Strain Fitness in Pseudomonas putida KT2440 around PP_4115

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
4,651,245	+		PP_4114	0.24	+0.7
4,651,245	+		PP_4114	0.24	-1.3
4,651,246	-		PP_4114	0.24	-0.3
4,651,246	-		PP_4114	0.24	-1.7
4,651,353	+		PP_4114	0.33	+1.3
4,651,412	+		PP_4114	0.38	+0.1
4,651,412	+		PP_4114	0.38	-0.3
4,651,412	+		PP_4114	0.38	-0.1
4,651,412	+		PP_4114	0.38	-0.2
4,651,412	+		PP_4114	0.38	+0.2
4,651,413	-		PP_4114	0.38	+0.1
4,651,413	-		PP_4114	0.38	-0.2
4,651,487	+		PP_4114	0.44	-1.1
4,651,488	-		PP_4114	0.44	+0.6
4,651,488	-		PP_4114	0.44	-0.4
4,651,488	-		PP_4114	0.44	-0.7
4,651,488	-		PP_4114	0.44	+2.1
4,651,578	+		PP_4114	0.52	-0.1
4,651,579	-		PP_4114	0.52	-0.9
4,651,579	-		PP_4114	0.52	+0.7
4,651,579	-		PP_4114	0.52	+0.6
4,651,665	+		PP_4114	0.59	-0.7
4,651,665	+		PP_4114	0.59	-0.3
4,651,666	-		PP_4114	0.59	+0.6
4,651,714	+		PP_4114	0.64	+0.3
4,651,714	+		PP_4114	0.64	-0.9
4,651,714	+		PP_4114	0.64	+0.2
4,651,715	-		PP_4114	0.64	-2.1
4,651,717	+		PP_4114	0.64	-2.1
4,651,717	+		PP_4114	0.64	+0.2
4,651,717	+		PP_4114	0.64	-0.2
4,651,717	+		PP_4114	0.64	+0.8
4,651,718	-		PP_4114	0.64	+1.7
4,651,718	-		PP_4114	0.64	-0.9
4,651,718	-		PP_4114	0.64	-0.6
4,651,732	+		PP_4114	0.65	-0.0
4,651,733	-		PP_4114	0.65	-0.4
4,651,733	-		PP_4114	0.65	+0.2
4,651,768	-		PP_4114	0.68	-0.9
4,652,058	-				-1.9
4,652,058	-				-1.1
4,652,119	+				+0.3
4,652,148	+				-1.3
4,652,148	+				-0.1
4,652,149	-				-0.5
4,652,185	-				-0.3
4,652,185	-				-0.4
4,652,205	-				-0.3
4,652,610	+				+0.9
4,652,647	+		PP_4115	0.52	-0.1
4,652,648	-		PP_4115	0.52	+3.3
4,652,725	-		PP_4115	0.62	+0.4
4,652,775	+		PP_4115	0.68	+2.9
4,652,775	+		PP_4115	0.68	-1.0
4,652,775	+		PP_4115	0.68	+0.7
4,652,775	+		PP_4115	0.68	-0.1
4,652,775	+				+1.1
4,652,775	+		PP_4115	0.68	-0.0
4,652,819	-		PP_4115	0.73	+0.2
4,652,973	+				-0.6
4,652,973	+				+1.2
4,652,973	+				-0.2
4,652,974	-				+0.6
4,652,974	-				-0.3
4,652,974	-				-0.7
4,652,974	-				+2.6
4,653,089	+				+0.2
4,653,090	-				+0.0
4,653,102	+				-1.1
4,653,103	-				+0.0
4,653,103	-				+0.1
4,653,103	-				-0.5
4,653,103	-				+3.1
4,653,103	-				-0.4
4,653,103	-				-0.2
4,653,105	+				-0.1
4,653,241	+		PP_5659	0.24	+0.3
4,653,242	-		PP_5659	0.25	+4.3
4,653,243	+		PP_5659	0.25	+0.3
4,653,243	+		PP_5659	0.25	-0.4
4,653,244	-		PP_5659	0.25	+0.6
4,653,490	+				+0.5
4,653,603	+				-1.8
4,653,607	+				-0.4
4,653,607	+				-1.1
4,653,608	-				+0.8
4,653,779	+		PP_4116	0.19	-0.0
4,653,779	+		PP_4116	0.19	+0.7
4,653,779	+		PP_4116	0.19	-0.7
4,653,780	-		PP_4116	0.19	-0.0
4,653,811	+		PP_4116	0.22	-2.1
4,653,811	+		PP_4116	0.22	-0.1
4,653,811	+		PP_4116	0.22	+0.6
4,653,811	+		PP_4116	0.22	+0.5
4,653,811	+		PP_4116	0.22	+0.3
4,653,811	+		PP_4116	0.22	-0.4
4,653,812	-		PP_4116	0.22	+0.8
4,653,812	-		PP_4116	0.22	-1.6
4,653,812	-		PP_4116	0.22	+1.1
4,653,812	-				+0.2
4,653,812	-		PP_4116	0.22	-1.1
4,653,812	-		PP_4116	0.22	-1.1
4,653,862	+		PP_4116	0.26	-0.6
4,653,862	+		PP_4116	0.26	+0.2
4,653,880	+		PP_4116	0.27	+0.7
4,653,985	+		PP_4116	0.35	+1.0
4,653,985	+		PP_4116	0.35	-0.2