Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0795 and PP_0796 are separated by 147 nucleotides PP_0796 and PP_0797 are separated by 72 nucleotides PP_0797 and PP_0798 overlap by 4 nucleotides
PP_0795: PP_0795 - fructose PTS permease - IIBC component, at 913,290 to 915,032
_0795
PP_0796: PP_0796 - conserved protein of unknown function, at 915,180 to 915,467
_0796
PP_0797: PP_0797 - conserved protein of unknown function, at 915,540 to 916,079
_0797
PP_0798: PP_0798 - GGDEF domain protein, at 916,076 to 917,566
_0798
Position (kb) 915
916
917 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 914.608 kb on - strand, within PP_0795 at 914.808 kb on - strand, within PP_0795 at 914.970 kb on + strand at 914.970 kb on + strand at 914.971 kb on - strand at 914.971 kb on - strand at 914.971 kb on - strand at 914.971 kb on - strand at 915.028 kb on + strand at 915.028 kb on + strand at 915.028 kb on + strand at 915.097 kb on + strand at 915.098 kb on - strand at 915.213 kb on + strand, within PP_0796 at 915.214 kb on - strand, within PP_0796 at 915.214 kb on - strand, within PP_0796 at 915.214 kb on - strand, within PP_0796 at 915.311 kb on - strand, within PP_0796 at 915.311 kb on - strand, within PP_0796 at 915.347 kb on - strand, within PP_0796 at 915.347 kb on - strand, within PP_0796 at 915.378 kb on + strand, within PP_0796 at 915.379 kb on - strand, within PP_0796 at 915.379 kb on - strand, within PP_0796 at 915.379 kb on - strand, within PP_0796 at 915.436 kb on + strand, within PP_0796 at 915.580 kb on - strand at 915.634 kb on + strand, within PP_0797 at 915.654 kb on + strand, within PP_0797 at 915.655 kb on - strand, within PP_0797 at 915.655 kb on - strand, within PP_0797 at 915.813 kb on - strand, within PP_0797 at 915.933 kb on + strand, within PP_0797 at 915.965 kb on - strand, within PP_0797 at 915.990 kb on + strand, within PP_0797 at 915.990 kb on + strand, within PP_0797 at 915.990 kb on + strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 915.991 kb on - strand, within PP_0797 at 916.077 kb on + strand at 916.307 kb on + strand, within PP_0798 at 916.307 kb on + strand, within PP_0798 at 916.329 kb on + strand, within PP_0798 at 916.478 kb on - strand, within PP_0798 at 916.483 kb on + strand, within PP_0798 at 916.483 kb on + strand, within PP_0798 at 916.484 kb on - strand, within PP_0798 at 916.731 kb on - strand, within PP_0798 at 916.813 kb on + strand, within PP_0798 at 916.813 kb on + strand, within PP_0798 at 916.860 kb on + strand, within PP_0798 at 916.861 kb on - strand, within PP_0798 at 916.861 kb on - strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.863 kb on + strand, within PP_0798 at 916.864 kb on - strand, within PP_0798 at 916.864 kb on - strand, within PP_0798 at 916.864 kb on - strand, within PP_0798 at 916.987 kb on - strand, within PP_0798 at 917.044 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.062 kb on + strand, within PP_0798 at 917.063 kb on - strand, within PP_0798
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 914,608 - PP_0795 0.76 +0.1 914,808 - PP_0795 0.87 -0.3 914,970 + +0.4 914,970 + -0.2 914,971 - +0.1 914,971 - +0.6 914,971 - +0.5 914,971 - -0.2 915,028 + +0.2 915,028 + +1.5 915,028 + +1.0 915,097 + +0.7 915,098 - +2.2 915,213 + PP_0796 0.11 -0.9 915,214 - PP_0796 0.12 -0.4 915,214 - PP_0796 0.12 -0.3 915,214 - PP_0796 0.12 +0.3 915,311 - PP_0796 0.45 -1.1 915,311 - PP_0796 0.45 +0.1 915,347 - PP_0796 0.58 +1.2 915,347 - PP_0796 0.58 +0.1 915,378 + PP_0796 0.69 +0.7 915,379 - PP_0796 0.69 -1.9 915,379 - PP_0796 0.69 -0.4 915,379 - PP_0796 0.69 -1.9 915,436 + PP_0796 0.89 +0.4 915,580 - -0.9 915,634 + PP_0797 0.17 -0.3 915,654 + PP_0797 0.21 +0.4 915,655 - PP_0797 0.21 +0.2 915,655 - PP_0797 0.21 -1.2 915,813 - PP_0797 0.51 -0.5 915,933 + PP_0797 0.73 -1.5 915,965 - PP_0797 0.79 -1.6 915,990 + PP_0797 0.83 +0.1 915,990 + PP_0797 0.83 -1.1 915,990 + PP_0797 0.83 -0.4 915,991 - PP_0797 0.84 +3.0 915,991 - PP_0797 0.84 -1.9 915,991 - PP_0797 0.84 -0.9 915,991 - PP_0797 0.84 +0.5 916,077 + +0.1 916,307 + PP_0798 0.15 +0.2 916,307 + PP_0798 0.15 -0.8 916,329 + PP_0798 0.17 -1.8 916,478 - PP_0798 0.27 -0.8 916,483 + PP_0798 0.27 -0.6 916,483 + PP_0798 0.27 +0.5 916,484 - PP_0798 0.27 -0.2 916,731 - PP_0798 0.44 +0.3 916,813 + PP_0798 0.49 -0.3 916,813 + PP_0798 0.49 +0.2 916,860 + PP_0798 0.53 +0.6 916,861 - PP_0798 0.53 -1.3 916,861 - PP_0798 0.53 -0.3 916,863 + PP_0798 0.53 -1.3 916,863 + PP_0798 0.53 -0.0 916,863 + +2.6 916,863 + PP_0798 0.53 -0.8 916,863 + PP_0798 0.53 -2.3 916,863 + PP_0798 0.53 +1.2 916,864 - PP_0798 0.53 +0.9 916,864 - PP_0798 0.53 -1.5 916,864 - PP_0798 0.53 -0.8 916,987 - PP_0798 0.61 +0.6 917,044 + PP_0798 0.65 -0.6 917,062 + PP_0798 0.66 -0.6 917,062 + PP_0798 0.66 -0.4 917,062 + PP_0798 0.66 -1.0 917,062 + PP_0798 0.66 -0.4 917,063 - PP_0798 0.66 +0.3
Or see this region's nucleotide sequence