Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0338 and PP_0339 are separated by 13 nucleotides PP_0339 and PP_0340 are separated by 383 nucleotides
PP_0338: PP_0338 - AceF-S-acetyldihydrolipoate, at 406,364 to 408,004
_0338
PP_0339: PP_0339 - Pyruvate dehydrogenase E1 component, at 408,018 to 410,663
_0339
PP_0340: PP_0340 - Glutamate-ammonia-ligase adenylyltransferase, at 411,047 to 413,980
_0340
Position (kb) 408
409
410
411 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 407.163 kb on + strand, within PP_0338 at 408.006 kb on + strand at 408.069 kb on - strand at 408.271 kb on + strand at 408.507 kb on + strand, within PP_0339 at 408.547 kb on + strand, within PP_0339 at 408.626 kb on - strand, within PP_0339 at 408.626 kb on - strand, within PP_0339 at 408.628 kb on + strand at 408.701 kb on - strand, within PP_0339 at 408.712 kb on + strand, within PP_0339 at 408.713 kb on - strand, within PP_0339 at 408.713 kb on - strand at 408.867 kb on - strand, within PP_0339 at 408.931 kb on + strand, within PP_0339 at 408.932 kb on - strand, within PP_0339 at 408.932 kb on - strand, within PP_0339 at 409.072 kb on + strand, within PP_0339 at 409.073 kb on - strand, within PP_0339 at 409.073 kb on - strand, within PP_0339 at 409.112 kb on + strand, within PP_0339 at 409.299 kb on + strand, within PP_0339 at 409.300 kb on - strand, within PP_0339 at 409.337 kb on - strand, within PP_0339 at 409.564 kb on + strand, within PP_0339 at 409.565 kb on - strand, within PP_0339 at 409.684 kb on + strand, within PP_0339 at 409.705 kb on + strand, within PP_0339 at 409.706 kb on - strand, within PP_0339 at 409.714 kb on + strand at 410.051 kb on - strand, within PP_0339 at 410.329 kb on + strand, within PP_0339 at 410.329 kb on + strand, within PP_0339 at 410.330 kb on - strand, within PP_0339 at 410.354 kb on + strand, within PP_0339 at 410.811 kb on + strand at 411.041 kb on + strand at 411.054 kb on + strand at 411.400 kb on + strand, within PP_0340 at 411.401 kb on - strand, within PP_0340 at 411.416 kb on - strand, within PP_0340 at 411.604 kb on + strand, within PP_0340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 407,163 + PP_0338 0.49 +0.5 408,006 + +1.3 408,069 - +0.7 408,271 + +4.5 408,507 + PP_0339 0.18 +2.5 408,547 + PP_0339 0.20 +2.5 408,626 - PP_0339 0.23 +1.9 408,626 - PP_0339 0.23 +3.5 408,628 + +2.9 408,701 - PP_0339 0.26 +1.9 408,712 + PP_0339 0.26 +0.5 408,713 - PP_0339 0.26 +0.6 408,713 - +1.5 408,867 - PP_0339 0.32 +2.5 408,931 + PP_0339 0.35 +4.5 408,932 - PP_0339 0.35 +1.7 408,932 - PP_0339 0.35 +0.9 409,072 + PP_0339 0.40 +3.5 409,073 - PP_0339 0.40 +0.9 409,073 - PP_0339 0.40 +4.5 409,112 + PP_0339 0.41 +0.6 409,299 + PP_0339 0.48 +1.0 409,300 - PP_0339 0.48 +2.9 409,337 - PP_0339 0.50 +2.5 409,564 + PP_0339 0.58 +1.5 409,565 - PP_0339 0.58 +1.9 409,684 + PP_0339 0.63 +0.9 409,705 + PP_0339 0.64 +2.5 409,706 - PP_0339 0.64 +3.5 409,714 + +1.7 410,051 - PP_0339 0.77 +2.5 410,329 + PP_0339 0.87 +1.3 410,329 + PP_0339 0.87 +2.9 410,330 - PP_0339 0.87 +3.5 410,354 + PP_0339 0.88 +1.5 410,811 + +1.7 411,041 + +1.0 411,054 + +0.2 411,400 + PP_0340 0.12 -0.4 411,401 - PP_0340 0.12 -1.2 411,416 - PP_0340 0.13 -0.1 411,604 + PP_0340 0.19 -1.7
Or see this region's nucleotide sequence