Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0832 and PP_0833 are separated by 14 nucleotides PP_0833 and PP_0834 are separated by 42 nucleotides PP_0834 and PP_0835 are separated by 63 nucleotides
PP_0832: PP_0832 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 969,088 to 970,137
_0832
PP_0833: PP_0833 - queuine tRNA-ribosyltransferase, at 970,152 to 971,267
_0833
PP_0834: PP_0834 - Sec translocation complex subunit YajC, at 971,310 to 971,645
_0834
PP_0835: PP_0835 - Sec Translocation Complex SecD subunit, at 971,709 to 973,571
_0835
Position (kb) 970
971
972 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 969.232 kb on + strand, within PP_0832 at 969.232 kb on + strand, within PP_0832 at 969.233 kb on - strand, within PP_0832 at 969.395 kb on + strand, within PP_0832 at 969.396 kb on - strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.529 kb on + strand, within PP_0832 at 969.531 kb on + strand, within PP_0832 at 969.532 kb on - strand, within PP_0832 at 969.569 kb on - strand, within PP_0832 at 969.753 kb on + strand, within PP_0832 at 970.037 kb on - strand at 970.076 kb on - strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.254 kb on + strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.255 kb on - strand at 970.435 kb on + strand, within PP_0833 at 970.567 kb on + strand, within PP_0833 at 970.716 kb on + strand, within PP_0833 at 970.955 kb on + strand, within PP_0833 at 970.985 kb on + strand, within PP_0833 at 971.060 kb on + strand, within PP_0833 at 971.085 kb on + strand, within PP_0833 at 971.135 kb on + strand, within PP_0833 at 971.201 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.298 kb on + strand at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.418 kb on + strand, within PP_0834 at 971.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 969,232 + PP_0832 0.14 -0.3 969,232 + PP_0832 0.14 +0.9 969,233 - PP_0832 0.14 -0.1 969,395 + PP_0832 0.29 -1.5 969,396 - PP_0832 0.29 +1.0 969,529 + PP_0832 0.42 -1.2 969,529 + PP_0832 0.42 +0.5 969,529 + PP_0832 0.42 +0.2 969,531 + PP_0832 0.42 +0.0 969,532 - PP_0832 0.42 +3.0 969,569 - PP_0832 0.46 +0.6 969,753 + PP_0832 0.63 +1.0 970,037 - +1.8 970,076 - +2.0 970,254 + -0.1 970,254 + +0.0 970,254 + +0.5 970,255 - +0.3 970,255 - +0.5 970,255 - +1.4 970,435 + PP_0833 0.25 +1.8 970,567 + PP_0833 0.37 +1.0 970,716 + PP_0833 0.51 -0.3 970,955 + PP_0833 0.72 +0.7 970,985 + PP_0833 0.75 +1.1 971,060 + PP_0833 0.81 +0.9 971,085 + PP_0833 0.84 +0.1 971,135 + PP_0833 0.88 +0.3 971,201 + +0.5 971,298 + +0.9 971,298 + -1.9 971,298 + -0.4 971,418 + PP_0834 0.32 -0.0 971,418 + PP_0834 0.32 -1.8 971,418 + PP_0834 0.32 -0.1 971,639 + +0.5
Or see this region's nucleotide sequence