Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5450 and PP_5451 are separated by 374 nucleotides PP_5451 and PP_0641 are separated by 169 nucleotides PP_0641 and PP_5452 are separated by 982 nucleotides
PP_5450: PP_5450 - exported protein of unknown function, at 751,007 to 751,606
_5450
PP_5451: PP_5451 - protein of unknown function, at 751,981 to 752,286
_5451
PP_0641: PP_0641 - conserved protein of unknown function, at 752,456 to 752,761
_0641
PP_5452: PP_5452 - protein of unknown function, at 753,744 to 753,926
_5452
Position (kb)
752
753 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 751.476 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.480 kb on - strand, within PP_5450 at 751.489 kb on - strand, within PP_5450 at 751.513 kb on + strand, within PP_5450 at 751.555 kb on + strand at 751.556 kb on - strand at 751.556 kb on - strand at 751.556 kb on - strand at 751.580 kb on + strand at 751.609 kb on - strand at 751.609 kb on - strand at 751.621 kb on + strand at 751.622 kb on - strand at 751.647 kb on + strand at 751.647 kb on + strand at 751.648 kb on - strand at 751.648 kb on - strand at 751.752 kb on - strand at 751.770 kb on + strand at 751.770 kb on + strand at 751.771 kb on - strand at 751.771 kb on - strand at 751.797 kb on - strand at 751.829 kb on + strand at 751.830 kb on - strand at 751.903 kb on - strand at 751.903 kb on - strand at 751.911 kb on + strand at 751.911 kb on + strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.912 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.916 kb on - strand at 751.925 kb on - strand at 751.925 kb on - strand at 751.927 kb on + strand at 751.927 kb on + strand at 751.927 kb on + strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.928 kb on - strand at 751.992 kb on - strand at 751.992 kb on - strand at 751.992 kb on - strand at 752.006 kb on + strand at 752.089 kb on + strand, within PP_5451 at 752.090 kb on - strand, within PP_5451 at 752.090 kb on - strand, within PP_5451 at 752.090 kb on - strand, within PP_5451 at 752.127 kb on - strand, within PP_5451 at 752.173 kb on + strand, within PP_5451 at 752.174 kb on - strand, within PP_5451 at 752.264 kb on + strand at 752.284 kb on + strand at 752.352 kb on - strand at 752.427 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.484 kb on + strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.485 kb on - strand at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.487 kb on + strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.488 kb on - strand, within PP_0641 at 752.550 kb on + strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.551 kb on - strand, within PP_0641 at 752.628 kb on - strand, within PP_0641 at 752.700 kb on + strand, within PP_0641 at 752.700 kb on + strand, within PP_0641 at 752.701 kb on - strand, within PP_0641 at 752.701 kb on - strand, within PP_0641 at 752.701 kb on - strand at 752.701 kb on - strand, within PP_0641 at 752.701 kb on - strand, within PP_0641 at 752.745 kb on - strand at 752.849 kb on + strand at 752.850 kb on - strand at 752.850 kb on - strand at 752.942 kb on + strand at 752.976 kb on + strand at 752.976 kb on + strand at 752.977 kb on - strand at 752.977 kb on - strand at 753.005 kb on + strand at 753.005 kb on + strand at 753.005 kb on + strand at 753.006 kb on - strand at 753.009 kb on + strand at 753.009 kb on + strand at 753.071 kb on + strand at 753.071 kb on + strand at 753.072 kb on - strand at 753.206 kb on + strand at 753.207 kb on - strand at 753.207 kb on - strand at 753.207 kb on - strand at 