Strain Fitness in Pseudomonas putida KT2440 around PP_0850

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0847 and PP_0848 are separated by 10 nucleotidesPP_0848 and PP_0849 are separated by 88 nucleotidesPP_0849 and PP_0850 are separated by 28 nucleotidesPP_0850 and PP_0851 are separated by 8 nucleotidesPP_0851 and PP_0852 overlap by 4 nucleotides PP_0847: PP_0847 - ferredoxin, at 982,990 to 983,331 _0847 PP_0848: PP_0848 - donor of Fe2+ and regulator of cysteine desulfurase activity, at 983,342 to 983,542 _0848 PP_0849: PP_0849 - Nucleoside diphosphate kinase, at 983,631 to 984,056 _0849 PP_0850: PP_0850 - Dual-specificity RNA methyltransferase RlmN, at 984,085 to 985,230 _0850 PP_0851: PP_0851 - Type IV pili biogenesis protein PilF, at 985,239 to 986,006 _0851 PP_0852: PP_0852 - Transcriptional regulator, Cro/CI family, at 986,003 to 987,010 _0852 Position (kb) 984 985 986Strain fitness (log2 ratio) -2 -1 0 1 2 3at 983.339 kb on + strandat 983.339 kb on + strandat 983.340 kb on - strandat 983.340 kb on - strandat 983.340 kb on - strandat 983.340 kb on - strandat 983.340 kb on - strandat 983.365 kb on - strand, within PP_0848at 983.417 kb on + strand, within PP_0848at 983.417 kb on + strand, within PP_0848at 983.418 kb on - strand, within PP_0848at 983.418 kb on - strand, within PP_0848at 983.540 kb on + strandat 983.540 kb on + strandat 983.583 kb on + strandat 983.583 kb on + strandat 984.043 kb on + strandat 984.043 kb on + strandat 984.044 kb on - strandat 984.067 kb on - strandat 984.096 kb on + strandat 984.096 kb on + strandat 984.156 kb on + strandat 984.156 kb on + strandat 984.242 kb on + strand, within PP_0850at 984.242 kb on + strand, within PP_0850at 984.242 kb on + strand, within PP_0850at 984.243 kb on - strand, within PP_0850at 984.243 kb on - strand, within PP_0850at 984.333 kb on + strand, within PP_0850at 984.333 kb on + strand, within PP_0850at 984.334 kb on - strand, within PP_0850at 984.379 kb on + strand, within PP_0850at 984.379 kb on + strand, within PP_0850at 984.380 kb on - strand, within PP_0850at 984.380 kb on - strand, within PP_0850at 984.847 kb on + strand, within PP_0850at 984.847 kb on + strand, within PP_0850at 984.848 kb on - strand, within PP_0850at 984.848 kb on - strand, within PP_0850at 984.889 kb on + strand, within PP_0850at 984.889 kb on + strand, within PP_0850at 984.943 kb on + strand, within PP_0850at 984.959 kb on + strand, within PP_0850at 985.086 kb on + strand, within PP_0850at 985.149 kb on + strandat 985.168 kb on + strandat 985.169 kb on - strandat 985.169 kb on - strandat 985.169 kb on - strandat 985.235 kb on + strandat 985.236 kb on - strandat 985.236 kb on - strandat 985.362 kb on + strand, within PP_0851at 985.362 kb on + strand, within PP_0851at 985.362 kb on + strand, within PP_0851at 985.363 kb on - strand, within PP_0851at 985.363 kb on - strand, within PP_0851at 985.363 kb on - strand, within PP_0851at 985.384 kb on - strand, within PP_0851at 985.543 kb on + strand, within PP_0851at 985.543 kb on + strand, within PP_0851at 985.543 kb on + strand, within PP_0851at 985.544 kb on - strand, within PP_0851at 985.576 kb on - strand, within PP_0851at 985.591 kb on - strand, within PP_0851at 985.653 kb on + strand, within PP_0851at 985.731 kb on + strand, within PP_0851at 985.731 kb on + strand, within PP_0851at 985.731 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.815 kb on + strand, within PP_0851at 985.833 kb on + strand, within PP_0851at 985.833 kb on + strand, within PP_0851at 985.833 kb on + strand, within PP_0851at 985.836 kb on + strand, within PP_0851at 985.836 kb on + strand, within PP_0851at 985.836 kb on + strand, within PP_0851at 985.836 kb on + strand, within PP_0851at 985.836 kb on + strand, within PP_0851at 985.948 kb on + strandat 985.959 kb on + strandat 985.986 kb on + strandat 985.986 kb on + strandat 986.025 kb on + strandat 986.025 kb on + strandat 986.110 kb on + strand, within PP_0852at 986.110 kb on + strand, within PP_0852at 986.179 kb on + strand, within PP_0852at 986.195 kb on + strand, within PP_0852

