Strain Fitness in Pseudomonas putida KT2440 around PP_0183

Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0180 and PP_0181 are separated by 62 nucleotidesPP_0181 and PP_0182 are separated by 69 nucleotidesPP_0182 and PP_0183 are separated by 148 nucleotidesPP_0183 and PP_0184 are separated by 124 nucleotides PP_0180: PP_0180 - putative cytochrome c family protein, at 231,902 to 233,812 _0180 PP_0181: PP_0181 - conserved protein of unknown function, at 233,875 to 234,120 _0181 PP_0182: PP_0182 - conserved protein of unknown function, at 234,190 to 234,492 _0182 PP_0183: PP_0183 - glutathione S-transferase, at 234,641 to 235,303 _0183 PP_0184: PP_0184 - Argininosuccinate lyase, at 235,428 to 236,834 _0184 Position (kb) 234 235 236Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 233.717 kb on + strandat 233.718 kb on - strandat 233.766 kb on - strandat 233.908 kb on + strand, within PP_0181at 233.908 kb on + strand, within PP_0181at 233.908 kb on + strand, within PP_0181at 233.909 kb on - strand, within PP_0181at 233.954 kb on + strand, within PP_0181at 234.037 kb on + strand, within PP_0181at 234.038 kb on - strand, within PP_0181at 234.082 kb on + strand, within PP_0181at 234.254 kb on + strand, within PP_0182at 234.254 kb on + strand, within PP_0182at 234.255 kb on - strand, within PP_0182at 234.283 kb on + strand, within PP_0182at 234.283 kb on + strand, within PP_0182at 234.505 kb on - strandat 234.505 kb on - strandat 234.505 kb on - strandat 234.505 kb on - strandat 234.533 kb on + strandat 234.533 kb on + strandat 234.596 kb on + strandat 234.596 kb on + strandat 234.597 kb on - strandat 234.597 kb on - strandat 234.598 kb on + strandat 234.599 kb on - strandat 234.599 kb on - strandat 234.599 kb on - strandat 234.599 kb on - strandat 234.674 kb on + strandat 234.675 kb on - strandat 234.677 kb on + strandat 234.677 kb on + strandat 234.677 kb on + strandat 234.677 kb on + strandat 234.677 kb on + strandat 234.678 kb on - strandat 234.678 kb on - strandat 234.678 kb on - strandat 234.737 kb on + strand, within PP_0183at 234.738 kb on - strand, within PP_0183at 234.842 kb on + strand, within PP_0183at 234.842 kb on + strand, within PP_0183at 234.843 kb on - strand, within PP_0183at 234.843 kb on - strand, within PP_0183at 234.845 kb on - strand, within PP_0183at 234.935 kb on + strand, within PP_0183at 234.935 kb on + strand, within PP_0183at 234.936 kb on - strand, within PP_0183at 234.936 kb on - strand, within PP_0183at 234.955 kb on + strand, within PP_0183at 234.955 kb on + strand, within PP_0183at 234.962 kb on + strand, within PP_0183at 235.073 kb on + strand, within PP_0183at 235.073 kb on + strand, within PP_0183at 235.073 kb on + strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.074 kb on - strand, within PP_0183at 235.373 kb on + strandat 235.373 kb on + strandat 235.627 kb on + strand, within PP_0184at 235.627 kb on + strand, within PP_0184at 235.628 kb on - strand, within PP_0184at 235.628 kb on - strand, within PP_0184at 235.628 kb on - strand, within PP_0184at 235.690 kb on + strand, within PP_0184at 235.690 kb on + strand, within PP_0184at 235.690 kb on - strand, within PP_0184at 235.691 kb on - strand, within PP_0184at 235.858 kb on + strand, within PP_0184at 235.858 kb on + strand, within PP_0184at 235.932 kb on + strand, within PP_0184at 235.933 kb on - strand, within PP_0184at 236.269 kb on + strand, within PP_0184at 236.269 kb on + strand, within PP_0184at 236.269 kb on + strand, within PP_0184at 236.270 kb on - strand, within PP_0184at 236.270 kb on - strand, within PP_0184at 236.270 kb on - strand, within PP_0184

