Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0100 and PP_0101 are separated by 207 nucleotides PP_0101 and PP_5426 are separated by 147 nucleotides
PP_0100: PP_0100 - carbonic anhydrase, at 105,207 to 105,926
_0100
PP_0101: PP_0101 - putative sulfate transporter, at 106,134 to 107,651
_0101
PP_5426: PP_5426 - conserved protein of unknown function, at 107,799 to 108,440
_5426
Position (kb)
106
107
108 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 105.148 kb on + strand at 105.149 kb on - strand at 105.149 kb on - strand at 105.179 kb on + strand at 105.179 kb on + strand at 105.183 kb on + strand at 105.922 kb on - strand at 105.924 kb on + strand at 105.924 kb on + strand at 105.924 kb on + strand at 105.924 kb on + strand at 105.925 kb on - strand at 105.954 kb on + strand at 106.101 kb on - strand at 106.127 kb on + strand at 106.127 kb on + strand at 106.128 kb on - strand at 106.128 kb on - strand at 106.128 kb on - strand at 106.251 kb on - strand at 106.333 kb on + strand, within PP_0101 at 106.334 kb on - strand, within PP_0101 at 106.470 kb on + strand, within PP_0101 at 106.717 kb on + strand, within PP_0101 at 106.718 kb on - strand, within PP_0101 at 106.743 kb on - strand, within PP_0101 at 106.743 kb on - strand, within PP_0101 at 106.850 kb on + strand, within PP_0101 at 106.851 kb on - strand, within PP_0101 at 106.921 kb on + strand, within PP_0101 at 106.921 kb on + strand, within PP_0101 at 107.030 kb on - strand, within PP_0101 at 107.250 kb on - strand, within PP_0101 at 107.254 kb on - strand, within PP_0101 at 107.276 kb on + strand, within PP_0101 at 107.302 kb on + strand, within PP_0101 at 107.302 kb on + strand, within PP_0101 at 107.303 kb on - strand, within PP_0101 at 107.303 kb on - strand, within PP_0101 at 107.460 kb on - strand, within PP_0101 at 107.508 kb on + strand at 107.508 kb on + strand at 107.509 kb on - strand at 107.509 kb on - strand at 107.509 kb on - strand at 107.509 kb on - strand at 107.510 kb on + strand at 107.511 kb on - strand at 107.511 kb on - strand at 107.511 kb on - strand at 107.555 kb on + strand at 107.555 kb on + strand at 107.650 kb on - strand at 107.672 kb on - strand at 107.749 kb on + strand at 107.749 kb on + strand at 107.749 kb on + strand at 107.781 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.795 kb on + strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.796 kb on - strand at 107.797 kb on + strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.798 kb on - strand at 107.896 kb on + strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 107.934 kb on - strand, within PP_5426 at 108.067 kb on + strand, within PP_5426 at 108.068 kb on - strand, within PP_5426 at 108.106 kb on + strand, within PP_5426 at 108.107 kb on - strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.135 kb on + strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.136 kb on - strand, within PP_5426 at 108.172 kb on + strand, within PP_5426 at 108.173 kb on - strand, within PP_5426 at 108.192 kb on + strand, within PP_5426 at 108.274 kb on + strand, within PP_5426 at 