Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0391 and PP_0392 are separated by 74 nucleotides PP_0392 and PP_0393 overlap by 10 nucleotides PP_0393 and PP_0394 are separated by 109 nucleotides
PP_0391: PP_0391 - Glycerol-3-phosphate acyltransferase, at 476,752 to 477,321
_0391
PP_0392: PP_0392 - dihydroneopterin aldolase, at 477,396 to 477,752
_0392
PP_0393: PP_0393 - putative 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 477,743 to 478,252
_0393
PP_0394: PP_0394 - tRNA nucleotidyltransferase - 2',3'-cyclic phosphodiesterase - 2' nucleotidase and phosphatase, at 478,362 to 479,468
_0394
Position (kb) 477
478
479 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 476.827 kb on + strand, within PP_0391 at 476.927 kb on + strand, within PP_0391 at 476.927 kb on + strand, within PP_0391 at 476.928 kb on - strand, within PP_0391 at 476.975 kb on + strand, within PP_0391 at 476.976 kb on - strand, within PP_0391 at 477.035 kb on + strand, within PP_0391 at 477.067 kb on + strand, within PP_0391 at 477.068 kb on - strand, within PP_0391 at 477.068 kb on - strand, within PP_0391 at 477.121 kb on + strand, within PP_0391 at 477.121 kb on + strand, within PP_0391 at 477.122 kb on - strand, within PP_0391 at 477.122 kb on - strand, within PP_0391 at 477.152 kb on - strand, within PP_0391 at 477.175 kb on - strand, within PP_0391 at 477.190 kb on + strand, within PP_0391 at 477.191 kb on - strand, within PP_0391 at 477.191 kb on - strand, within PP_0391 at 477.191 kb on - strand, within PP_0391 at 477.761 kb on + strand at 477.762 kb on - strand at 477.987 kb on - strand, within PP_0393 at 478.034 kb on + strand, within PP_0393 at 478.034 kb on + strand, within PP_0393 at 478.034 kb on + strand, within PP_0393 at 478.035 kb on - strand, within PP_0393 at 478.035 kb on - strand, within PP_0393 at 478.035 kb on - strand, within PP_0393 at 478.052 kb on - strand, within PP_0393 at 478.069 kb on - strand, within PP_0393 at 478.069 kb on - strand, within PP_0393 at 478.201 kb on - strand, within PP_0393 at 478.327 kb on + strand at 478.327 kb on + strand at 478.328 kb on - strand at 478.328 kb on - strand at 478.357 kb on + strand at 478.363 kb on + strand at 478.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 476,827 + PP_0391 0.13 -0.1 476,927 + PP_0391 0.31 +1.3 476,927 + PP_0391 0.31 -0.3 476,928 - PP_0391 0.31 -2.5 476,975 + PP_0391 0.39 +0.0 476,976 - PP_0391 0.39 +0.8 477,035 + PP_0391 0.50 -0.7 477,067 + PP_0391 0.55 -2.4 477,068 - PP_0391 0.55 +1.3 477,068 - PP_0391 0.55 -0.0 477,121 + PP_0391 0.65 -0.7 477,121 + PP_0391 0.65 -2.8 477,122 - PP_0391 0.65 -1.0 477,122 - PP_0391 0.65 +0.8 477,152 - PP_0391 0.70 -0.4 477,175 - PP_0391 0.74 +0.2 477,190 + PP_0391 0.77 +0.2 477,191 - PP_0391 0.77 -1.8 477,191 - PP_0391 0.77 -0.1 477,191 - PP_0391 0.77 +0.1 477,761 + -0.2 477,762 - +2.5 477,987 - PP_0393 0.48 +0.7 478,034 + PP_0393 0.57 +2.1 478,034 + PP_0393 0.57 +0.3 478,034 + PP_0393 0.57 -1.2 478,035 - PP_0393 0.57 +0.5 478,035 - PP_0393 0.57 +0.1 478,035 - PP_0393 0.57 +0.9 478,052 - PP_0393 0.61 -0.5 478,069 - PP_0393 0.64 +0.1 478,069 - PP_0393 0.64 +1.0 478,201 - PP_0393 0.90 -0.7 478,327 + -1.1 478,327 + -0.6 478,328 - -0.0 478,328 - -0.5 478,357 + -0.4 478,363 + +1.0 478,376 - +0.4
Or see this region's nucleotide sequence