Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0736 and PP_0737 are separated by 104 nucleotides PP_0737 and PP_0738 are separated by 115 nucleotides PP_0738 and PP_0739 overlap by 4 nucleotides
PP_0736: PP_0736 - Glutamate racemase, at 855,150 to 855,947
_0736
PP_0737: PP_0737 - lipid A 3-O-deacylase, at 856,052 to 856,570
_0737
PP_0738: PP_0738 - conserved protein of unknown function, at 856,686 to 857,384
_0738
PP_0739: PP_0739 - deoxyribodipyrimidine photolyase, at 857,381 to 858,823
_0739
Position (kb) 856
857
858 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.915 kb on - strand at 855.972 kb on + strand at 855.973 kb on - strand at 855.994 kb on - strand at 855.994 kb on - strand at 856.023 kb on + strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.024 kb on - strand at 856.052 kb on - strand at 856.253 kb on + strand, within PP_0737 at 856.253 kb on + strand, within PP_0737 at 856.254 kb on - strand, within PP_0737 at 856.259 kb on + strand, within PP_0737 at 856.259 kb on + strand, within PP_0737 at 856.259 kb on + strand, within PP_0737 at 856.341 kb on + strand, within PP_0737 at 856.350 kb on - strand, within PP_0737 at 856.350 kb on - strand, within PP_0737 at 856.533 kb on + strand at 856.533 kb on + strand at 856.534 kb on - strand at 856.534 kb on - strand at 856.553 kb on + strand at 856.554 kb on - strand at 856.611 kb on + strand at 856.612 kb on - strand at 856.622 kb on + strand at 856.623 kb on - strand at 856.623 kb on - strand at 856.634 kb on + strand at 856.635 kb on - strand at 856.668 kb on + strand at 856.668 kb on + strand at 856.668 kb on + strand at 856.668 kb on + strand at 856.669 kb on - strand at 856.669 kb on - strand at 856.709 kb on + strand at 856.709 kb on + strand at 856.709 kb on + strand at 856.710 kb on - strand at 856.710 kb on - strand at 856.710 kb on - strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.712 kb on + strand at 856.713 kb on - strand at 856.852 kb on - strand, within PP_0738 at 856.889 kb on + strand, within PP_0738 at 857.113 kb on + strand, within PP_0738 at 857.113 kb on + strand, within PP_0738 at 857.114 kb on - strand, within PP_0738 at 857.358 kb on - strand at 857.369 kb on + strand at 857.378 kb on + strand at 857.390 kb on + strand at 857.462 kb on + strand at 857.462 kb on + strand at 857.462 kb on + strand at 857.462 kb on + strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.463 kb on - strand at 857.474 kb on + strand at 857.474 kb on + strand at 857.474 kb on + strand at 857.475 kb on - strand at 857.478 kb on + strand at 857.479 kb on - strand at 857.485 kb on + strand at 857.486 kb on - strand at 857.489 kb on - strand at 857.509 kb on + strand at 857.509 kb on + strand at 857.546 kb on + strand, within PP_0739 at 857.628 kb on + strand, within PP_0739 at 857.628 kb on + strand, within PP_0739 at 857.628 kb on + strand, within PP_0739 at 857.971 kb on - strand, within PP_0739 at 857.988 kb on - strand, within PP_0739 at 858.118 kb on + strand, within PP_0739 at 858.119 kb on - strand, within PP_0739 at 858.260 kb on + strand, within PP_0739
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 855,915 - -1.1 855,915 - +1.3 855,915 - -0.8 855,915 - -0.2 855,972 + +0.3 855,973 - -2.1 855,994 - -1.2 855,994 - +0.7 856,023 + -1.3 856,024 - -2.4 856,024 - +0.3 856,024 - -0.9 856,052 - +0.8 856,253 + PP_0737 0.39 +0.3 856,253 + PP_0737 0.39 -0.0 856,254 - PP_0737 0.39 +0.3 856,259 + PP_0737 0.40 -1.2 856,259 + PP_0737 0.40 -1.1 856,259 + PP_0737 0.40 -1.5 856,341 + PP_0737 0.56 -0.9 856,350 - PP_0737 0.57 -0.2 856,350 - PP_0737 0.57 -0.6 856,533 + -0.8 856,533 + +0.4 856,534 - +1.3 856,534 - +0.7 856,553 + +0.1 856,554 - +1.8 856,611 + -0.7 856,612 - -0.4 856,622 + +1.4 856,623 - -1.7 856,623 - +0.6 856,634 + +0.3 856,635 - -1.5 856,668 + -0.1 856,668 + -0.9 856,668 + -1.6 856,668 + +0.1 856,669 - -1.7 856,669 - +0.8 856,709 + -0.2 856,709 + +1.0 856,709 + -1.0 856,710 - -0.1 856,710 - +0.6 856,710 - -0.0 856,712 + -0.7 856,712 + +0.5 856,712 + -1.3 856,712 + -3.0 856,712 + -2.1 856,712 + -1.0 856,713 - -0.1 856,852 - PP_0738 0.24 +0.5 856,889 + PP_0738 0.29 -1.4 857,113 + PP_0738 0.61 -1.2 857,113 + PP_0738 0.61 -1.5 857,114 - PP_0738 0.61 -1.5 857,358 - -1.4 857,369 + +0.7 857,378 + +1.0 857,390 + -0.5 857,462 + +0.2 857,462 + +2.3 857,462 + -2.4 857,462 + -2.1 857,463 - -1.2 857,463 - -2.7 857,463 - +1.3 857,463 - -1.3 857,463 - -0.5 857,474 + -0.3 857,474 + +0.1 857,474 + -0.2 857,475 - -0.2 857,478 + +0.1 857,479 - -0.3 857,485 + -0.5 857,486 - +0.5 857,489 - -0.9 857,509 + -2.0 857,509 + +0.3 857,546 + PP_0739 0.11 -2.0 857,628 + PP_0739 0.17 -0.4 857,628 + PP_0739 0.17 +1.4 857,628 + PP_0739 0.17 -2.4 857,971 - PP_0739 0.41 -2.0 857,988 - PP_0739 0.42 -2.0 858,118 + PP_0739 0.51 +0.9 858,119 - PP_0739 0.51 +0.9 858,260 + PP_0739 0.61 -0.5
Or see this region's nucleotide sequence