Strain Fitness in Pseudomonas putida KT2440 around PP_4250

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
4,835,113	+				-0.3
4,835,114	-				-0.6
4,835,114	-				+2.5
4,835,114	-				-1.0
4,835,114	-				-0.2
4,835,114	-				+0.1
4,835,114	-				-0.5
4,835,114	-				-0.3
4,835,134	+				-1.2
4,835,135	-				-0.9
4,835,135	-				+0.2
4,835,178	+				-0.6
4,835,179	-				+0.6
4,835,219	+				-0.8
4,835,219	+				-1.1
4,835,219	+				-0.1
4,835,219	+				+1.3
4,835,219	+				-1.5
4,835,219	+				-1.5
4,835,252	+				-0.5
4,835,252	+				-2.3
4,835,253	-				-0.4
4,835,357	-		PP_4249	0.15	+0.5
4,835,477	-		PP_4249	0.33	+1.3
4,835,477	-		PP_4249	0.33	-1.2
4,835,516	+		PP_4249	0.38	-2.5
4,835,717	+		PP_4249	0.67	-2.5
4,835,718	-		PP_4249	0.67	+1.9
4,835,837	+		PP_4249	0.85	-0.4
4,835,837	+		PP_4249	0.85	-0.7
4,835,838	-		PP_4249	0.85	+2.3
4,835,937	+				-0.8
4,835,957	+				-0.8
4,835,958	-				+0.8
4,835,991	+				+3.4
4,835,991	+				-1.7
4,835,991	+				+0.5
4,835,991	+				+0.1
4,835,991	+				+0.9
4,835,992	-				-1.6
4,835,992	-				-2.3
4,835,992	-				-0.1
4,836,019	-				-0.8
4,836,019	-				+2.5
4,836,020	+				-2.0
4,836,020	+				+1.0
4,836,021	-				-0.4
4,836,021	-				-2.8
4,836,021	-				-3.0
4,836,021	-				+0.4
4,836,024	+				-0.6
4,836,024	+				+0.0
4,836,024	+				-0.3
4,836,024	+				+1.1
4,836,025	-				-0.4
4,836,025	-				-0.1
4,836,124	+				+1.4
4,836,173	+		PP_4250	0.13	-0.6
4,836,173	+		PP_4250	0.13	+0.7
4,836,174	-		PP_4250	0.13	-0.6
4,836,225	+		PP_4250	0.16	-0.5
4,836,226	-		PP_4250	0.16	+0.8
4,836,308	+		PP_4250	0.22	+0.6
4,836,414	+		PP_4250	0.29	-1.2
4,836,465	+		PP_4250	0.33	+0.6
4,836,466	-		PP_4250	0.33	-0.5
4,836,466	-		PP_4250	0.33	-1.0
4,836,466	-		PP_4250	0.33	-2.1
4,836,570	+		PP_4250	0.40	+1.2
4,836,607	-		PP_4250	0.43	-1.2
4,836,660	+		PP_4250	0.47	-1.8
4,836,660	+		PP_4250	0.47	+0.9
4,836,660	+		PP_4250	0.47	-0.1
4,836,660	+		PP_4250	0.47	-0.2
4,836,661	-		PP_4250	0.47	-1.4
4,836,661	-		PP_4250	0.47	+0.7
4,836,661	-		PP_4250	0.47	-0.6
4,836,661	-		PP_4250	0.47	-0.2
4,836,663	+		PP_4250	0.47	-2.7
4,836,663	+		PP_4250	0.47	-1.2
4,836,664	-		PP_4250	0.47	-1.5
4,836,666	+		PP_4250	0.47	-0.4
4,836,666	+		PP_4250	0.47	-0.1
4,836,666	+		PP_4250	0.47	-2.0
4,836,666	+		PP_4250	0.47	-0.9
4,836,667	-		PP_4250	0.47	-2.5
4,836,667	-		PP_4250	0.47	-2.9
4,836,667	-		PP_4250	0.47	-0.6
4,836,697	-		PP_4250	0.49	+0.3
4,836,697	-		PP_4250	0.49	-0.6
4,836,697	-		PP_4250	0.49	-1.0
4,836,697	-		PP_4250	0.49	-1.1
4,836,774	+		PP_4250	0.55	-2.6
4,836,774	+		PP_4250	0.55	-0.7
4,836,942	+		PP_4250	0.67	-1.0
4,837,081	-		PP_4250	0.76	+0.8
4,837,119	+		PP_4250	0.79	-0.7
4,837,119	+		PP_4250	0.79	-1.7
4,837,119	+		PP_4250	0.79	-0.2
4,837,120	-		PP_4250	0.79	-0.3
4,837,120	-		PP_4250	0.79	-0.9
4,837,167	-		PP_4250	0.82	-1.8
4,837,177	-		PP_4250	0.83	-1.3
4,837,254	+		PP_4250	0.88	-1.6
4,837,255	-		PP_4250	0.88	+1.5
4,837,484	+				-1.5
4,837,485	-				-0.5
4,837,485	-				-1.0
4,837,606	+		PP_4251	0.29	-0.8
4,837,607	-		PP_4251	0.29	+3.1
4,837,804	+		PP_4251	0.61	-2.7
4,837,804	+		PP_4251	0.61	+0.8
4,837,804	+		PP_4251	0.61	+0.6
4,837,805	-		PP_4251	0.61	+2.5
4,837,846	+		PP_4251	0.68	-2.2
4,837,927	+		PP_4251	0.81	-2.8
4,837,999	+				+1.2
4,838,000	-				+1.6
4,838,228	+				-1.9
4,838,254	+				-0.2
4,838,254	+				-2.5
4,838,255	-				+0.7
4,838,258	+				-1.7
4,838,258	+				+0.3
4,838,258	+				+0.5
4,838,258	+				+1.3
4,838,259	-				-0.9
4,838,259	-				-2.0
4,838,268	+				-2.8
4,838,268	+				-0.5
4,838,268	+				+0.7
4,838,376	+		PP_4253	0.15	-1.8
4,838,377	-		PP_4253	0.15	-2.1
4,838,384	+		PP_4253	0.16	-3.2
4,838,384	+		PP_4253	0.16	-0.5