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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983,339 + +0.4
983,339 + +0.5
983,340 - +2.0
983,340 - -1.0
983,340 - +0.7
983,340 - -0.7
983,340 - +0.3
983,365 - PP_0848 0.11 +1.6
983,417 + PP_0848 0.37 -0.9
983,417 + PP_0848 0.37 +1.0
983,418 - PP_0848 0.38 +1.3
983,418 - PP_0848 0.38 -0.4
983,540 + +0.6
983,540 + -2.1
983,583 + +1.0
983,583 + +0.9
984,043 + +1.2
984,043 + -0.1
984,044 - +1.6
984,067 - -0.6
984,096 + +1.1
984,096 + -0.3
984,156 + +1.2
984,156 + -0.7
984,242 + PP_0850 0.14 +1.9
984,242 + PP_0850 0.14 -0.5
984,242 + PP_0850 0.14 +1.8
984,243 - PP_0850 0.14 +0.4
984,243 - PP_0850 0.14 +0.3
984,333 + PP_0850 0.22 +0.4
984,333 + PP_0850 0.22 +0.3
984,334 - PP_0850 0.22 -1.3
984,379 + PP_0850 0.26 +1.8
984,379 + PP_0850 0.26 +0.2
984,380 - PP_0850 0.26 +1.7
984,380 - PP_0850 0.26 -0.0
984,847 + PP_0850 0.66 +0.7
984,847 + PP_0850 0.66 +0.9
984,848 - PP_0850 0.67 +0.0
984,848 - PP_0850 0.67 -1.8
984,889 + PP_0850 0.70 -2.1
984,889 + PP_0850 0.70 +2.0
984,943 + PP_0850 0.75 -0.0
984,959 + PP_0850 0.76 +0.3
985,086 + PP_0850 0.87 -0.1
985,149 + -0.6
985,168 + +0.7
985,169 - -1.1
985,169 - -1.5
985,169 - -0.4
985,235 + -0.5
985,236 - -1.2
985,236 - +0.1
985,362 + PP_0851 0.16 +1.2
985,362 + PP_0851 0.16 +2.3
985,362 + PP_0851 0.16 +1.5
985,363 - PP_0851 0.16 -0.3
985,363 - PP_0851 0.16 -1.0
985,363 - PP_0851 0.16 -1.0
985,384 - PP_0851 0.19 -0.1
985,543 + PP_0851 0.40 -0.2
985,543 + PP_0851 0.40 -0.5
985,543 + PP_0851 0.40 +0.5
985,544 - PP_0851 0.40 +0.9
985,576 - PP_0851 0.44 +2.2
985,591 - PP_0851 0.46 -0.7
985,653 + PP_0851 0.54 -1.9
985,731 + PP_0851 0.64 -0.0
985,731 + PP_0851 0.64 +0.1
985,731 + PP_0851 0.64 -0.8
985,815 + PP_0851 0.75 +2.9
985,815 + PP_0851 0.75 -0.6
985,815 + PP_0851 0.75 -0.5
985,815 + PP_0851 0.75 +0.3
985,815 + PP_0851 0.75 -1.3
985,815 + PP_0851 0.75 -0.8
985,815 + PP_0851 0.75 +1.2
985,815 + PP_0851 0.75 -0.2
985,815 + PP_0851 0.75 +1.2
985,815 + PP_0851 0.75 +0.9
985,833 + PP_0851 0.77 +0.0
985,833 + PP_0851 0.77 -0.2
985,833 + PP_0851 0.77 -2.6
985,836 + PP_0851 0.78 -2.2
985,836 + PP_0851 0.78 -2.0
985,836 + PP_0851 0.78 +0.0
985,836 + PP_0851 0.78 -1.6
985,836 + PP_0851 0.78 -2.1
985,948 + +0.0
985,959 + +0.0
985,986 + -0.8
985,986 + +1.2
986,025 + +0.2
986,025 + -1.8
986,110 + PP_0852 0.11 +0.1
986,110 + PP_0852 0.11 -0.1
986,179 + PP_0852 0.17 -0.5
986,195 + PP_0852 0.19 -0.3

Or see this region's nucleotide sequence