108.274 kb on + strand, within PP_5426 at 108.274 kb on + strand, within PP_5426 at 108.275 kb on - strand, within PP_5426 at 108.412 kb on - strand at 108.412 kb on - strand at 108.458 kb on + strand at 108.458 kb on + strand at 108.459 kb on - strand at 108.459 kb on - strand at 108.459 kb on - strand at 108.459 kb on - strand at 108.480 kb on - strand at 108.485 kb on - strand at 108.493 kb on - strand at 108.494 kb on + strand at 108.495 kb on - strand at 108.513 kb on - strand at 108.522 kb on - strand at 108.522 kb on - strand at 108.522 kb on - strand at 108.566 kb on + strand at 108.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 105,148 + -0.2 105,149 - -1.4 105,149 - -0.3 105,179 + +0.4 105,179 + +1.1 105,183 + +0.3 105,922 - -0.4 105,924 + +1.0 105,924 + -0.1 105,924 + -1.4 105,924 + +0.8 105,925 - +0.8 105,954 + +0.7 106,101 - +0.6 106,127 + +0.4 106,127 + -0.7 106,128 - +0.1 106,128 - -2.1 106,128 - +0.5 106,251 - +0.1 106,333 + PP_0101 0.13 +0.0 106,334 - PP_0101 0.13 +0.7 106,470 + PP_0101 0.22 +0.8 106,717 + PP_0101 0.38 -2.0 106,718 - PP_0101 0.38 -0.2 106,743 - PP_0101 0.40 +0.0 106,743 - PP_0101 0.40 +0.7 106,850 + PP_0101 0.47 +0.9 106,851 - PP_0101 0.47 +0.2 106,921 + PP_0101 0.52 -1.0 106,921 + PP_0101 0.52 +0.5 107,030 - PP_0101 0.59 +0.9 107,250 - PP_0101 0.74 -1.0 107,254 - PP_0101 0.74 +2.0 107,276 + PP_0101 0.75 +1.1 107,302 + PP_0101 0.77 -1.0 107,302 + PP_0101 0.77 -0.1 107,303 - PP_0101 0.77 +0.8 107,303 - PP_0101 0.77 +3.1 107,460 - PP_0101 0.87 -0.8 107,508 + -0.2 107,508 + -0.8 107,509 - +0.1 107,509 - +0.4 107,509 - +0.4 107,509 - +1.0 107,510 + -1.2 107,511 - +0.0 107,511 - -2.1 107,511 - +0.2 107,555 + +0.4 107,555 + +1.2 107,650 - -0.2 107,672 - +0.4 107,749 + -1.3 107,749 + -0.4 107,749 + -0.2 107,781 + +0.8 107,795 + -0.6 107,795 + +0.4 107,795 + -1.2 107,795 + +0.0 107,795 + -1.1 107,795 + +0.4 107,795 + +0.6 107,795 + +0.6 107,796 - +2.5 107,796 - -1.4 107,796 - -0.9 107,796 - -0.2 107,796 - +1.2 107,797 + -1.0 107,798 - +1.1 107,798 - -1.2 107,798 - -1.4 107,798 - +0.4 107,798 - -0.7 107,798 - -2.5 107,896 + PP_5426 0.15 -2.5 107,934 - PP_5426 0.21 -0.9 107,934 - PP_5426 0.21 -0.8 107,934 - PP_5426 0.21 -0.5 107,934 - PP_5426 0.21 -0.4 108,067 + PP_5426 0.42 -1.3 108,068 - PP_5426 0.42 -2.8 108,106 + PP_5426 0.48 +1.3 108,107 - PP_5426 0.48 +0.2 108,135 + PP_5426 0.52 -1.0 108,135 + PP_5426 0.52 -0.5 108,135 + PP_5426 0.52 -2.9 108,135 + PP_5426 0.52 -0.4 108,135 + PP_5426 0.52 +0.2 108,136 - PP_5426 0.52 +3.4 108,136 - PP_5426 0.52 -1.7 108,136 - PP_5426 0.52 -0.3 108,136 - PP_5426 0.52 +1.1 108,172 + PP_5426 0.58 -1.8 108,173 - PP_5426 0.58 -0.3 108,192 + PP_5426 0.61 -1.8 108,274 + PP_5426 0.74 -1.9 108,274 + PP_5426 0.74 +0.4 108,274 + PP_5426 0.74 -2.0 108,275 - PP_5426 0.74 +1.2 108,412 - -0.9 108,412 - -2.1 108,458 + +0.4 108,458 + -0.8 108,459 - -0.2 108,459 - +0.7 108,459 - +0.4 108,459 - +0.2 108,480 - -1.6 108,485 - -0.8 108,493 - -2.2 108,494 + -0.2 108,495 - -0.2 108,513 - -0.3 108,522 - +0.9 108,522 - -0.7 108,522 - +1.2 108,566 + +2.9 108,567 - -0.4
Or see this region's nucleotide sequence