Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0124 and PP_0125 are separated by 201 nucleotides PP_0125 and PP_0126 are separated by 31 nucleotides PP_0126 and PP_0127 are separated by 151 nucleotides PP_0127 and PP_0128 are separated by 12 nucleotides
PP_0124: PP_0124 - probable GTP-binding protein, at 130,641 to 131,273
_0124
PP_0125: PP_0125 - putative cytochrome c-type protein, at 131,475 to 131,777
_0125
PP_0126: PP_0126 - Cytochrome c4, at 131,809 to 132,426
_0126
PP_0127: PP_0127 - Thiol:disulfide interchange protein, at 132,578 to 133,210
_0127
PP_0128: PP_0128 - conserved protein of unknown function, at 133,223 to 134,077
_0128
Position (kb)
131
132
133 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 131.355 kb on - strand at 131.355 kb on - strand at 131.356 kb on + strand at 131.356 kb on + strand at 131.356 kb on + strand at 131.357 kb on - strand at 131.357 kb on - strand at 131.357 kb on - strand at 131.357 kb on - strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.358 kb on + strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.359 kb on - strand at 131.424 kb on - strand at 131.424 kb on - strand at 131.424 kb on - strand at 131.502 kb on - strand at 131.502 kb on - strand at 131.502 kb on - strand at 131.532 kb on - strand, within PP_0125 at 131.532 kb on - strand, within PP_0125 at 131.553 kb on + strand, within PP_0125 at 131.554 kb on - strand, within PP_0125 at 131.554 kb on - strand, within PP_0125 at 131.656 kb on + strand, within PP_0125 at 131.772 kb on - strand at 131.775 kb on + strand at 131.775 kb on + strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.776 kb on - strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.789 kb on + strand at 131.790 kb on - strand at 131.803 kb on + strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.804 kb on - strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.819 kb on + strand at 131.820 kb on - strand at 131.820 kb on - strand at 131.877 kb on + strand, within PP_0126 at 131.877 kb on + strand, within PP_0126 at 131.995 kb on + strand, within PP_0126 at 132.019 kb on - strand, within PP_0126 at 132.036 kb on + strand, within PP_0126 at 132.037 kb on - strand, within PP_0126 at 132.103 kb on + strand, within PP_0126 at 132.286 kb on + strand, within PP_0126 at 132.406 kb on + strand at 132.406 kb on + strand at 132.407 kb on - strand at 132.424 kb on + strand at 132.443 kb on + strand at 132.443 kb on + strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.444 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.502 kb on - strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.572 kb on + strand at 132.573 kb on - strand at 132.583 kb on + strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.584 kb on - strand at 132.671 kb on + strand, within PP_0127 at 132.671 kb on + strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.672 kb on - strand, within PP_0127 at 132.740 kb on + strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.080 kb on - strand, within PP_0127 at 133.096 kb on - strand, within PP_0127 at 133.122 kb on - strand, within PP_0127 at 133.208 kb on + strand at 133.208 kb on + strand at 133.208 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.240 kb on + strand at 133.241 kb on - strand at 133.387 kb on + strand, within PP_0128 at 133.387 kb on + strand, within PP_0128 at 133.388 kb on - strand, within PP_0128
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 131,355 - -0.2 131,355 - +0.6 131,356 + +0.8 131,356 + +0.4 131,356 + -0.9 131,357 - +0.1 131,357 - +0.6 131,357 - -0.7 131,357 - -1.2 131,358 + +0.1 131,358 + +0.8 131,358 + +0.5 131,358 + -0.7 131,358 + -1.1 131,358 + -0.2 131,358 + +0.1 131,358 + +0.8 131,358 + -2.0 131,359 - +0.7 131,359 - -0.7 131,359 - +0.9 131,359 - +1.2 131,359 - -0.0 131,359 - +0.1 131,359 - +1.1 131,359 - +0.1 131,359 - +0.6 131,424 - -0.9 131,424 - -0.3 131,424 - -0.4 131,502 - +1.0 131,502 - -1.3 131,502 - +1.2 131,532 - PP_0125 0.19 +1.6 131,532 - PP_0125 0.19 -1.7 131,553 + PP_0125 0.26 +0.8 131,554 - PP_0125 0.26 +1.3 131,554 - PP_0125 0.26 +1.2 131,656 + PP_0125 0.60 +0.9 131,772 - -1.0 131,775 + +1.5 131,775 + +0.6 131,776 - -1.2 131,776 - +0.0 131,776 - -1.6 131,776 - +0.3 131,789 + -0.5 131,789 + -1.4 131,789 + -0.7 131,790 - -1.3 131,803 + -1.6 131,804 - -2.3 131,804 - -0.5 131,804 - +0.9 131,804 - -0.4 131,819 + -2.1 131,819 + +1.0 131,819 + +1.3 131,820 - -1.1 131,820 - +1.5 131,877 + PP_0126 0.11 +2.3 131,877 + PP_0126 0.11 -1.6 131,995 + PP_0126 0.30 +0.5 132,019 - PP_0126 0.34 +0.5 132,036 + PP_0126 0.37 +1.4 132,037 - PP_0126 0.37 -1.2 132,103 + PP_0126 0.48 -0.4 132,286 + PP_0126 0.77 +1.6 132,406 + +2.2 132,406 + -1.6 132,407 - -0.1 132,424 + +0.4 132,443 + +0.1 132,443 + -0.1 132,444 - -0.4 132,444 - -0.3 132,444 - +0.6 132,444 - -0.7 132,502 - -1.5 132,502 - -0.6 132,502 - -0.7 132,502 - +0.4 132,572 + -0.2 132,572 + -1.3 132,572 + -1.7 132,573 - -0.3 132,583 + +0.2 132,584 - -0.2 132,584 - -1.1 132,584 - -1.0 132,671 + PP_0127 0.15 -0.5 132,671 + PP_0127 0.15 +0.7 132,672 - PP_0127 0.15 -1.2 132,672 - PP_0127 0.15 +0.5 132,672 - PP_0127 0.15 -1.6 132,740 + PP_0127 0.26 -2.0 133,080 - PP_0127 0.79 -0.8 133,080 - PP_0127 0.79 -0.8 133,096 - PP_0127 0.82 -1.6 133,122 - PP_0127 0.86 -0.9 133,208 + -1.5 133,208 + +0.1 133,208 + +0.2 133,240 + +0.1 133,240 + -0.9 133,240 + -0.6 133,240 + +0.7 133,240 + +2.1 133,241 - -1.6 133,387 + PP_0128 0.19 +0.0 133,387 + PP_0128 0.19 +0.3 133,388 - PP_0128 0.19 -0.6
Or see this region's nucleotide sequence