824 + -0.2 1,114,824 + -0.1 1,114,956 + -1.7 1,114,956 + -0.2 1,114,979 + -1.1 1,114,979 + +0.7
Or see this region's nucleotide sequence