Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5440 and PP_t72 are separated by 188 nucleotides PP_t72 and PP_0285 are separated by 90 nucleotides PP_0285 and PP_0286 overlap by 4 nucleotides PP_0286 and PP_0287 overlap by 4 nucleotides
PP_5440: PP_5440 - conserved protein of unknown function, at 343,684 to 343,911
_5440
PP_t72: PP_t72 - tRNA-Phe, at 344,100 to 344,175
_t72
PP_0285: PP_0285 - Fe(2+)-trafficking protein, at 344,266 to 344,538
_0285
PP_0286: PP_0286 - adenine glycosylase, at 344,535 to 345,602
_0286
PP_0287: PP_0287 - conserved exported protein of unknown function, at 345,599 to 347,845
_0287
Position (kb) 344
345
346 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.548 kb on + strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.549 kb on - strand at 343.553 kb on - strand at 343.553 kb on - strand at 343.561 kb on + strand at 343.583 kb on + strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.584 kb on - strand at 343.669 kb on + strand at 343.669 kb on + strand at 343.697 kb on - strand at 343.717 kb on + strand, within PP_5440 at 343.717 kb on + strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.739 kb on - strand, within PP_5440 at 343.741 kb on + strand, within PP_5440 at 343.742 kb on - strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.779 kb on + strand, within PP_5440 at 343.780 kb on - strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.852 kb on + strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.853 kb on - strand, within PP_5440 at 343.909 kb on + strand at 343.909 kb on + strand at 343.910 kb on - strand at 343.961 kb on + strand at 343.962 kb on - strand at 344.286 kb on - strand at 344.511 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.633 kb on - strand at 344.861 kb on - strand, within PP_0286 at 345.026 kb on + strand, within PP_0286 at 345.064 kb on + strand, within PP_0286 at 345.065 kb on - strand, within PP_0286 at 345.089 kb on + strand, within PP_0286 at 345.111 kb on + strand, within PP_0286 at 345.111 kb on + strand, within PP_0286 at 345.112 kb on - strand, within PP_0286 at 345.112 kb on - strand, within PP_0286 at 345.112 kb on - strand at 345.160 kb on + strand, within PP_0286 at 345.160 kb on + strand, within PP_0286 at 345.161 kb on - strand, within PP_0286 at 345.270 kb on - strand, within PP_0286 at 345.355 kb on + strand, within PP_0286 at 345.355 kb on + strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.356 kb on - strand, within PP_0286 at 345.358 kb on + strand, within PP_0286 at 345.451 kb on + strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.452 kb on - strand, within PP_0286 at 345.562 kb on + strand at 345.810 kb on + strand at 345.810 kb on + strand at 345.811 kb on - strand at 345.811 kb on - strand at 345.882 kb on + strand, within PP_0287 at 345.882 kb on + strand, within PP_0287 at 345.882 kb on + strand, within PP_0287 at 345.882 kb on - strand, within PP_0287 at 345.883 kb on - strand, within PP_0287 at 345.883 kb on - strand, within PP_0287 at 345.883 kb on - strand, within PP_0287 at 346.353 kb on + strand, within PP_0287 at 346.353 kb on + strand, within PP_0287 at 346.353 kb on + strand, within PP_0287 at 346.353 kb on + strand at 346.354 kb on - strand, within PP_0287 at 346.354 kb on - strand, within PP_0287 at 346.436 kb on - strand, within PP_0287
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 343,548 + +1.7 343,548 + -0.2 343,548 + +0.7 343,548 + -0.1 343,548 + -0.1 343,548 + -0.2 343,549 - -1.3 343,549 - +1.0 343,549 - -0.7 343,553 - +0.5 343,553 - +0.4 343,561 + +0.3 343,583 + -0.5 343,584 - -0.4 343,584 - -1.6 343,584 - +0.3 343,584 - +1.5 343,669 + -1.6 343,669 + -1.5 343,697 - +1.3 343,717 + PP_5440 0.14 +1.7 343,717 + PP_5440 0.14 +0.7 343,739 - PP_5440 0.24 -0.4 343,739 - PP_5440 0.24 +1.0 343,739 - PP_5440 0.24 +0.3 343,739 - PP_5440 0.24 -0.2 343,741 + PP_5440 0.25 -0.6 343,742 - PP_5440 0.25 -1.1 343,779 + PP_5440 0.42 -1.3 343,779 + PP_5440 0.42 +1.0 343,780 - PP_5440 0.42 -2.9 343,852 + PP_5440 0.74 +0.5 343,852 + PP_5440 0.74 -0.2 343,853 - PP_5440 0.74 -1.1 343,853 - PP_5440 0.74 +1.5 343,853 - PP_5440 0.74 +0.4 343,909 + -0.6 343,909 + -1.1 343,910 - +0.1 343,961 + -0.2 343,962 - -0.7 344,286 - +0.8 344,511 - -0.9 344,633 - +0.3 344,633 - +1.6 344,633 - +1.8 344,633 - -0.5 344,861 - PP_0286 0.31 -0.6 345,026 + PP_0286 0.46 -0.4 345,064 + PP_0286 0.50 -1.1 345,065 - PP_0286 0.50 +0.9 345,089 + PP_0286 0.52 +0.3 345,111 + PP_0286 0.54 +1.0 345,111 + PP_0286 0.54 -0.3 345,112 - PP_0286 0.54 -2.0 345,112 - PP_0286 0.54 -1.2 345,112 - +3.3 345,160 + PP_0286 0.59 -2.0 345,160 + PP_0286 0.59 -2.3 345,161 - PP_0286 0.59 -2.8 345,270 - PP_0286 0.69 +1.3 345,355 + PP_0286 0.77 -0.9 345,355 + PP_0286 0.77 -0.2 345,356 - PP_0286 0.77 +0.2 345,356 - PP_0286 0.77 -0.6 345,356 - PP_0286 0.77 +0.2 345,358 + PP_0286 0.77 -0.4 345,451 + PP_0286 0.86 -1.1 345,452 - PP_0286 0.86 +0.5 345,452 - PP_0286 0.86 -1.5 345,452 - PP_0286 0.86 +0.6 345,562 + +0.4 345,810 + -0.3 345,810 + -1.2 345,811 - +0.6 345,811 - -0.9 345,882 + PP_0287 0.13 +1.9 345,882 + PP_0287 0.13 -0.8 345,882 + PP_0287 0.13 -1.4 345,882 - PP_0287 0.13 +1.1 345,883 - PP_0287 0.13 -1.9 345,883 - PP_0287 0.13 -1.6 345,883 - PP_0287 0.13 -1.0 346,353 + PP_0287 0.34 +0.0 346,353 + PP_0287 0.34 +0.2 346,353 + PP_0287 0.34 +0.8 346,353 + +0.5 346,354 - PP_0287 0.34 -0.7 346,354 - PP_0287 0.34 +0.2 346,436 - PP_0287 0.37 +1.0
Or see this region's nucleotide sequence