Strain Fitness in Pseudomonas putida KT2440 around PP_0493

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0492 and PP_0493 are separated by 144 nucleotidesPP_0493 and PP_0494 overlap by 4 nucleotides PP_0492: PP_0492 - formate dehydrogenase formation protein, at 579,610 to 580,566 _0492 PP_0493: PP_0493 - selenocysteine synthase, at 580,711 to 582,138 _0493 PP_0494: PP_0494 - selenocysteyl-tRNA-specific translation elongation factor, at 582,135 to 584,057 _0494 Position (kb) 580 581 582 583Strain fitness (log2 ratio) -2 -1 0 1 2 3at 579.779 kb on - strand, within PP_0492at 579.779 kb on - strand, within PP_0492at 579.801 kb on - strand, within PP_0492at 579.827 kb on + strand, within PP_0492at 579.956 kb on + strand, within PP_0492at 579.957 kb on - strand, within PP_0492at 580.010 kb on + strand, within PP_0492at 580.010 kb on + strand, within PP_0492at 580.011 kb on - strand, within PP_0492at 580.011 kb on - strand, within PP_0492at 580.069 kb on + strand, within PP_0492at 580.069 kb on + strand, within PP_0492at 580.258 kb on + strand, within PP_0492at 580.315 kb on + strand, within PP_0492at 580.315 kb on + strand, within PP_0492at 580.316 kb on - strand, within PP_0492at 580.435 kb on + strand, within PP_0492at 580.435 kb on + strand, within PP_0492at 580.436 kb on - strand, within PP_0492at 580.436 kb on - strand, within PP_0492at 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.499 kb on + strandat 580.500 kb on - strandat 580.500 kb on - strandat 580.500 kb on - strandat 580.500 kb on - strandat 580.500 kb on - strandat 580.572 kb on + strandat 580.617 kb on + strandat 580.617 kb on + strandat 580.618 kb on - strandat 580.623 kb on + strandat 580.624 kb on - strandat 580.624 kb on - strandat 580.624 kb on - strandat 580.624 kb on - strandat 580.642 kb on + strandat 580.642 kb on + strandat 580.643 kb on - strandat 580.648 kb on + strandat 580.648 kb on + strandat 580.648 kb on + strandat 580.702 kb on + strandat 580.702 kb on + strandat 580.702 kb on + strandat 580.702 kb on + strandat 580.702 kb on + strandat 580.702 kb on + strandat 580.703 kb on - strandat 580.703 kb on - strandat 580.703 kb on - strandat 580.703 kb on - strandat 580.703 kb on - strandat 580.706 kb on - strandat 580.867 kb on - strand, within PP_0493at 581.193 kb on - strand, within PP_0493at 581.193 kb on - strand, within PP_0493at 581.331 kb on + strand, within PP_0493at 581.332 kb on - strand, within PP_0493at 581.332 kb on - strand, within PP_0493at 581.347 kb on + strand, within PP_0493at 581.404 kb on + strand, within PP_0493at 581.404 kb on + strand, within PP_0493at 581.405 kb on - strand, within PP_0493at 581.405 kb on - strand, within PP_0493at 581.436 kb on + strand, within PP_0493at 581.437 kb on - strand, within PP_0493at 581.454 kb on + strand, within PP_0493at 581.454 kb on + strand, within PP_0493at 581.455 kb on - strand, within PP_0493at 581.474 kb on + strand, within PP_0493at 581.475 kb on - strand, within PP_0493at 581.517 kb on + strand, within PP_0493at 581.549 kb on + strand, within PP_0493at 582.329 kb on + strand, within PP_0494at 582.380 kb on - strand, within PP_0494at 582.650 kb on + strand, within PP_0494at 582.650 kb on + strand, within PP_0494at 582.651 kb on - strand, within PP_0494at 582.651 kb on - strand, within PP_0494at 582.651 kb on - strand, within PP_0494at 582.758 kb on + strand, within PP_0494at 582.758 kb on + strand, within PP_0494at 582.758 kb on + strand, within PP_0494at 582.758 kb on + strand, within PP_0494at 582.758 kb on + strand, within PP_0494at 582.758 kb on + strand, within PP_0494at 582.759 kb on - strand, within PP_0494at 582.759 kb on - strand, within PP_0494at 582.759 kb on - strand, within PP_0494at 582.963 kb on + strand, within PP_0494at 582.964 kb on - strand, within PP_0494at 582.964 kb on - strand, within PP_0494at 582.971 kb on - strand, within PP_0494at 583.037 kb on - strand, within PP_0494

