Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5568 and PP_5569 are separated by 33 nucleotides PP_5569 and PP_5571 are separated by 154 nucleotides PP_5571 and PP_5572 are separated by 8 nucleotides PP_5572 and PP_5573 are separated by 703 nucleotides PP_5573 and PP_3102 are separated by 89 nucleotides
PP_5568: PP_5568 - conserved membrane protein of unknown function, at 3,501,614 to 3,502,312
_5568
PP_5569: PP_5569 - protein of unknown function of phage origin, at 3,502,346 to 3,502,549
_5569
PP_5571: PP_5571 - conserved protein of unknown function, at 3,502,704 to 3,503,015
_5571
PP_5572: PP_5572 - protein of unknown function, at 3,503,024 to 3,503,305
_5572
PP_5573: PP_5573 - protein of unknown function, at 3,504,009 to 3,504,185
_5573
PP_3102: PP_3102 - conserved protein of unknown function, at 3,504,275 to 3,504,571
_3102
Position (kb)
3503
3504 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3502.097 kb on + strand, within PP_5568 at 3502.097 kb on + strand, within PP_5568 at 3502.194 kb on + strand, within PP_5568 at 3502.226 kb on + strand, within PP_5568 at 3502.247 kb on + strand at 3502.260 kb on + strand at 3502.320 kb on + strand at 3502.320 kb on + strand at 3502.392 kb on + strand, within PP_5569 at 3502.402 kb on - strand, within PP_5569 at 3502.414 kb on - strand, within PP_5569 at 3502.478 kb on - strand, within PP_5569 at 3502.478 kb on - strand at 3502.481 kb on + strand, within PP_5569 at 3502.520 kb on - strand, within PP_5569 at 3502.522 kb on - strand, within PP_5569 at 3502.593 kb on + strand at 3502.852 kb on - strand, within PP_5571 at 3503.026 kb on - strand at 3503.069 kb on - strand, within PP_5572 at 3503.154 kb on + strand, within PP_5572 at 3503.169 kb on + strand, within PP_5572 at 3503.169 kb on + strand, within PP_5572 at 3503.195 kb on - strand, within PP_5572 at 3503.427 kb on - strand at 3503.454 kb on - strand at 3503.454 kb on - strand at 3503.586 kb on + strand at 3503.608 kb on - strand at 3503.701 kb on + strand at 3503.762 kb on - strand at 3503.803 kb on - strand at 3503.980 kb on + strand at 3504.010 kb on + strand at 3504.090 kb on + strand, within PP_5573 at 3504.207 kb on + strand at 3504.208 kb on - strand at 3504.208 kb on - strand at 3504.214 kb on + strand at 3504.215 kb on - strand at 3504.216 kb on + strand at 3504.217 kb on - strand at 3504.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,502,097 + PP_5568 0.69 +1.9 3,502,097 + PP_5568 0.69 -0.6 3,502,194 + PP_5568 0.83 +2.8 3,502,226 + PP_5568 0.88 -1.8 3,502,247 + +0.2 3,502,260 + -0.9 3,502,320 + +0.9 3,502,320 + -0.7 3,502,392 + PP_5569 0.23 -3.0 3,502,402 - PP_5569 0.27 -0.4 3,502,414 - PP_5569 0.33 +1.2 3,502,478 - PP_5569 0.65 -0.8 3,502,478 - +0.3 3,502,481 + PP_5569 0.66 -0.1 3,502,520 - PP_5569 0.85 -1.3 3,502,522 - PP_5569 0.86 -1.0 3,502,593 + +2.0 3,502,852 - PP_5571 0.47 +0.3 3,503,026 - -0.9 3,503,069 - PP_5572 0.16 -1.1 3,503,154 + PP_5572 0.46 -0.9 3,503,169 + PP_5572 0.51 -0.7 3,503,169 + PP_5572 0.51 -1.3 3,503,195 - PP_5572 0.61 -0.2 3,503,427 - -0.8 3,503,454 - +0.4 3,503,454 - +0.1 3,503,586 + -0.3 3,503,608 - +0.5 3,503,701 + -0.3 3,503,762 - -0.0 3,503,803 - -0.2 3,503,980 + -0.4 3,504,010 + +0.4 3,504,090 + PP_5573 0.46 -3.1 3,504,207 + +1.1 3,504,208 - -0.2 3,504,208 - -2.0 3,504,214 + -0.3 3,504,215 - -0.3 3,504,216 + +0.1 3,504,217 - -0.0 3,504,217 - +0.4
Or see this region's nucleotide sequence