753.216 kb on - strand at 753.222 kb on + strand at 753.223 kb on - strand at 753.228 kb on - strand at 753.231 kb on + strand at 753.231 kb on + strand at 753.231 kb on + strand at 753.232 kb on - strand at 753.238 kb on + strand at 753.239 kb on - strand at 753.241 kb on + strand at 753.241 kb on + strand at 753.251 kb on + strand at 753.409 kb on + strand at 753.436 kb on + strand at 753.436 kb on + strand at 753.437 kb on - strand at 753.437 kb on - strand at 753.437 kb on - strand at 753.440 kb on + strand at 753.440 kb on + strand at 753.440 kb on + strand at 753.441 kb on - strand at 753.465 kb on + strand at 753.466 kb on - strand at 753.518 kb on - strand at 753.624 kb on - strand at 753.759 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 751,476 - PP_5450 0.78 -1.0 751,480 - PP_5450 0.79 +0.9 751,480 - PP_5450 0.79 -0.3 751,480 - PP_5450 0.79 -1.0 751,489 - PP_5450 0.80 +0.8 751,513 + PP_5450 0.84 +0.9 751,555 + +0.6 751,556 - -0.6 751,556 - +0.3 751,556 - +1.9 751,580 + -0.6 751,609 - -0.2 751,609 - -0.4 751,621 + -0.2 751,622 - -1.4 751,647 + -0.4 751,647 + +1.4 751,648 - -1.0 751,648 - -1.0 751,752 - -1.4 751,770 + -1.8 751,770 + +0.6 751,771 - -0.8 751,771 - -1.1 751,797 - -1.8 751,829 + -0.6 751,830 - -0.0 751,903 - -0.1 751,903 - +0.5 751,911 + +0.5 751,911 + -0.8 751,912 - -0.5 751,912 - -0.2 751,912 - +0.2 751,912 - +0.1 751,912 - +1.0 751,912 - +0.4 751,916 - +0.9 751,916 - +0.3 751,916 - +1.0 751,916 - -0.3 751,916 - -0.1 751,925 - +0.6 751,925 - +1.8 751,927 + -0.3 751,927 + -0.7 751,927 + +0.1 751,928 - +0.7 751,928 - -1.4 751,928 - +1.7 751,928 - -0.8 751,928 - -0.5 751,992 - +0.1 751,992 - +0.0 751,992 - +0.1 752,006 + -0.5 752,089 + PP_5451 0.35 +1.1 752,090 - PP_5451 0.36 -1.2 752,090 - PP_5451 0.36 +0.0 752,090 - PP_5451 0.36 +0.1 752,127 - PP_5451 0.48 +0.2 752,173 + PP_5451 0.63 +1.3 752,174 - PP_5451 0.63 -0.0 752,264 + -2.0 752,284 + +0.6 752,352 - -1.9 752,427 + -0.6 752,484 + -0.8 752,484 + +0.0 752,484 + +0.5 752,484 + +0.8 752,484 + -0.3 752,485 - -1.7 752,485 - +0.6 752,485 - +0.1 752,485 - -0.3 752,485 - +0.2 752,487 + PP_0641 0.10 +0.5 752,487 + PP_0641 0.10 -0.6 752,487 + PP_0641 0.10 +0.3 752,487 + PP_0641 0.10 -1.4 752,487 + PP_0641 0.10 -0.2 752,488 - PP_0641 0.10 -0.8 752,488 - PP_0641 0.10 -1.6 752,488 - PP_0641 0.10 -2.1 752,488 - PP_0641 0.10 +0.3 752,488 - PP_0641 0.10 -2.0 752,488 - PP_0641 0.10 -0.8 752,550 + PP_0641 0.31 -0.1 752,551 - PP_0641 0.31 +0.4 752,551 - PP_0641 0.31 -0.2 752,551 - PP_0641 0.31 -0.0 752,551 - PP_0641 0.31 +0.6 752,551 - PP_0641 0.31 +0.3 752,628 - PP_0641 0.56 -1.3 752,700 + PP_0641 0.80 +0.1 752,700 + PP_0641 0.80 +0.8 752,701 - PP_0641 0.80 -1.6 752,701 - PP_0641 0.80 -1.3 752,701 - +1.0 752,701 - PP_0641 0.80 -0.3 752,701 - PP_0641 0.80 -0.6 752,745 - +0.5 752,849 + -0.5 752,850 - +0.5 752,850 - +1.6 752,942 + +1.0 752,976 + +0.1 752,976 + -1.1 752,977 - -2.1 752,977 - -0.3 753,005 + +0.4 753,005 + -2.6 753,005 + +1.2 753,006 - +0.6 753,009 + -1.1 753,009 + -0.0 753,071 + -2.2 753,071 + -0.3 753,072 - -0.8 753,206 + -0.6 753,207 - +0.0 753,207 - +1.1 753,207 - -1.3 753,216 - -0.2 753,222 + -1.1 753,223 - -2.6 753,228 - -0.8 753,231 + -0.1 753,231 + +0.0 753,231 + -0.2 753,232 - +0.0 753,238 + -0.6 753,239 - -0.5 753,241 + -0.5 753,241 + -0.8 753,251 + -0.8 753,409 + -0.2 753,436 + -0.5 753,436 + -1.3 753,437 - +1.1 753,437 - +0.2 753,437 - -0.4 753,440 + -0.5 753,440 + +0.2 753,440 + -2.8 753,441 - +1.5 753,465 + +0.5 753,466 - -0.5 753,518 - +0.6 753,624 - -0.9 753,759 + -1.6
Or see this region's nucleotide sequence