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml
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233,717 + +0.8
233,718 - -0.5
233,766 - -2.5
233,908 + PP_0181 0.13 +1.1
233,908 + PP_0181 0.13 -0.2
233,908 + PP_0181 0.13 -0.5
233,909 - PP_0181 0.14 -0.3
233,954 + PP_0181 0.32 -0.6
234,037 + PP_0181 0.66 -0.2
234,038 - PP_0181 0.66 -1.3
234,082 + PP_0181 0.84 -1.5
234,254 + PP_0182 0.21 -1.0
234,254 + PP_0182 0.21 -0.1
234,255 - PP_0182 0.21 -0.8
234,283 + PP_0182 0.31 +1.2
234,283 + PP_0182 0.31 -0.1
234,505 - -0.3
234,505 - -0.0
234,505 - -0.2
234,505 - +0.2
234,533 + -0.7
234,533 + +0.1
234,596 + +0.4
234,596 + -0.4
234,597 - +0.5
234,597 - -0.4
234,598 + -0.4
234,599 - -0.9
234,599 - -1.1
234,599 - +1.2
234,599 - +0.9
234,674 + -0.5
234,675 - -0.5
234,677 + -0.6
234,677 + -0.9
234,677 + -1.1
234,677 + +0.0
234,677 + -0.8
234,678 - +0.7
234,678 - +4.5
234,678 - +0.1
234,737 + PP_0183 0.14 -0.5
234,738 - PP_0183 0.15 -0.9
234,842 + PP_0183 0.30 -1.3
234,842 + PP_0183 0.30 +0.1
234,843 - PP_0183 0.30 +0.2
234,843 - PP_0183 0.30 -1.3
234,845 - PP_0183 0.31 -1.1
234,935 + PP_0183 0.44 +0.5
234,935 + PP_0183 0.44 -0.3
234,936 - PP_0183 0.44 -2.0
234,936 - PP_0183 0.44 +1.1
234,955 + PP_0183 0.47 -1.8
234,955 + PP_0183 0.47 -2.5
234,962 + PP_0183 0.48 -2.7
235,073 + PP_0183 0.65 -0.7
235,073 + PP_0183 0.65 +0.5
235,073 + PP_0183 0.65 -0.4
235,074 - PP_0183 0.65 -1.2
235,074 - PP_0183 0.65 +0.0
235,074 - PP_0183 0.65 +0.6
235,074 - PP_0183 0.65 +0.1
235,074 - PP_0183 0.65 +0.2
235,074 - PP_0183 0.65 -1.8
235,074 - PP_0183 0.65 +1.1
235,074 - PP_0183 0.65 -1.6
235,373 + -0.4
235,373 + -2.6
235,627 + PP_0184 0.14 -0.4
235,627 + PP_0184 0.14 +0.6
235,628 - PP_0184 0.14 -1.3
235,628 - PP_0184 0.14 -0.0
235,628 - PP_0184 0.14 -2.1
235,690 + PP_0184 0.19 -0.4
235,690 + PP_0184 0.19 -2.0
235,690 - PP_0184 0.19 +1.1
235,691 - PP_0184 0.19 -0.1
235,858 + PP_0184 0.31 -0.6
235,858 + PP_0184 0.31 -1.3
235,932 + PP_0184 0.36 -1.7
235,933 - PP_0184 0.36 +1.1
236,269 + PP_0184 0.60 -2.1
236,269 + PP_0184 0.60 +0.1
236,269 + PP_0184 0.60 -1.8
236,270 - PP_0184 0.60 -0.3
236,270 - PP_0184 0.60 +0.2
236,270 - PP_0184 0.60 -0.1

Or see this region's nucleotide sequence