Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4348 and PP_4349 are separated by 33 nucleotides PP_4349 and PP_4350 are separated by 10 nucleotides
PP_4348: PP_4348 - putative Cystathionine beta-lyase, at 4,940,549 to 4,941,643
_4348
PP_4349: PP_4349 - conserved protein of unknown function, at 4,941,677 to 4,942,555
_4349
PP_4350: PP_4350 - Aminotransferase, class V, NifS/IscS family, at 4,942,566 to 4,943,729
_4350
Position (kb)
4940
4941
4942 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4939.565 kb on + strand at 4939.598 kb on + strand at 4939.668 kb on + strand at 4939.668 kb on + strand at 4939.696 kb on - strand at 4939.696 kb on - strand at 4939.700 kb on - strand at 4939.754 kb on + strand at 4939.754 kb on + strand at 4940.007 kb on + strand at 4940.007 kb on + strand at 4940.013 kb on + strand at 4940.014 kb on - strand at 4940.014 kb on - strand at 4940.014 kb on - strand at 4940.083 kb on - strand at 4940.185 kb on - strand at 4940.185 kb on - strand at 4940.217 kb on - strand at 4940.224 kb on - strand at 4940.244 kb on + strand at 4940.247 kb on + strand at 4940.394 kb on - strand at 4940.436 kb on - strand at 4940.485 kb on + strand at 4940.485 kb on + strand at 4940.488 kb on + strand at 4940.565 kb on - strand at 4940.565 kb on - strand at 4940.649 kb on - strand at 4940.651 kb on + strand at 4940.810 kb on - strand, within PP_4348 at 4941.051 kb on + strand, within PP_4348 at 4941.051 kb on + strand, within PP_4348 at 4941.167 kb on + strand, within PP_4348 at 4941.298 kb on - strand, within PP_4348 at 4941.301 kb on + strand, within PP_4348 at 4941.301 kb on + strand, within PP_4348 at 4941.518 kb on - strand, within PP_4348 at 4941.523 kb on + strand, within PP_4348 at 4941.712 kb on + strand at 4941.852 kb on + strand, within PP_4349 at 4941.853 kb on - strand, within PP_4349 at 4941.853 kb on - strand, within PP_4349 at 4941.925 kb on + strand, within PP_4349 at 4941.927 kb on + strand, within PP_4349 at 4941.946 kb on + strand, within PP_4349 at 4942.031 kb on + strand, within PP_4349 at 4942.033 kb on + strand, within PP_4349 at 4942.033 kb on + strand, within PP_4349 at 4942.142 kb on - strand, within PP_4349 at 4942.291 kb on - strand, within PP_4349 at 4942.468 kb on - strand at 4942.544 kb on + strand at 4942.587 kb on + strand at 4942.592 kb on - strand at 4942.592 kb on - strand at 4942.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 4,939,565 + +0.3 4,939,598 + +0.5 4,939,668 + +0.3 4,939,668 + +1.3 4,939,696 - +0.3 4,939,696 - -0.5 4,939,700 - +0.2 4,939,754 + +0.2 4,939,754 + -1.5 4,940,007 + +2.3 4,940,007 + -1.3 4,940,013 + -1.3 4,940,014 - -0.4 4,940,014 - +0.4 4,940,014 - -1.3 4,940,083 - -0.4 4,940,185 - +0.6 4,940,185 - -0.7 4,940,217 - +0.3 4,940,224 - -0.9 4,940,244 + +0.3 4,940,247 + +0.6 4,940,394 - +0.9 4,940,436 - +0.5 4,940,485 + +0.7 4,940,485 + -0.6 4,940,488 + +2.9 4,940,565 - +0.8 4,940,565 - -0.0 4,940,649 - -0.0 4,940,651 + -0.8 4,940,810 - PP_4348 0.24 -0.6 4,941,051 + PP_4348 0.46 +2.4 4,941,051 + PP_4348 0.46 +0.5 4,941,167 + PP_4348 0.56 +0.2 4,941,298 - PP_4348 0.68 +4.4 4,941,301 + PP_4348 0.69 +4.2 4,941,301 + PP_4348 0.69 -2.0 4,941,518 - PP_4348 0.88 +1.1 4,941,523 + PP_4348 0.89 +0.1 4,941,712 + +0.2 4,941,852 + PP_4349 0.20 +0.2 4,941,853 - PP_4349 0.20 +0.0 4,941,853 - PP_4349 0.20 -0.6 4,941,925 + PP_4349 0.28 -0.9 4,941,927 + PP_4349 0.28 +0.3 4,941,946 + PP_4349 0.31 +0.7 4,942,031 + PP_4349 0.40 +0.1 4,942,033 + PP_4349 0.41 -0.4 4,942,033 + PP_4349 0.41 -0.8 4,942,142 - PP_4349 0.53 +2.2 4,942,291 - PP_4349 0.70 +0.2 4,942,468 - -0.5 4,942,544 + -0.7 4,942,587 + -0.7 4,942,592 - +0.4 4,942,592 - +0.8 4,942,592 - -1.5
Or see this region's nucleotide sequence