Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0149 and PP_0150 overlap by 4 nucleotides PP_0150 and PP_0151 are separated by 36 nucleotides PP_0151 and PP_0152 are separated by 89 nucleotides PP_0152 and PP_0153 are separated by 245 nucleotides PP_0153 and PP_0154 are separated by 373 nucleotides
PP_0149: PP_0149 - conserved exported protein of unknown function, at 158,433 to 160,388
_0149
PP_0150: PP_0150 - conserved exported protein of unknown function, at 160,385 to 160,705
_0150
PP_0151: PP_0151 - conserved exported protein of unknown function, at 160,742 to 161,035
_0151
PP_0152: PP_0152 - conserved exported protein of unknown function, at 161,125 to 161,505
_0152
PP_0153: PP_0153 - hypothetical protein, at 161,751 to 161,966
_0153
PP_0154: PP_0154 - Succinyl-CoA:acetate CoA-transferase (EC 2.8.3.18) (from data) , at 162,340 to 163,833
_0154
Position (kb)
161
162 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 160.140 kb on + strand, within PP_0149 at 160.140 kb on + strand, within PP_0149 at 160.159 kb on + strand, within PP_0149 at 160.274 kb on + strand at 160.275 kb on - strand at 160.397 kb on + strand at 160.413 kb on - strand at 160.459 kb on + strand, within PP_0150 at 160.460 kb on - strand, within PP_0150 at 160.460 kb on - strand, within PP_0150 at 160.481 kb on + strand, within PP_0150 at 160.739 kb on + strand at 160.739 kb on + strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.740 kb on - strand at 160.743 kb on + strand at 160.743 kb on + strand at 160.744 kb on - strand at 160.744 kb on - strand at 160.808 kb on + strand, within PP_0151 at 160.809 kb on - strand, within PP_0151 at 160.894 kb on + strand, within PP_0151 at 160.894 kb on + strand, within PP_0151 at 160.894 kb on + strand, within PP_0151 at 160.941 kb on + strand, within PP_0151 at 160.942 kb on - strand, within PP_0151 at 161.029 kb on - strand at 161.351 kb on - strand, within PP_0152 at 161.433 kb on + strand, within PP_0152 at 161.434 kb on - strand, within PP_0152 at 161.481 kb on - strand at 161.529 kb on + strand at 161.568 kb on + strand at 161.858 kb on + strand, within PP_0153 at 161.858 kb on + strand, within PP_0153 at 161.859 kb on - strand, within PP_0153 at 161.957 kb on + strand at 161.958 kb on - strand at 161.958 kb on - strand at 161.997 kb on + strand at 161.998 kb on - strand at 162.001 kb on + strand at 162.001 kb on + strand at 162.002 kb on - strand at 162.049 kb on - strand at 162.052 kb on + strand at 162.053 kb on - strand at 162.082 kb on + strand at 162.207 kb on - strand at 162.241 kb on - strand at 162.246 kb on + strand at 162.246 kb on + strand at 162.246 kb on + strand at 162.247 kb on - strand at 162.264 kb on - strand at 162.447 kb on - strand at 162.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 160,140 + PP_0149 0.87 -1.8 160,140 + PP_0149 0.87 -0.8 160,159 + PP_0149 0.88 -1.3 160,274 + +1.1 160,275 - -0.6 160,397 + -0.2 160,413 - -0.6 160,459 + PP_0150 0.23 -2.2 160,460 - PP_0150 0.23 +1.0 160,460 - PP_0150 0.23 -0.1 160,481 + PP_0150 0.30 -0.6 160,739 + +0.5 160,739 + +0.8 160,740 - -0.5 160,740 - +2.1 160,740 - -1.0 160,740 - +0.6 160,740 - -0.5 160,743 + +0.4 160,743 + -0.0 160,744 - -0.6 160,744 - +0.4 160,808 + PP_0151 0.22 +1.2 160,809 - PP_0151 0.23 -0.4 160,894 + PP_0151 0.52 -0.7 160,894 + PP_0151 0.52 +0.4 160,894 + PP_0151 0.52 -0.2 160,941 + PP_0151 0.68 +1.7 160,942 - PP_0151 0.68 -0.0 161,029 - +0.1 161,351 - PP_0152 0.59 -1.7 161,433 + PP_0152 0.81 -1.0 161,434 - PP_0152 0.81 -0.6 161,481 - -0.8 161,529 + +0.5 161,568 + -0.5 161,858 + PP_0153 0.50 +0.1 161,858 + PP_0153 0.50 +0.4 161,859 - PP_0153 0.50 -0.7 161,957 + -2.6 161,958 - -0.3 161,958 - -1.5 161,997 + +0.0 161,998 - +0.4 162,001 + -0.4 162,001 + +0.4 162,002 - +0.3 162,049 - -0.3 162,052 + +0.5 162,053 - +0.2 162,082 + +1.1 162,207 - -0.9 162,241 - -1.1 162,246 + +0.6 162,246 + -1.6 162,246 + -1.3 162,247 - -1.2 162,264 - +0.4 162,447 - +1.3 162,468 - -0.3
Or see this region's nucleotide sequence