Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5229 and PP_5230 overlap by 1 nucleotides PP_5230 and PP_5231 overlap by 4 nucleotides PP_5231 and PP_5232 are separated by 99 nucleotides PP_5232 and PP_5233 are separated by 429 nucleotides
PP_5229: PP_5229 - conserved protein of unknown function, at 5,963,201 to 5,963,911
_5229
PP_5230: PP_5230 - Tyrosine recombinase XerC, at 5,963,911 to 5,964,810
_5230
PP_5231: PP_5231 - putative (S)-2-haloacid dehalogenase, at 5,964,807 to 5,965,502
_5231
PP_5232: PP_5232 - conserved protein of unknown function, at 5,965,602 to 5,965,931
_5232
PP_5233: PP_5233 - ammonium transporter AmtB, at 5,966,361 to 5,967,692
_5233
Position (kb)
5964
5965
5966 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5963.813 kb on + strand, within PP_5229 at 5963.813 kb on + strand, within PP_5229 at 5963.813 kb on + strand, within PP_5229 at 5963.813 kb on + strand, within PP_5229 at 5963.814 kb on - strand, within PP_5229 at 5963.846 kb on + strand at 5963.847 kb on - strand at 5963.847 kb on - strand at 5963.847 kb on - strand at 5963.847 kb on - strand at 5963.932 kb on + strand at 5963.932 kb on + strand at 5963.933 kb on - strand at 5963.987 kb on - strand at 5964.151 kb on + strand, within PP_5230 at 5964.158 kb on - strand, within PP_5230 at 5964.239 kb on + strand, within PP_5230 at 5964.331 kb on + strand, within PP_5230 at 5964.344 kb on + strand, within PP_5230 at 5964.344 kb on + strand, within PP_5230 at 5964.410 kb on + strand, within PP_5230 at 5964.410 kb on + strand, within PP_5230 at 5964.411 kb on - strand, within PP_5230 at 5964.411 kb on - strand, within PP_5230 at 5964.481 kb on + strand, within PP_5230 at 5964.724 kb on + strand at 5964.725 kb on - strand at 5964.761 kb on - strand at 5965.050 kb on + strand, within PP_5231 at 5965.170 kb on + strand, within PP_5231 at 5965.170 kb on + strand, within PP_5231 at 5965.170 kb on + strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.171 kb on - strand, within PP_5231 at 5965.231 kb on - strand, within PP_5231 at 5965.558 kb on + strand at 5965.558 kb on + strand at 5965.558 kb on + strand at 5965.622 kb on - strand at 5965.622 kb on - strand at 5965.767 kb on + strand, within PP_5232 at 5965.767 kb on + strand, within PP_5232 at 5965.936 kb on + strand at 5965.936 kb on + strand at 5965.937 kb on - strand at 5965.937 kb on - strand at 5965.937 kb on - strand at 5965.968 kb on + strand at 5965.969 kb on - strand at 5965.969 kb on - strand at 5966.025 kb on + strand at 5966.026 kb on - strand at 5966.109 kb on - strand at 5966.130 kb on - strand at 5966.141 kb on + strand at 5966.142 kb on - strand at 5966.142 kb on - strand at 5966.198 kb on + strand at 5966.199 kb on - strand at 5966.228 kb on + strand at 5966.229 kb on - strand at 5966.229 kb on - strand at 5966.247 kb on - strand at 5966.260 kb on - strand at 5966.260 kb on - strand at 5966.280 kb on - strand at 5966.362 kb on + strand at 5966.362 kb on + strand at 5966.363 kb on - strand at 5966.363 kb on - strand at 5966.363 kb on - strand at 5966.470 kb on + strand at 5966.470 kb on + strand at 5966.470 kb on + strand at 5966.471 kb on - strand at 5966.471 kb on - strand at 5966.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,963,813 + PP_5229 0.86 +1.9 5,963,813 + PP_5229 0.86 -2.6 5,963,813 + PP_5229 0.86 -1.6 5,963,813 + PP_5229 0.86 -0.5 5,963,814 - PP_5229 0.86 -1.9 5,963,846 + +0.6 5,963,847 - -1.1 5,963,847 - -0.4 5,963,847 - -0.6 5,963,847 - -0.7 5,963,932 + -0.6 5,963,932 + +1.2 5,963,933 - -0.4 5,963,987 - -2.6 5,964,151 + PP_5230 0.27 +0.2 5,964,158 - PP_5230 0.27 -0.5 5,964,239 + PP_5230 0.36 -1.3 5,964,331 + PP_5230 0.47 -2.4 5,964,344 + PP_5230 0.48 -0.4 5,964,344 + PP_5230 0.48 -1.0 5,964,410 + PP_5230 0.55 -1.4 5,964,410 + PP_5230 0.55 -1.1 5,964,411 - PP_5230 0.56 -0.9 5,964,411 - PP_5230 0.56 -0.1 5,964,481 + PP_5230 0.63 -2.8 5,964,724 + +0.4 5,964,725 - -0.6 5,964,761 - +0.5 5,965,050 + PP_5231 0.35 +3.2 5,965,170 + PP_5231 0.52 -0.8 5,965,170 + PP_5231 0.52 +1.4 5,965,170 + PP_5231 0.52 -0.9 5,965,171 - PP_5231 0.52 +0.5 5,965,171 - PP_5231 0.52 -1.8 5,965,171 - PP_5231 0.52 -0.4 5,965,171 - PP_5231 0.52 -0.2 5,965,171 - PP_5231 0.52 +2.6 5,965,171 - PP_5231 0.52 -0.0 5,965,171 - PP_5231 0.52 +1.6 5,965,231 - PP_5231 0.61 -0.0 5,965,558 + +0.3 5,965,558 + -1.2 5,965,558 + -0.3 5,965,622 - -0.4 5,965,622 - -0.5 5,965,767 + PP_5232 0.50 +1.9 5,965,767 + PP_5232 0.50 +2.4 5,965,936 + +2.4 5,965,936 + +1.2 5,965,937 - +1.9 5,965,937 - +1.7 5,965,937 - +0.8 5,965,968 + +2.0 5,965,969 - +3.0 5,965,969 - +0.1 5,966,025 + +0.9 5,966,026 - +0.6 5,966,109 - -0.2 5,966,130 - -0.4 5,966,141 + +1.3 5,966,142 - +0.1 5,966,142 - +0.4 5,966,198 + -3.0 5,966,199 - -0.1 5,966,228 + +1.2 5,966,229 - +1.5 5,966,229 - -1.0 5,966,247 - +0.1 5,966,260 - +0.5 5,966,260 - -0.0 5,966,280 - -0.1 5,966,362 + -1.8 5,966,362 + +2.3 5,966,363 - -2.6 5,966,363 - -2.7 5,966,363 - +0.1 5,966,470 + -0.3 5,966,470 + -0.3 5,966,470 + -0.9 5,966,471 - +0.3 5,966,471 - +0.3 5,966,471 - -1.2
Or see this region's nucleotide sequence