Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0626 and PP_0627 are separated by 84 nucleotides PP_0627 and PP_0628 are separated by 132 nucleotides PP_0628 and PP_5447 are separated by 55 nucleotides
PP_0626: PP_0626 - NADH dehydrogenase, at 734,021 to 735,316
_0626
PP_0627: PP_0627 - conserved protein of unknown function, at 735,401 to 735,574
_0627
PP_0628: PP_0628 - conserved protein of unknown function, at 735,707 to 736,336
_0628
PP_5447: PP_5447 - conserved membrane protein of unknown function, at 736,392 to 736,823
_5447
Position (kb) 735
736 Strain fitness (log2 ratio) -2
-1
0
1
2 at 734.731 kb on - strand, within PP_0626 at 734.731 kb on - strand, within PP_0626 at 734.777 kb on + strand, within PP_0626 at 734.778 kb on - strand, within PP_0626 at 734.807 kb on + strand, within PP_0626 at 734.853 kb on + strand, within PP_0626 at 735.123 kb on + strand, within PP_0626 at 735.123 kb on + strand, within PP_0626 at 735.124 kb on - strand, within PP_0626 at 735.124 kb on - strand, within PP_0626 at 735.251 kb on - strand at 735.322 kb on + strand at 735.329 kb on + strand at 735.329 kb on + strand at 735.330 kb on - strand at 735.330 kb on - strand at 735.330 kb on - strand at 735.405 kb on - strand at 735.405 kb on - strand at 735.405 kb on - strand at 735.528 kb on + strand, within PP_0627 at 735.528 kb on + strand, within PP_0627 at 735.529 kb on - strand, within PP_0627 at 735.529 kb on - strand, within PP_0627 at 735.591 kb on + strand at 735.659 kb on + strand at 735.677 kb on + strand at 735.678 kb on - strand at 735.722 kb on + strand at 735.722 kb on + strand at 735.723 kb on - strand at 735.846 kb on + strand, within PP_0628 at 735.847 kb on - strand, within PP_0628 at 736.204 kb on - strand, within PP_0628 at 736.261 kb on + strand, within PP_0628 at 736.262 kb on - strand, within PP_0628 at 736.306 kb on + strand at 736.307 kb on - strand at 736.307 kb on - strand at 736.320 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.368 kb on + strand at 736.369 kb on - strand at 736.475 kb on + strand, within PP_5447 at 736.475 kb on + strand, within PP_5447 at 736.509 kb on + strand, within PP_5447 at 736.537 kb on + strand, within PP_5447 at 736.537 kb on + strand, within PP_5447 at 736.537 kb on + strand, within PP_5447 at 736.538 kb on - strand, within PP_5447 at 736.538 kb on - strand, within PP_5447
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 734,731 - PP_0626 0.55 +1.8 734,731 - PP_0626 0.55 -0.2 734,777 + PP_0626 0.58 -0.7 734,778 - PP_0626 0.58 -0.7 734,807 + PP_0626 0.61 -0.1 734,853 + PP_0626 0.64 -0.2 735,123 + PP_0626 0.85 +0.1 735,123 + PP_0626 0.85 -0.2 735,124 - PP_0626 0.85 +1.5 735,124 - PP_0626 0.85 -0.1 735,251 - -0.8 735,322 + -1.4 735,329 + +0.8 735,329 + +0.0 735,330 - +1.3 735,330 - -0.2 735,330 - +1.0 735,405 - -0.1 735,405 - -0.1 735,405 - -0.7 735,528 + PP_0627 0.73 -1.5 735,528 + PP_0627 0.73 -0.1 735,529 - PP_0627 0.74 +1.3 735,529 - PP_0627 0.74 +1.4 735,591 + +0.3 735,659 + -0.7 735,677 + +0.7 735,678 - -0.8 735,722 + +1.5 735,722 + -0.5 735,723 - -1.3 735,846 + PP_0628 0.22 -2.3 735,847 - PP_0628 0.22 +1.4 736,204 - PP_0628 0.79 +0.9 736,261 + PP_0628 0.88 +0.4 736,262 - PP_0628 0.88 +0.0 736,306 + +1.2 736,307 - +1.0 736,307 - +0.2 736,320 + -1.5 736,368 + -2.1 736,368 + +1.2 736,368 + -0.6 736,368 + +0.2 736,368 + +0.3 736,368 + -0.4 736,368 + +1.6 736,368 + +0.8 736,369 - +1.8 736,475 + PP_5447 0.19 +0.1 736,475 + PP_5447 0.19 +0.6 736,509 + PP_5447 0.27 -0.5 736,537 + PP_5447 0.34 -1.7 736,537 + PP_5447 0.34 -0.6 736,537 + PP_5447 0.34 +0.1 736,538 - PP_5447 0.34 -0.2 736,538 - PP_5447 0.34 -1.4
Or see this region's nucleotide sequence