Strain Fitness in Pseudomonas putida KT2440 around PP_0302

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPP_0300 and PP_0301 are separated by 99 nucleotidesPP_0301 and PP_0302 are separated by 10 nucleotidesPP_0302 and PP_0303 are separated by 166 nucleotides PP_0300: PP_0300 - putative metallopeptidase, at 360,350 to 361,936 _0300 PP_0301: PP_0301 - betainyl-CoA thiolase, at 362,036 to 362,506 _0301 PP_0302: PP_0302 - L-carnitine 3-dehydrogenase (EC 1.1.1.108) (from data), at 362,517 to 363,482 _0302 PP_0303: PP_0303 - dehydrocarnitine cleavage enzyme, at 363,649 to 364,533 _0303 Position (kb) 362 363 364Strain fitness (log2 ratio) -2 -1 0 1 2at 361.538 kb on - strand, within PP_0300at 361.628 kb on + strand, within PP_0300at 361.738 kb on - strand, within PP_0300at 361.805 kb on - strandat 361.854 kb on + strandat 361.855 kb on - strandat 361.917 kb on - strandat 361.928 kb on + strandat 361.957 kb on + strandat 361.958 kb on - strandat 362.000 kb on + strandat 362.005 kb on - strandat 362.005 kb on - strandat 362.005 kb on - strandat 362.005 kb on - strandat 362.056 kb on + strandat 362.066 kb on - strandat 362.067 kb on + strandat 362.068 kb on - strandat 362.068 kb on - strandat 362.100 kb on - strand, within PP_0301at 362.174 kb on + strand, within PP_0301at 362.175 kb on - strand, within PP_0301at 362.232 kb on + strand, within PP_0301at 362.233 kb on - strand, within PP_0301at 362.233 kb on - strand, within PP_0301at 362.313 kb on + strand, within PP_0301at 362.314 kb on - strand, within PP_0301at 362.321 kb on - strand, within PP_0301at 362.409 kb on + strand, within PP_0301at 362.409 kb on + strand, within PP_0301at 362.427 kb on + strand, within PP_0301at 362.454 kb on + strand, within PP_0301at 362.519 kb on - strandat 362.519 kb on - strandat 362.695 kb on + strand, within PP_0302at 362.695 kb on + strand, within PP_0302at 362.696 kb on - strand, within PP_0302at 362.696 kb on - strand, within PP_0302at 362.771 kb on + strand, within PP_0302at 362.771 kb on + strand, within PP_0302at 362.773 kb on + strand, within PP_0302at 362.773 kb on + strand, within PP_0302at 362.773 kb on + strand, within PP_0302at 362.774 kb on - strand, within PP_0302at 362.774 kb on - strand, within PP_0302at 362.774 kb on - strand, within PP_0302at 362.774 kb on - strand, within PP_0302at 362.774 kb on - strand, within PP_0302at 362.774 kb on - strand, within PP_0302at 362.777 kb on + strand, within PP_0302at 362.777 kb on + strand, within PP_0302at 362.777 kb on + strand, within PP_0302at 362.778 kb on - strand, within PP_0302at 362.778 kb on - strand, within PP_0302at 362.778 kb on - strand, within PP_0302at 362.778 kb on - strand, within PP_0302at 362.778 kb on - strand, within PP_0302at 362.969 kb on - strand, within PP_0302at 362.969 kb on - strand, within PP_0302at 362.991 kb on - strand, within PP_0302at 362.991 kb on - strand, within PP_0302at 363.044 kb on - strand, within PP_0302at 363.044 kb on - strand, within PP_0302at 363.100 kb on + strand, within PP_0302at 363.100 kb on + strand, within PP_0302at 363.101 kb on - strand, within PP_0302at 363.101 kb on - strand, within PP_0302at 363.482 kb on + strandat 363.482 kb on + strandat 363.482 kb on + strandat 363.483 kb on - strandat 363.483 kb on - strandat 363.483 kb on - strandat 363.499 kb on - strandat 363.508 kb on + strandat 363.508 kb on + strandat 363.508 kb on + strandat 363.508 kb on + strandat 363.508 kb on + strandat 363.509 kb on - strandat 363.509 kb on - strandat 363.509 kb on - strandat 363.780 kb on - strand, within PP_0303at 363.780 kb on - strand, within PP_0303at 363.780 kb on - strand, within PP_0303at 364.109 kb on + strand, within PP_0303at 364.109 kb on + strand, within PP_0303at 364.110 kb on - strand, within PP_0303at 364.110 kb on - strand, within PP_0303at 364.426 kb on + strand, within PP_0303

