Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0826 and PP_0827 overlap by 4 nucleotides PP_0827 and PP_0828 are separated by 36 nucleotides PP_0828 and PP_0829 are separated by 318 nucleotides PP_0829 and PP_0830 are separated by 10 nucleotides
PP_0826: PP_0826 - phosphonate ABC transporter, permease protein, at 964,195 to 965,022
_0826
PP_0827: PP_0827 - putative phosphonate transport system permease protein PtxC, at 965,019 to 965,786
_0827
PP_0828: PP_0828 - conserved membrane protein of unknown function, at 965,823 to 966,716
_0828
PP_0829: PP_0829 - conserved protein of unknown function, at 967,035 to 967,331
_0829
PP_0830: PP_0830 - Acetyltransferase, GNAT family, at 967,342 to 967,821
_0830
Position (kb) 965
966
967 Strain fitness (log2 ratio) -2
-1
0
1
2 at 964.823 kb on - strand, within PP_0826 at 964.831 kb on + strand, within PP_0826 at 965.085 kb on + strand at 965.580 kb on - strand, within PP_0827 at 965.664 kb on + strand, within PP_0827 at 965.664 kb on + strand, within PP_0827 at 965.664 kb on + strand, within PP_0827 at 965.717 kb on + strand at 965.717 kb on + strand at 965.896 kb on - strand at 965.900 kb on + strand at 965.900 kb on + strand at 965.900 kb on + strand at 965.901 kb on - strand at 965.901 kb on - strand at 965.901 kb on - strand at 965.901 kb on - strand at 966.083 kb on - strand, within PP_0828 at 966.083 kb on - strand, within PP_0828 at 966.088 kb on + strand, within PP_0828 at 966.088 kb on + strand, within PP_0828 at 966.088 kb on + strand, within PP_0828 at 966.089 kb on - strand, within PP_0828 at 966.089 kb on - strand, within PP_0828 at 966.089 kb on - strand, within PP_0828 at 966.107 kb on + strand, within PP_0828 at 966.150 kb on + strand, within PP_0828 at 966.151 kb on - strand, within PP_0828 at 966.151 kb on - strand, within PP_0828 at 966.206 kb on - strand, within PP_0828 at 966.358 kb on + strand, within PP_0828 at 966.366 kb on - strand, within PP_0828 at 966.664 kb on + strand at 966.665 kb on - strand at 966.851 kb on + strand at 966.852 kb on - strand at 966.852 kb on - strand at 966.852 kb on - strand at 966.852 kb on - strand at 966.909 kb on - strand at 966.980 kb on + strand at 966.981 kb on - strand at 967.101 kb on + strand, within PP_0829 at 967.101 kb on + strand, within PP_0829 at 967.102 kb on - strand, within PP_0829 at 967.102 kb on - strand, within PP_0829 at 967.106 kb on + strand, within PP_0829 at 967.118 kb on - strand, within PP_0829 at 967.118 kb on - strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.187 kb on + strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.188 kb on - strand, within PP_0829 at 967.224 kb on + strand, within PP_0829 at 967.225 kb on - strand, within PP_0829 at 967.225 kb on - strand, within PP_0829 at 967.225 kb on - strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.267 kb on + strand, within PP_0829 at 967.268 kb on - strand, within PP_0829 at 967.268 kb on - strand, within PP_0829 at 967.268 kb on - strand, within PP_0829 at 967.268 kb on - strand, within PP_0829 at 967.268 kb on - strand, within PP_0829 at 967.343 kb on + strand at 967.351 kb on + strand at 967.351 kb on + strand at 967.352 kb on - strand