Strain Fitness in Pseudomonas putida KT2440 around PP_4651

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
5,274,292	+				+0.1
5,274,292	+				+0.4
5,274,292	+				-0.8
5,274,292	+				+0.7
5,274,293	-				-0.2
5,274,293	-				+0.1
5,274,293	-				-0.4
5,274,293	-				-1.4
5,274,293	-				+0.7
5,274,295	+				-0.0
5,274,295	+				-0.4
5,274,295	+				-1.5
5,274,296	-				-0.6
5,274,309	-				+0.2
5,274,309	-				+0.5
5,274,310	+				-0.9
5,274,310	+				+0.3
5,274,310	+				+0.5
5,274,310	+				-0.0
5,274,310	+				+0.3
5,274,311	-				+0.4
5,274,311	-				-0.8
5,274,311	-				-0.7
5,274,311	-				-0.5
5,274,320	+				-2.2
5,274,342	-				+1.8
5,274,416	+		PP_4650	0.17	+0.3
5,274,417	-		PP_4650	0.17	+0.2
5,274,417	-		PP_4650	0.17	-0.5
5,274,445	+		PP_4650	0.19	-0.1
5,274,446	-		PP_4650	0.20	+0.1
5,274,462	+		PP_4650	0.21	-0.2
5,274,463	-		PP_4650	0.21	+0.5
5,274,487	+		PP_4650	0.24	+0.9
5,274,523	+		PP_4650	0.27	-0.8
5,274,524	-		PP_4650	0.27	+0.5
5,274,746	-		PP_4650	0.49	-1.1
5,274,746	-		PP_4650	0.49	+0.5
5,274,899	+		PP_4650	0.64	-0.4
5,274,900	-		PP_4650	0.65	-0.2
5,275,006	+		PP_4650	0.75	-0.7
5,275,007	-		PP_4650	0.75	-0.8
5,275,030	+		PP_4650	0.77	-1.0
5,275,031	-		PP_4650	0.78	-0.9
5,275,198	+				+0.3
5,275,198	+				+0.2
5,275,198	+				+1.4
5,275,198	+				-0.5
5,275,198	+				-0.8
5,275,199	-				-0.2
5,275,199	-				+0.3
5,275,199	-				-0.2
5,275,199	-				-0.4
5,275,414	+		PP_4651	0.11	-1.0
5,275,414	+		PP_4651	0.11	-0.4
5,275,414	+		PP_4651	0.11	+1.0
5,275,415	-		PP_4651	0.11	-0.3
5,275,445	-		PP_4651	0.13	-0.7
5,275,445	-		PP_4651	0.13	+0.3
5,275,445	-		PP_4651	0.13	+1.0
5,275,486	+		PP_4651	0.16	-0.8
5,275,486	+		PP_4651	0.16	-0.5
5,275,486	+		PP_4651	0.16	-2.4
5,275,486	+		PP_4651	0.16	-1.2
5,275,487	-		PP_4651	0.16	-0.6
5,275,487	-		PP_4651	0.16	+0.3
5,275,487	-		PP_4651	0.16	-1.2
5,275,528	+		PP_4651	0.19	+1.0
5,275,528	+		PP_4651	0.19	-0.3
5,275,529	-		PP_4651	0.19	+0.2
5,275,529	-		PP_4651	0.19	+0.2
5,275,639	+		PP_4651	0.26	-0.4
5,275,640	-		PP_4651	0.26	+0.9
5,275,640	-		PP_4651	0.26	-0.7
5,275,745	+		PP_4651	0.34	+0.3
5,275,850	+		PP_4651	0.41	-2.4
5,275,851	-		PP_4651	0.41	-0.8
5,275,965	-		PP_4651	0.49	-0.8
5,276,270	+				+0.5
5,276,270	+		PP_4651	0.70	-0.6
5,276,271	-		PP_4651	0.70	+0.3
5,276,271	-		PP_4651	0.70	+0.0
5,276,292	+		PP_4651	0.72	+0.4
5,276,391	+		PP_4651	0.79	-1.0
5,276,392	-		PP_4651	0.79	-0.8
5,276,392	-				+1.0
5,276,399	-		PP_4651	0.79	-0.6
5,276,399	-		PP_4651	0.79	-0.8
5,276,399	-		PP_4651	0.79	+0.1
5,276,399	-		PP_4651	0.79	-1.1
5,276,536	+		PP_4651	0.89	+0.3
5,276,536	+		PP_4651	0.89	-0.5
5,276,537	-		PP_4651	0.89	-0.6
5,276,548	+		PP_4651	0.90	-0.1
5,276,549	-		PP_4651	0.90	+0.2
5,276,574	+				+1.4
5,276,676	+				+2.9
5,276,676	+				+0.5
5,276,677	-				+0.7
5,276,677	-				-1.0
5,276,685	+				+0.7
5,276,686	-				-0.2
5,276,715	-				+1.1
5,276,769	+				+0.2
5,276,873	-				-2.0
5,276,873	-				-0.4
5,276,940	+				+0.4
5,276,940	+				-0.5
5,276,941	-				+0.2
5,276,941	-				+0.6
5,276,997	+				-0.5
5,276,997	+				+1.6
5,276,997	+				-0.7
5,276,998	-				-0.3
5,277,014	+				+0.2
5,277,160	+				-1.5
5,277,160	+				+1.4
5,277,161	-				+2.9
5,277,262	+		PP_4652	0.18	+0.0
5,277,263	-		PP_4652	0.18	+0.2
5,277,535	+		PP_4652	0.41	-0.9
5,277,535	+		PP_4652	0.41	+0.6
5,277,536	-		PP_4652	0.41	-0.9
5,277,536	-		PP_4652	0.41	+0.1
5,277,560	-		PP_4652	0.43	+0.1
5,277,580	+				+0.8
5,277,581	-		PP_4652	0.44	-0.6
5,277,581	-		PP_4652	0.44	+0.8
5,277,581	-		PP_4652	0.44	+0.5