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
remove
361,538 - PP_0300 0.75 +1.4
361,628 + PP_0300 0.81 -0.3
361,738 - PP_0300 0.87 +0.5
361,805 - +0.5
361,854 + -1.3
361,855 - -1.3
361,917 - +0.1
361,928 + +1.2
361,957 + +1.3
361,958 - -1.0
362,000 + -0.8
362,005 - +1.8
362,005 - +0.3
362,005 - -1.1
362,005 - +0.9
362,056 + +0.2
362,066 - -0.4
362,067 + +0.7
362,068 - +1.2
362,068 - +0.1
362,100 - PP_0301 0.14 -0.7
362,174 + PP_0301 0.29 -0.3
362,175 - PP_0301 0.30 -0.7
362,232 + PP_0301 0.42 +0.1
362,233 - PP_0301 0.42 -0.8
362,233 - PP_0301 0.42 -0.2
362,313 + PP_0301 0.59 +0.1
362,314 - PP_0301 0.59 -1.2
362,321 - PP_0301 0.61 +0.3
362,409 + PP_0301 0.79 -0.3
362,409 + PP_0301 0.79 +1.2
362,427 + PP_0301 0.83 -0.4
362,454 + PP_0301 0.89 -1.5
362,519 - +0.7
362,519 - -0.9
362,695 + PP_0302 0.18 +1.1
362,695 + PP_0302 0.18 +1.7
362,696 - PP_0302 0.19 -0.5
362,696 - PP_0302 0.19 -0.7
362,771 + PP_0302 0.26 -0.8
362,771 + PP_0302 0.26 -0.5
362,773 + PP_0302 0.27 +1.7
362,773 + PP_0302 0.27 -1.9
362,773 + PP_0302 0.27 +0.8
362,774 - PP_0302 0.27 -1.7
362,774 - PP_0302 0.27 +0.2
362,774 - PP_0302 0.27 -0.2
362,774 - PP_0302 0.27 +0.1
362,774 - PP_0302 0.27 -0.6
362,774 - PP_0302 0.27 -1.3
362,777 + PP_0302 0.27 +0.1
362,777 + PP_0302 0.27 +2.5
362,777 + PP_0302 0.27 +0.9
362,778 - PP_0302 0.27 -1.5
362,778 - PP_0302 0.27 +1.2
362,778 - PP_0302 0.27 -0.7
362,778 - PP_0302 0.27 -0.5
362,778 - PP_0302 0.27 +0.1
362,969 - PP_0302 0.47 -2.0
362,969 - PP_0302 0.47 -1.3
362,991 - PP_0302 0.49 +1.2
362,991 - PP_0302 0.49 +0.6
363,044 - PP_0302 0.55 -0.1
363,044 - PP_0302 0.55 -1.0
363,100 + PP_0302 0.60 +1.1
363,100 + PP_0302 0.60 +0.1
363,101 - PP_0302 0.60 +0.6
363,101 - PP_0302 0.60 -0.7
363,482 + +0.2
363,482 + -0.6
363,482 + +0.5
363,483 - -1.8
363,483 - -1.2
363,483 - -0.5
363,499 - -1.3
363,508 + -0.8
363,508 + +0.7
363,508 + -0.6
363,508 + -1.4
363,508 + +0.1
363,509 - +0.6
363,509 - +0.3
363,509 - +0.2
363,780 - PP_0303 0.15 +0.5
363,780 - PP_0303 0.15 -0.9
363,780 - PP_0303 0.15 -0.7
364,109 + PP_0303 0.52 -0.1
364,109 + PP_0303 0.52 -0.9
364,110 - PP_0303 0.52 -1.6
364,110 - PP_0303 0.52 -0.6
364,426 + PP_0303 0.88 -0.6

Or see this region's nucleotide sequence