at 967.352 kb on - strand at 967.499 kb on + strand, within PP_0830 at 967.499 kb on + strand, within PP_0830 at 967.500 kb on - strand, within PP_0830 at 967.500 kb on - strand, within PP_0830 at 967.500 kb on - strand, within PP_0830 at 967.547 kb on - strand, within PP_0830 at 967.547 kb on - strand, within PP_0830 at 967.579 kb on + strand, within PP_0830 at 967.597 kb on + strand, within PP_0830 at 967.597 kb on + strand, within PP_0830 at 967.598 kb on - strand, within PP_0830 at 967.598 kb on - strand, within PP_0830 at 967.598 kb on - strand, within PP_0830
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 964,823 - PP_0826 0.76 +0.6 964,831 + PP_0826 0.77 -1.0 965,085 + -0.6 965,580 - PP_0827 0.73 +1.1 965,664 + PP_0827 0.84 +0.5 965,664 + PP_0827 0.84 -1.0 965,664 + PP_0827 0.84 +0.1 965,717 + -0.6 965,717 + -0.8 965,896 - +1.7 965,900 + +0.1 965,900 + -0.5 965,900 + -0.3 965,901 - -0.8 965,901 - +0.6 965,901 - +1.8 965,901 - -0.3 966,083 - PP_0828 0.29 +0.5 966,083 - PP_0828 0.29 +1.0 966,088 + PP_0828 0.30 -0.3 966,088 + PP_0828 0.30 -0.3 966,088 + PP_0828 0.30 -1.4 966,089 - PP_0828 0.30 -1.1 966,089 - PP_0828 0.30 -2.1 966,089 - PP_0828 0.30 -1.5 966,107 + PP_0828 0.32 -0.3 966,150 + PP_0828 0.37 -1.3 966,151 - PP_0828 0.37 -1.7 966,151 - PP_0828 0.37 -1.1 966,206 - PP_0828 0.43 -0.3 966,358 + PP_0828 0.60 +1.2 966,366 - PP_0828 0.61 -1.9 966,664 + +0.7 966,665 - +1.4 966,851 + +0.4 966,852 - +0.1 966,852 - -0.1 966,852 - -1.1 966,852 - +0.7 966,909 - +0.1 966,980 + -0.0 966,981 - +0.5 967,101 + PP_0829 0.22 -0.1 967,101 + PP_0829 0.22 -0.3 967,102 - PP_0829 0.23 +1.6 967,102 - PP_0829 0.23 +0.0 967,106 + PP_0829 0.24 +0.1 967,118 - PP_0829 0.28 -0.3 967,118 - PP_0829 0.28 +0.8 967,187 + PP_0829 0.51 -1.9 967,187 + PP_0829 0.51 -0.8 967,187 + PP_0829 0.51 +0.2 967,187 + PP_0829 0.51 -0.9 967,187 + PP_0829 0.51 +0.6 967,187 + PP_0829 0.51 -0.5 967,187 + PP_0829 0.51 -0.4 967,187 + PP_0829 0.51 +0.4 967,188 - PP_0829 0.52 +0.6 967,188 - PP_0829 0.52 +0.7 967,188 - PP_0829 0.52 -1.9 967,188 - PP_0829 0.52 +1.0 967,188 - PP_0829 0.52 -2.1 967,188 - PP_0829 0.52 -0.7 967,188 - PP_0829 0.52 -2.1 967,188 - PP_0829 0.52 -0.8 967,188 - PP_0829 0.52 -0.9 967,188 - PP_0829 0.52 +1.0 967,224 + PP_0829 0.64 +0.2 967,225 - PP_0829 0.64 -0.3 967,225 - PP_0829 0.64 +1.4 967,225 - PP_0829 0.64 +0.2 967,267 + PP_0829 0.78 -0.6 967,267 + PP_0829 0.78 +0.2 967,267 + PP_0829 0.78 +0.6 967,267 + PP_0829 0.78 +1.4 967,267 + PP_0829 0.78 +0.7 967,267 + PP_0829 0.78 -0.2 967,267 + PP_0829 0.78 -0.2 967,267 + PP_0829 0.78 -1.6 967,268 - PP_0829 0.78 +0.5 967,268 - PP_0829 0.78 -0.5 967,268 - PP_0829 0.78 +0.6 967,268 - PP_0829 0.78 -1.4 967,268 - PP_0829 0.78 -0.1 967,343 + -0.3 967,351 + -2.0 967,351 + +0.9 967,352 - +0.6 967,352 - -0.4 967,499 + PP_0830 0.33 -0.1 967,499 + PP_0830 0.33 -0.4 967,500 - PP_0830 0.33 -1.8 967,500 - PP_0830 0.33 -0.5 967,500 - PP_0830 0.33 -0.4 967,547 - PP_0830 0.43 -1.0 967,547 - PP_0830 0.43 +0.4 967,579 + PP_0830 0.49 +1.5 967,597 + PP_0830 0.53 -1.3 967,597 + PP_0830 0.53 +0.5 967,598 - PP_0830 0.53 -0.4 967,598 - PP_0830 0.53 -0.7 967,598 - PP_0830 0.53 -1.9
Or see this region's nucleotide sequence