Experiment: R2A with Polymyxin B sulfate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0004 and PP_0005 are separated by 630 nucleotides PP_0005 and PP_0006 are separated by 88 nucleotides
PP_0004: PP_0004 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme, at 2,486 to 4,381
_0004
PP_0005: PP_0005 - GTPase, at 5,012 to 6,382
_0005
PP_0006: PP_0006 - probable membrane protein insertase, at 6,471 to 8,153
_0006
Position (kb)
5
6
7 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4.416 kb on - strand at 4.428 kb on - strand at 4.428 kb on - strand at 4.428 kb on - strand at 4.544 kb on + strand at 4.550 kb on + strand at 4.550 kb on + strand at 4.551 kb on - strand at 4.590 kb on + strand at 4.603 kb on + strand at 4.603 kb on + strand at 4.604 kb on - strand at 4.614 kb on + strand at 4.615 kb on - strand at 4.672 kb on + strand at 4.673 kb on - strand at 4.723 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.853 kb on + strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.854 kb on - strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.878 kb on + strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.879 kb on - strand at 4.979 kb on - strand at 5.014 kb on - strand at 6.397 kb on - strand at 6.415 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.462 kb on - strand at 6.465 kb on - strand at 6.492 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.002 mg/ml remove 4,416 - -0.3 4,428 - +1.0 4,428 - -0.1 4,428 - -2.2 4,544 + +2.2 4,550 + +1.2 4,550 + -1.7 4,551 - +1.1 4,590 + -1.9 4,603 + -1.7 4,603 + -2.3 4,604 - -2.0 4,614 + -0.6 4,615 - +2.7 4,672 + +1.9 4,673 - +0.6 4,723 + +0.3 4,853 + -0.7 4,853 + -0.0 4,853 + -1.0 4,853 + -1.1 4,853 + -1.6 4,853 + -1.4 4,853 + -0.8 4,854 - -0.5 4,854 - -0.4 4,854 - -0.2 4,854 - -3.1 4,854 - -3.0 4,854 - -1.5 4,854 - -0.8 4,878 + -1.9 4,878 + -1.3 4,878 + -1.1 4,878 + +0.1 4,878 + +1.2 4,878 + +1.9 4,879 - -0.9 4,879 - +0.5 4,879 - +1.1 4,879 - +1.0 4,879 - -0.7 4,979 - -0.1 5,014 - +1.0 6,397 - +2.1 6,415 - -0.0 6,462 - +0.6 6,462 - -1.4 6,462 - -1.7 6,462 - -1.2 6,462 - +0.4 6,462 - -0.2 6,465 - -2.2 6,492 - +0.5
Or see this region's nucleotide sequence