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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579,779 - PP_0492 0.18 -1.6
579,779 - PP_0492 0.18 +0.5
579,801 - PP_0492 0.20 -1.3
579,827 + PP_0492 0.23 -0.4
579,956 + PP_0492 0.36 -0.4
579,957 - PP_0492 0.36 -1.6
580,010 + PP_0492 0.42 -1.1
580,010 + PP_0492 0.42 +1.7
580,011 - PP_0492 0.42 +0.7
580,011 - PP_0492 0.42 -1.5
580,069 + PP_0492 0.48 -1.4
580,069 + PP_0492 0.48 -0.6
580,258 + PP_0492 0.68 -1.1
580,315 + PP_0492 0.74 -0.8
580,315 + PP_0492 0.74 -0.2
580,316 - PP_0492 0.74 +0.7
580,435 + PP_0492 0.86 -2.2
580,435 + PP_0492 0.86 -1.0
580,436 - PP_0492 0.86 +0.0
580,436 - PP_0492 0.86 +0.3
580,499 + -0.6
580,499 + -0.9
580,499 + +0.5
580,499 + -0.3
580,499 + -1.8
580,499 + +0.6
580,499 + +0.5
580,499 + -0.3
580,499 + +0.0
580,500 - -0.1
580,500 - +0.3
580,500 - -0.4
580,500 - -0.6
580,500 - +1.0
580,572 + +0.5
580,617 + +1.5
580,617 + +0.1
580,618 - +1.9
580,623 + -0.7
580,624 - -0.9
580,624 - -0.4
580,624 - +0.5
580,624 - +0.4
580,642 + -1.0
580,642 + -0.2
580,643 - -0.8
580,648 + -2.0
580,648 + -0.3
580,648 + -2.1
580,702 + -0.1
580,702 + +0.1
580,702 + -0.7
580,702 + +0.5
580,702 + -1.7
580,702 + -1.2
580,703 - +1.2
580,703 - -0.9
580,703 - +2.9
580,703 - -0.9
580,703 - -1.9
580,706 - -0.0
580,867 - PP_0493 0.11 +0.6
581,193 - PP_0493 0.34 +0.2
581,193 - PP_0493 0.34 -0.2
581,331 + PP_0493 0.43 +1.4
581,332 - PP_0493 0.43 +1.0
581,332 - PP_0493 0.43 +1.6
581,347 + PP_0493 0.45 +1.0
581,404 + PP_0493 0.49 +1.6
581,404 + PP_0493 0.49 -0.6
581,405 - PP_0493 0.49 -1.4
581,405 - PP_0493 0.49 +0.4
581,436 + PP_0493 0.51 -0.8
581,437 - PP_0493 0.51 +0.7
581,454 + PP_0493 0.52 -1.2
581,454 + PP_0493 0.52 -0.4
581,455 - PP_0493 0.52 -0.6
581,474 + PP_0493 0.53 -2.1
581,475 - PP_0493 0.54 +0.7
581,517 + PP_0493 0.56 +0.3
581,549 + PP_0493 0.59 +1.0
582,329 + PP_0494 0.10 -0.4
582,380 - PP_0494 0.13 -0.7
582,650 + PP_0494 0.27 +0.5
582,650 + PP_0494 0.27 +1.8
582,651 - PP_0494 0.27 -0.0
582,651 - PP_0494 0.27 +0.6
582,651 - PP_0494 0.27 -1.1
582,758 + PP_0494 0.32 +0.8
582,758 + PP_0494 0.32 -0.1
582,758 + PP_0494 0.32 -0.0
582,758 + PP_0494 0.32 -1.6
582,758 + PP_0494 0.32 +0.6
582,758 + PP_0494 0.32 +0.2
582,759 - PP_0494 0.32 -1.1
582,759 - PP_0494 0.32 +0.2
582,759 - PP_0494 0.32 -1.6
582,963 + PP_0494 0.43 -2.3
582,964 - PP_0494 0.43 +0.6
582,964 - PP_0494 0.43 -1.0
582,971 - PP_0494 0.43 -0.7
583,037 - PP_0494 0.47 +0.0

Or see this region's nucleotide sequence