Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0699 and PP_t70 are separated by 130 nucleotides PP_t70 and PP_0700 are separated by 95 nucleotides PP_0700 and PP_0701 are separated by 130 nucleotides
PP_0699: PP_0699 - Transporter, LysE family, at 812,479 to 813,093
_0699
PP_t70: PP_t70 - tRNA-Met, at 813,224 to 813,300
_t70
PP_0700: PP_0700 - putative Transmembrane sensor, at 813,396 to 814,382
_0700
PP_0701: PP_0701 - fosmidomycin efflux system, at 814,513 to 815,727
_0701
Position (kb) 813
814
815 Strain fitness (log2 ratio) -2
-1
0
1 at 812.401 kb on - strand at 812.422 kb on + strand at 812.638 kb on + strand, within PP_0699 at 812.638 kb on + strand, within PP_0699 at 812.638 kb on + strand, within PP_0699 at 812.638 kb on + strand, within PP_0699 at 812.639 kb on - strand at 812.639 kb on - strand, within PP_0699 at 812.734 kb on + strand, within PP_0699 at 812.735 kb on - strand, within PP_0699 at 812.735 kb on - strand, within PP_0699 at 812.760 kb on + strand, within PP_0699 at 812.760 kb on + strand, within PP_0699 at 812.798 kb on + strand, within PP_0699 at 812.986 kb on - strand, within PP_0699 at 813.086 kb on + strand at 813.086 kb on + strand at 813.086 kb on + strand at 813.086 kb on + strand at 813.087 kb on - strand at 813.103 kb on - strand at 813.504 kb on + strand, within PP_0700 at 813.505 kb on - strand, within PP_0700 at 813.650 kb on + strand, within PP_0700 at 813.651 kb on - strand, within PP_0700 at 813.651 kb on - strand, within PP_0700 at 813.783 kb on + strand, within PP_0700 at 813.784 kb on - strand, within PP_0700 at 813.802 kb on + strand, within PP_0700 at 813.803 kb on - strand, within PP_0700 at 813.803 kb on - strand, within PP_0700 at 813.804 kb on + strand, within PP_0700 at 813.805 kb on - strand, within PP_0700 at 813.805 kb on - strand, within PP_0700 at 813.972 kb on + strand, within PP_0700 at 813.972 kb on + strand, within PP_0700 at 813.972 kb on + strand, within PP_0700 at 813.973 kb on - strand, within PP_0700 at 814.037 kb on - strand, within PP_0700 at 814.037 kb on - strand, within PP_0700 at 814.042 kb on + strand, within PP_0700 at 814.043 kb on - strand, within PP_0700 at 814.043 kb on - strand, within PP_0700 at 814.180 kb on + strand, within PP_0700 at 814.200 kb on + strand, within PP_0700 at 814.200 kb on + strand, within PP_0700 at 814.201 kb on - strand, within PP_0700 at 814.201 kb on - strand, within PP_0700 at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.453 kb on + strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.454 kb on - strand at 814.457 kb on + strand at 814.457 kb on + strand at 814.458 kb on - strand at 814.458 kb on - strand at 814.458 kb on - strand at 814.461 kb on + strand at 814.462 kb on - strand at 814.524 kb on + strand at 814.584 kb on + strand at 814.584 kb on + strand at 814.584 kb on + strand at 814.584 kb on + strand at 814.585 kb on - strand at 814.672 kb on + strand, within PP_0701 at 814.672 kb on + strand, within PP_0701 at 814.673 kb on - strand, within PP_0701 at 814.806 kb on + strand, within PP_0701 at 814.806 kb on + strand, within PP_0701 at 814.807 kb on - strand, within PP_0701 at 814.807 kb on - strand, within PP_0701 at 814.807 kb on - strand, within PP_0701 at 814.807 kb on - strand, within PP_0701 at 814.917 kb on + strand, within PP_0701 at 815.027 kb on + strand, within PP_0701 at 815.111 kb on - strand, within PP_0701 at 815.227 kb on + strand, within PP_0701 at 815.227 kb on + strand at 815.227 kb on + strand, within PP_0701 at 815.227 kb on + strand, within PP_0701 at 815.227 kb on + strand at 815.228 kb on - strand, within PP_0701 at 815.228 kb on - strand, within PP_0701 at 815.228 kb on - strand, within PP_0701 at 815.228 kb on - strand, within PP_0701 at 815.233 kb on + strand, within PP_0701 at 815.233 kb on + strand, within PP_0701 at 815.233 kb on + strand, within PP_0701 at 815.234 kb on - strand, within PP_0701 at 815.234 kb on - strand, within PP_0701 at 815.255 kb on - strand, within PP_0701 at 815.259 kb on + strand, within PP_0701 at 815.259 kb on + strand, within PP_0701 at 815.260 kb on - strand, within PP_0701 at 815.266 kb on + strand, within PP_0701 at 815.267 kb on - strand, within PP_0701
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 812,401 - -1.5 812,422 + +0.9 812,638 + PP_0699 0.26 -0.0 812,638 + PP_0699 0.26 -1.1 812,638 + PP_0699 0.26 -2.3 812,638 + PP_0699 0.26 +0.0 812,639 - +0.9 812,639 - PP_0699 0.26 +0.4 812,734 + PP_0699 0.41 -1.5 812,735 - PP_0699 0.42 +1.2 812,735 - PP_0699 0.42 -1.2 812,760 + PP_0699 0.46 -0.3 812,760 + PP_0699 0.46 -2.7 812,798 + PP_0699 0.52 -0.1 812,986 - PP_0699 0.82 -0.3 813,086 + -0.5 813,086 + -0.8 813,086 + -1.7 813,086 + -0.3 813,087 - -0.3 813,103 - +1.3 813,504 + PP_0700 0.11 -1.5 813,505 - PP_0700 0.11 +1.5 813,650 + PP_0700 0.26 -0.8 813,651 - PP_0700 0.26 -0.1 813,651 - PP_0700 0.26 -0.6 813,783 + PP_0700 0.39 +0.4 813,784 - PP_0700 0.39 -1.4 813,802 + PP_0700 0.41 -2.4 813,803 - PP_0700 0.41 +0.9 813,803 - PP_0700 0.41 -1.1 813,804 + PP_0700 0.41 +0.4 813,805 - PP_0700 0.41 -1.9 813,805 - PP_0700 0.41 -0.4 813,972 + PP_0700 0.58 +0.5 813,972 + PP_0700 0.58 +0.2 813,972 + PP_0700 0.58 -0.1 813,973 - PP_0700 0.58 -0.7 814,037 - PP_0700 0.65 -0.0 814,037 - PP_0700 0.65 +0.1 814,042 + PP_0700 0.65 -0.2 814,043 - PP_0700 0.66 -0.7 814,043 - PP_0700 0.66 -1.7 814,180 + PP_0700 0.79 -0.3 814,200 + PP_0700 0.81 -1.7 814,200 + PP_0700 0.81 +0.4 814,201 - PP_0700 0.82 +1.3 814,201 - PP_0700 0.82 -2.3 814,453 + +0.0 814,453 + -1.3 814,453 + -0.7 814,453 + +1.3 814,453 + -1.0 814,453 + -1.6 814,453 + -0.1 814,453 + -1.5 814,453 + -0.4 814,453 + +0.1 814,453 + -0.8 814,453 + -1.8 814,453 + -1.7 814,454 - -0.6 814,454 - +0.0 814,454 - -1.1 814,454 - +0.9 814,454 - -0.0 814,454 - -1.7 814,454 - -0.7 814,454 - +1.4 814,454 - +0.8 814,457 + -0.4 814,457 + +0.1 814,458 - -0.0 814,458 - -2.0 814,458 - +0.3 814,461 + +1.6 814,462 - -1.3 814,524 + +1.1 814,584 + +1.1 814,584 + +1.0 814,584 + -1.9 814,584 + +0.3 814,585 - -0.9 814,672 + PP_0701 0.13 +0.4 814,672 + PP_0701 0.13 +0.3 814,673 - PP_0701 0.13 -1.8 814,806 + PP_0701 0.24 -0.8 814,806 + PP_0701 0.24 -0.9 814,807 - PP_0701 0.24 -0.3 814,807 - PP_0701 0.24 -0.4 814,807 - PP_0701 0.24 -0.1 814,807 - PP_0701 0.24 -1.1 814,917 + PP_0701 0.33 +0.6 815,027 + PP_0701 0.42 -1.6 815,111 - PP_0701 0.49 +0.2 815,227 + PP_0701 0.59 +1.6 815,227 + +1.4 815,227 + PP_0701 0.59 +0.4 815,227 + PP_0701 0.59 +0.5 815,227 + +1.0 815,228 - PP_0701 0.59 -0.2 815,228 - PP_0701 0.59 -0.7 815,228 - PP_0701 0.59 -0.3 815,228 - PP_0701 0.59 +0.0 815,233 + PP_0701 0.59 +0.4 815,233 + PP_0701 0.59 +1.1 815,233 + PP_0701 0.59 +0.7 815,234 - PP_0701 0.59 -0.5 815,234 - PP_0701 0.59 +0.5 815,255 - PP_0701 0.61 -1.5 815,259 + PP_0701 0.61 -2.0 815,259 + PP_0701 0.61 +0.8 815,260 - PP_0701 0.61 +0.4 815,266 + PP_0701 0.62 +0.4 815,267 - PP_0701 0.62 -0.0
Or see this region's nucleotide sequence